Identification of zygotic trifoliate orange seedlings with RAPD molecular markers

Detalhes bibliográficos
Autor(a) principal: Schäfer, Gilmar
Data de Publicação: 2004
Outros Autores: Bastianel, Marinês, Dornelles, Ana Lúcia Cunha
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6752
Resumo: This work aimed to determine germination frequency of zygotic embryos of seeds originated from open pollinated trifoliate orange [Poncirus trifoliata (L.) Raf.], using RAPD molecular markers. Leaves and seeds of P. trifoliata were collected from five commercial nurseries and from a citrus germplasm collection. The seeds were placed to germinate in plastic tubes in a greenhouse, reaching a final sample of 62 seedlings. Nine primers were used and the band patterns generated from seedlings leaves were compared to progenitor-plant-leaf hands. It was possible to identify 3.23% of seedlings originated from zygotic embryos which is acceptable in propagation by seeds in this rootstock.
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spelling Identification of zygotic trifoliate orange seedlings with RAPD molecular markersIdentificação de plântulas zigóticas de trifoliata com o uso de marcadores moleculares RAPDPoncirus trifoliata; rootstocks; citrus; embryoPoncirus trifoliata; porta-enxerto; citros; embriãoThis work aimed to determine germination frequency of zygotic embryos of seeds originated from open pollinated trifoliate orange [Poncirus trifoliata (L.) Raf.], using RAPD molecular markers. Leaves and seeds of P. trifoliata were collected from five commercial nurseries and from a citrus germplasm collection. The seeds were placed to germinate in plastic tubes in a greenhouse, reaching a final sample of 62 seedlings. Nine primers were used and the band patterns generated from seedlings leaves were compared to progenitor-plant-leaf hands. It was possible to identify 3.23% of seedlings originated from zygotic embryos which is acceptable in propagation by seeds in this rootstock.O objetivo deste trabalho foi identificar a freqüência de germinação dos embriões zigóticos de sementes de trifoliata [Poncirus trifoliata (L.) Raf.] oriundas de polinização aberta, com o uso de marcador molecular RAPD. Folhas e sementes de trifoliata foram coletadas de cinco viveiros comerciais e de uma coleção experimental de citros. Essas sementes foram colocadas para germinar em tubetes e casa de vegetação, totalizando uma amostra final de 62 plântulas. Foram utilizadas nove seqüências inicializadoras e os resultados foram analisados a partir da comparação dos padrões das bandas geradas pelas plantas germinadas com os da planta matriz. Identificaram-se 3,23% de plântulas oriundas da germinação do embrião zigótico, valor que é aceitável na propagação por sementes deste porta-enxerto cítrico.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraSchäfer, GilmarBastianel, MarinêsDornelles, Ana Lúcia Cunha2004-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6752Pesquisa Agropecuaria Brasileira; v.39, n.2, fev. 2004; 167-172Pesquisa Agropecuária Brasileira; v.39, n.2, fev. 2004; 167-1721678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/6752/3808info:eu-repo/semantics/openAccess2014-07-03T18:37:16Zoai:ojs.seer.sct.embrapa.br:article/6752Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-03T18:37:16Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Identification of zygotic trifoliate orange seedlings with RAPD molecular markers
Identificação de plântulas zigóticas de trifoliata com o uso de marcadores moleculares RAPD
title Identification of zygotic trifoliate orange seedlings with RAPD molecular markers
spellingShingle Identification of zygotic trifoliate orange seedlings with RAPD molecular markers
Schäfer, Gilmar
Poncirus trifoliata; rootstocks; citrus; embryo
Poncirus trifoliata; porta-enxerto; citros; embrião
title_short Identification of zygotic trifoliate orange seedlings with RAPD molecular markers
title_full Identification of zygotic trifoliate orange seedlings with RAPD molecular markers
title_fullStr Identification of zygotic trifoliate orange seedlings with RAPD molecular markers
title_full_unstemmed Identification of zygotic trifoliate orange seedlings with RAPD molecular markers
title_sort Identification of zygotic trifoliate orange seedlings with RAPD molecular markers
author Schäfer, Gilmar
author_facet Schäfer, Gilmar
Bastianel, Marinês
Dornelles, Ana Lúcia Cunha
author_role author
author2 Bastianel, Marinês
Dornelles, Ana Lúcia Cunha
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Schäfer, Gilmar
Bastianel, Marinês
Dornelles, Ana Lúcia Cunha
dc.subject.por.fl_str_mv Poncirus trifoliata; rootstocks; citrus; embryo
Poncirus trifoliata; porta-enxerto; citros; embrião
topic Poncirus trifoliata; rootstocks; citrus; embryo
Poncirus trifoliata; porta-enxerto; citros; embrião
description This work aimed to determine germination frequency of zygotic embryos of seeds originated from open pollinated trifoliate orange [Poncirus trifoliata (L.) Raf.], using RAPD molecular markers. Leaves and seeds of P. trifoliata were collected from five commercial nurseries and from a citrus germplasm collection. The seeds were placed to germinate in plastic tubes in a greenhouse, reaching a final sample of 62 seedlings. Nine primers were used and the band patterns generated from seedlings leaves were compared to progenitor-plant-leaf hands. It was possible to identify 3.23% of seedlings originated from zygotic embryos which is acceptable in propagation by seeds in this rootstock.
publishDate 2004
dc.date.none.fl_str_mv 2004-02-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6752
url https://seer.sct.embrapa.br/index.php/pab/article/view/6752
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6752/3808
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.39, n.2, fev. 2004; 167-172
Pesquisa Agropecuária Brasileira; v.39, n.2, fev. 2004; 167-172
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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