Mixoploidy in napiergrass x pearl millet hybrids treated with antimitotic agents

Detalhes bibliográficos
Autor(a) principal: Abreu, Juscélio Clemente de
Data de Publicação: 2006
Outros Autores: Davide, Lisete Chamma, Pereira, Antônio Vander, Barbosa, Sandro
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7326
Resumo: –The objective of this work was to evaluate methods of chromosome duplication, using antimitotic agents and several botanical materials as explant hybrids between napiergrass (Pennisetum purpureum Shum.) and pearl millet (Pennisetum glaucum (L.) R. Br.). Colchicine (50 mg L-1 and 100 mg L-1) and cycloheximide:8-hydroxyquinoline (1:1) (25 mg L-1:300 mg L-1) solutions have been applied in vivo to shoots and in vitro to seedlings and tillers. The antimitotic effect has been evaluated through survival rate, chromosome number and presence of cell cycle anomalies at the root tips of surviving plants. The best results have been obtained when seedlings have been treated with colchicine and tillers with cycloheximide: 8-hydroxyquinoline. Mixoploidy has been observed in cells having 14 to 42 chromosomes, indicating that duplication, followed by chromosome elimination, has occurred, which has been confirmed by chromosome aberrations. In the average, 86.4% of the analyzed cells have presented a chromosome number different from 21.
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spelling Mixoploidy in napiergrass x pearl millet hybrids treated with antimitotic agentsMixoploidia em híbridos de capim-elefante x milheto tratados com agentes antimitóticosPennisetum purpureum; Pennisetum glaucum; cytogenetic analysis; chromosome elimination; interespecific hybridPennisetum purpureum; Pennisetum glaucum; análise citogenética; eliminação cromossômica; híbrido interespecífico–The objective of this work was to evaluate methods of chromosome duplication, using antimitotic agents and several botanical materials as explant hybrids between napiergrass (Pennisetum purpureum Shum.) and pearl millet (Pennisetum glaucum (L.) R. Br.). Colchicine (50 mg L-1 and 100 mg L-1) and cycloheximide:8-hydroxyquinoline (1:1) (25 mg L-1:300 mg L-1) solutions have been applied in vivo to shoots and in vitro to seedlings and tillers. The antimitotic effect has been evaluated through survival rate, chromosome number and presence of cell cycle anomalies at the root tips of surviving plants. The best results have been obtained when seedlings have been treated with colchicine and tillers with cycloheximide: 8-hydroxyquinoline. Mixoploidy has been observed in cells having 14 to 42 chromosomes, indicating that duplication, followed by chromosome elimination, has occurred, which has been confirmed by chromosome aberrations. In the average, 86.4% of the analyzed cells have presented a chromosome number different from 21.–O objetivo deste trabalho foi avaliar métodos de duplicação cromossômica, com uso de agentes antimitóticos e diversos materiais botânicos como explantes dos híbridos entre capim-elefante (Pennisetum purpureum Schum.) e milheto (Pennisetum glaucum (L.) R. Br.). Utilizaram-se soluções de colchicina a 50 mg L-1 e 100 mg L-1, e de ciclohexamida 25 mg L-1 :8-hidroxiquinoleína 300 mg L-1 (1:1), aplicadas in vitro em segmentos nodais, e in vivo em plântulas e perfilhos, com diferentes períodos de exposição. O efeito dos antimitóticos foi avaliado por meio da taxa de sobrevivência, do número cromossômico e da presença de anomalias no ciclo celular, em meristemas de raízes das plantas sobreviventes. A colchicina apresentou melhor efeito sobre as plântulas, enquanto a ciclohexamida:8-hidroxiquinoleína (1:1) atuou melhor sobre os perfilhos. Observou-se ocorrência de mixoploidia em células que apresentaram de 14 até 42 cromossomos, o que indica que houve duplicação seguida de eliminação cromossômica, confirmada pelas aberrações cromossômicas. Das células analisadas 86,4%, em média, apresentaram número cromossômico diferente de 21.