Genetic diversity among hybrids of 'Cravo' mandarin and 'Pêra' sweet orange

Detalhes bibliográficos
Autor(a) principal: Oliveira, Roberto Pedroso de
Data de Publicação: 2002
Outros Autores: Cristofani, Mariângela, Aguilar-Vildoso, Carlos Ivan, Machado, Marcos Antônio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6364
Resumo: RAPD molecular markers were used to evaluate the genetic diversity in a progeny of 94 hybrids between 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck), obtained by controlled pollination. One hundred two random decamer primers were screened, amplifying 640 fragments, 77.2% monomorphics between the parents. The Jaccard coefficient was used to estimate the genetic similarity among the hybrids, the UPGMA method to establish the phenogram (NTSYS 1.7) and the software BOOD to determine the significance of each cluster. The results indicated that 'Pêra' sweet orange heterozygosity degree is higher than 'Cravo' mandarin, with 180 and 126 loci in heterozygose, respectively. It was observed high genetic similarity between the parents, characterized by the low degree of RAPD polymorphism. Significant statistic groups were not established among the hybrids and the parents, showing that the hybrid genetic constitution was originated by independent RAPD markers segregation. Then, the ligation effect was broken because of the sample size. Hybrids with high genetic similarity degree in relation to 'Cravo' mandarin and 'Pêra' sweet orange could be easily selected using RAPD markers.
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spelling Genetic diversity among hybrids of 'Cravo' mandarin and 'Pêra' sweet orangeDiversidade genética entre híbridos de tangerina 'Cravo' e laranja 'Pêra'citrus fruits; genetic markers; heterozygotes; genetic variationfrutas cítricas; marcadores genéticos; heterozigoto; variação genéticaRAPD molecular markers were used to evaluate the genetic diversity in a progeny of 94 hybrids between 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck), obtained by controlled pollination. One hundred two random decamer primers were screened, amplifying 640 fragments, 77.2% monomorphics between the parents. The Jaccard coefficient was used to estimate the genetic similarity among the hybrids, the UPGMA method to establish the phenogram (NTSYS 1.7) and the software BOOD to determine the significance of each cluster. The results indicated that 'Pêra' sweet orange heterozygosity degree is higher than 'Cravo' mandarin, with 180 and 126 loci in heterozygose, respectively. It was observed high genetic similarity between the parents, characterized by the low degree of RAPD polymorphism. Significant statistic groups were not established among the hybrids and the parents, showing that the hybrid genetic constitution was originated by independent RAPD markers segregation. Then, the ligation effect was broken because of the sample size. Hybrids with high genetic similarity degree in relation to 'Cravo' mandarin and 'Pêra' sweet orange could be easily selected using RAPD markers.Marcadores moleculares RAPD foram utilizados para avaliar a diversidade genética em uma população de 94 híbridos de tangerina 'Cravo' (Citrus reticulata Blanco) com laranja 'Pêra' (C. sinensis (L.) Osbeck), obtidos por polinização controlada. Nas reações de amplificação foram usados 102 "primers" decâmeros de seqüência arbitrária, que amplificaram 640 fragmentos, sendo 77,2% monomórficos entre os parentais. Utilizou-se o coeficiente de Jaccard para estimar a similaridade genética entre os híbridos, o método UPGMA para gerar o fenograma (NTSYS 1,7) e o software BOOD para determinar a consistência de cada agrupamento. Verificou-se que a laranja 'Pêra' apresenta maior heterozigosidade que a tangerina 'Cravo', sendo, respectivamente, 180 e 126 os números observados de loci em heterozigose. Houve alta similaridade genética entre os parentais, caracterizada pelo baixo polimorfismo de marcadores RAPD. Não houve a formação de agrupamentos estatisticamente significativos dos híbridos entre si e com os parentais, demonstrando que a constituição genética dos híbridos foi originada pela segregação independente das marcas RAPD, sendo quebrado o efeito de ligação em função do tamanho amostral em estudo. Híbridos com maior similaridade genética em relação à tangerina 'Cravo' e laranja 'Pêra' podem ser facilmente selecionados a partir de marcadores RAPD.