Lipoxygenase activities during development of root and nodule of soybean

Detalhes bibliográficos
Autor(a) principal: Junghans, Tatiana Góes
Data de Publicação: 2004
Outros Autores: Oliveira, Maria Goreti de Almeida, Moreira, Maurilio Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6818
Resumo: The objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa.
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spelling Lipoxygenase activities during development of root and nodule of soybeanAtividade de lipoxigenases durante o desenvolvimento da raiz e do nódulo de plantas de sojaGlycine max; triple-null lines; nodulationGlycine max; linhagem triplo nula; nodulaçãoThe objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa.O objetivo deste trabalho foi avaliar lipoxigenases de raízes e nódulos de plantas de soja da variedade Doko e da linhagem triplo nula derivada, desprovida das lipoxigenases da semente, com e sem inoculação de Bradyrhizobium elkanii. A atividade de lipoxigenase foi avaliada em raízes coletadas aos 3, 5, 9, 13, 18 e 28 dias após a inoculação e em nódulos coletados aos 13, 18 e 28 dias após a inoculação. Os perfis de pH-atividade de raiz e nódulo sugerem que o “pool” de lipoxigenases expresso nesses órgãos não difere nos dois genótipos. A atividade de lipoxigenases nas raízes de Doko e Doko triplo nula, com e sem inoculação, declinou com o passar do tempo. A maior atividade de lipoxigenases no início de formação da raiz sugere o envolvimento desta enzima no crescimento e desenvolvimento deste órgão. Nos nódulos houve um aumento acentuado na atividade de lipoxigenase aos 28 dias após a inoculação. Dois grupos de mobilidade com aproximadamente 94 e 97 kDa foram encontrados nos “immunoblottings” para lipoxigenases de raiz e de nódulo.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraJunghans, Tatiana GóesOliveira, Maria Goreti de AlmeidaMoreira, Maurilio Alves2004-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6818Pesquisa Agropecuaria Brasileira; v.39, n.7, jul. 2004; 625-630Pesquisa Agropecuária Brasileira; v.39, n.7, jul. 2004; 625-6301678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/6818/3874info:eu-repo/semantics/openAccess2014-07-23T18:25:42Zoai:ojs.seer.sct.embrapa.br:article/6818Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-23T18:25:42Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Lipoxygenase activities during development of root and nodule of soybean
Atividade de lipoxigenases durante o desenvolvimento da raiz e do nódulo de plantas de soja
title Lipoxygenase activities during development of root and nodule of soybean
spellingShingle Lipoxygenase activities during development of root and nodule of soybean
Junghans, Tatiana Góes
Glycine max; triple-null lines; nodulation
Glycine max; linhagem triplo nula; nodulação
title_short Lipoxygenase activities during development of root and nodule of soybean
title_full Lipoxygenase activities during development of root and nodule of soybean
title_fullStr Lipoxygenase activities during development of root and nodule of soybean
title_full_unstemmed Lipoxygenase activities during development of root and nodule of soybean
title_sort Lipoxygenase activities during development of root and nodule of soybean
author Junghans, Tatiana Góes
author_facet Junghans, Tatiana Góes
Oliveira, Maria Goreti de Almeida
Moreira, Maurilio Alves
author_role author
author2 Oliveira, Maria Goreti de Almeida
Moreira, Maurilio Alves
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Junghans, Tatiana Góes
Oliveira, Maria Goreti de Almeida
Moreira, Maurilio Alves
dc.subject.por.fl_str_mv Glycine max; triple-null lines; nodulation
Glycine max; linhagem triplo nula; nodulação
topic Glycine max; triple-null lines; nodulation
Glycine max; linhagem triplo nula; nodulação
description The objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa.
publishDate 2004
dc.date.none.fl_str_mv 2004-07-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6818
url https://seer.sct.embrapa.br/index.php/pab/article/view/6818
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6818/3874
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.39, n.7, jul. 2004; 625-630
Pesquisa Agropecuária Brasileira; v.39, n.7, jul. 2004; 625-630
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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