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraAbreu, Juscélio Clemente deDavide, Lisete ChammaPereira, Antônio VanderBarbosa, Sandro2006-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7326Pesquisa Agropecuaria Brasileira; v.41, n.11, nov. 2006; 1629-1635Pesquisa Agropecuária Brasileira; v.41, n.11, nov. 2006; 1629-16351678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7326/4371info:eu-repo/semantics/openAccess2014-10-06T18:05:14Zoai:ojs.seer.sct.embrapa.br:article/7326Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-10-06T18:05:14Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Mixoploidy in napiergrass x pearl millet hybrids treated with antimitotic agents
Mixoploidia em híbridos de capim-elefante x milheto tratados com agentes antimitóticos
title Mixoploidy in napiergrass x pearl millet hybrids treated with antimitotic agents
spellingShingle Mixoploidy in napiergrass x pearl millet hybrids treated with antimitotic agents
Abreu, Juscélio Clemente de
Pennisetum purpureum; Pennisetum glaucum; cytogenetic analysis; chromosome elimination; interespecific hybrid
Pennisetum purpureum; Pennisetum glaucum; análise citogenética; eliminação cromossômica; híbrido interespecífico
title_short Mixoploidy in napiergrass x pearl millet hybrids treated with antimitotic agents
title_full Mixoploidy in napiergrass x pearl millet hybrids treated with antimitotic agents
title_fullStr Mixoploidy in napiergrass x pearl millet hybrids treated with antimitotic agents
title_full_unstemmed Mixoploidy in napiergrass x pearl millet hybrids treated with antimitotic agents
title_sort Mixoploidy in napiergrass x pearl millet hybrids treated with antimitotic agents
author Abreu, Juscélio Clemente de
author_facet Abreu, Juscélio Clemente de
Davide, Lisete Chamma
Pereira, Antônio Vander
Barbosa, Sandro
author_role author
author2 Davide, Lisete Chamma
Pereira, Antônio Vander
Barbosa, Sandro
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Abreu, Juscélio Clemente de
Davide, Lisete Chamma
Pereira, Antônio Vander
Barbosa, Sandro
dc.subject.por.fl_str_mv Pennisetum purpureum; Pennisetum glaucum; cytogenetic analysis; chromosome elimination; interespecific hybrid
Pennisetum purpureum; Pennisetum glaucum; análise citogenética; eliminação cromossômica; híbrido interespecífico
topic Pennisetum purpureum; Pennisetum glaucum; cytogenetic analysis; chromosome elimination; interespecific hybrid
Pennisetum purpureum; Pennisetum glaucum; análise citogenética; eliminação cromossômica; híbrido interespecífico
description –The objective of this work was to evaluate methods of chromosome duplication, using antimitotic agents and several botanical materials as explant hybrids between napiergrass (Pennisetum purpureum Shum.) and pearl millet (Pennisetum glaucum (L.) R. Br.). Colchicine (50 mg L-1 and 100 mg L-1) and cycloheximide:8-hydroxyquinoline (1:1) (25 mg L-1:300 mg L-1) solutions have been applied in vivo to shoots and in vitro to seedlings and tillers. The antimitotic effect has been evaluated through survival rate, chromosome number and presence of cell cycle anomalies at the root tips of surviving plants. The best results have been obtained when seedlings have been treated with colchicine and tillers with cycloheximide: 8-hydroxyquinoline. Mixoploidy has been observed in cells having 14 to 42 chromosomes, indicating that duplication, followed by chromosome elimination, has occurred, which has been confirmed by chromosome aberrations. In the average, 86.4% of the analyzed cells have presented a chromosome number different from 21.
publishDate 2006
dc.date.none.fl_str_mv 2006-11-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7326
url https://seer.sct.embrapa.br/index.php/pab/article/view/7326
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7326/4371
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.41, n.11, nov. 2006; 1629-1635
Pesquisa Agropecuária Brasileira; v.41, n.11, nov. 2006; 1629-1635
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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