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraOliveira, Roberto Pedroso deCristofani, MariângelaAguilar-Vildoso, Carlos IvanMachado, Marcos Antônio2002-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6364Pesquisa Agropecuaria Brasileira; v.37, n.4, abr. 2002; 479-484Pesquisa Agropecuária Brasileira; v.37, n.4, abr. 2002; 479-4841678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/6364/3421info:eu-repo/semantics/openAccess2010-08-19T17:49:40Zoai:ojs.seer.sct.embrapa.br:article/6364Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2010-08-19T17:49:40Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic diversity among hybrids of 'Cravo' mandarin and 'Pêra' sweet orange
Diversidade genética entre híbridos de tangerina 'Cravo' e laranja 'Pêra'
title Genetic diversity among hybrids of 'Cravo' mandarin and 'Pêra' sweet orange
spellingShingle Genetic diversity among hybrids of 'Cravo' mandarin and 'Pêra' sweet orange
Oliveira, Roberto Pedroso de
citrus fruits; genetic markers; heterozygotes; genetic variation
frutas cítricas; marcadores genéticos; heterozigoto; variação genética
title_short Genetic diversity among hybrids of 'Cravo' mandarin and 'Pêra' sweet orange
title_full Genetic diversity among hybrids of 'Cravo' mandarin and 'Pêra' sweet orange
title_fullStr Genetic diversity among hybrids of 'Cravo' mandarin and 'Pêra' sweet orange
title_full_unstemmed Genetic diversity among hybrids of 'Cravo' mandarin and 'Pêra' sweet orange
title_sort Genetic diversity among hybrids of 'Cravo' mandarin and 'Pêra' sweet orange
author Oliveira, Roberto Pedroso de
author_facet Oliveira, Roberto Pedroso de
Cristofani, Mariângela
Aguilar-Vildoso, Carlos Ivan
Machado, Marcos Antônio
author_role author
author2 Cristofani, Mariângela
Aguilar-Vildoso, Carlos Ivan
Machado, Marcos Antônio
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Oliveira, Roberto Pedroso de
Cristofani, Mariângela
Aguilar-Vildoso, Carlos Ivan
Machado, Marcos Antônio
dc.subject.por.fl_str_mv citrus fruits; genetic markers; heterozygotes; genetic variation
frutas cítricas; marcadores genéticos; heterozigoto; variação genética
topic citrus fruits; genetic markers; heterozygotes; genetic variation
frutas cítricas; marcadores genéticos; heterozigoto; variação genética
description RAPD molecular markers were used to evaluate the genetic diversity in a progeny of 94 hybrids between 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck), obtained by controlled pollination. One hundred two random decamer primers were screened, amplifying 640 fragments, 77.2% monomorphics between the parents. The Jaccard coefficient was used to estimate the genetic similarity among the hybrids, the UPGMA method to establish the phenogram (NTSYS 1.7) and the software BOOD to determine the significance of each cluster. The results indicated that 'Pêra' sweet orange heterozygosity degree is higher than 'Cravo' mandarin, with 180 and 126 loci in heterozygose, respectively. It was observed high genetic similarity between the parents, characterized by the low degree of RAPD polymorphism. Significant statistic groups were not established among the hybrids and the parents, showing that the hybrid genetic constitution was originated by independent RAPD markers segregation. Then, the ligation effect was broken because of the sample size. Hybrids with high genetic similarity degree in relation to 'Cravo' mandarin and 'Pêra' sweet orange could be easily selected using RAPD markers.
publishDate 2002
dc.date.none.fl_str_mv 2002-04-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6364
url https://seer.sct.embrapa.br/index.php/pab/article/view/6364
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6364/3421
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.37, n.4, abr. 2002; 479-484
Pesquisa Agropecuária Brasileira; v.37, n.4, abr. 2002; 479-484
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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