Amylase activity in the bullfrog during the post-metamorphic phase

Detalhes bibliográficos
Autor(a) principal: Braga, Luís Gustavo Tavares
Data de Publicação: 2005
Outros Autores: Oliveira, Maria Goreti de Almeida, Lima, William Cardoso, Euclydes, Ricardo Frederico
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7049
Resumo: The objective of this work was to evaluate the amylase activity in the chime of the bullfrog Rana catesbeiana Shaw 1802 (3.6 to 200 g). Eighty-seven animals were distributed in test stalls with controlled temperature and photoperiod. The frogs selected in the post-metamorphic phase received commercial extruded diet ad libitum. During 87 days of the experiment, 29 samples were collected in intervals varying from one to eight days. The intestinal content samples were made by first desensitizing of the frogs in ice and water, and subsequently removing the small intestine. For the tests of amylase activity, commercial enzymatic kits were used. Amylase activity was observed since the third day and it increased until reaching stability, when the animals weighed 4.35 g. Amylase activity (137.44 UI) and specific activity (14,688 UI mg-1) remained stable from the seventh day until the end of the experiment.
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spelling Amylase activity in the bullfrog during the post-metamorphic phaseAtividade da amilase em rã-touro durante a fase pós-metamórficaRana catesbeiana; activity enzymatic; digestive enzymes; frogcultureRana catesbeiana; atividade enzimática; enzimas digestivas; ranicultura The objective of this work was to evaluate the amylase activity in the chime of the bullfrog Rana catesbeiana Shaw 1802 (3.6 to 200 g). Eighty-seven animals were distributed in test stalls with controlled temperature and photoperiod. The frogs selected in the post-metamorphic phase received commercial extruded diet ad libitum. During 87 days of the experiment, 29 samples were collected in intervals varying from one to eight days. The intestinal content samples were made by first desensitizing of the frogs in ice and water, and subsequently removing the small intestine. For the tests of amylase activity, commercial enzymatic kits were used. Amylase activity was observed since the third day and it increased until reaching stability, when the animals weighed 4.35 g. Amylase activity (137.44 UI) and specific activity (14,688 UI mg-1) remained stable from the seventh day until the end of the experiment.O objetivo deste trabalho foi avaliar a atividade da amilase no quimo da rã-touro, Rana catesbeiana Shaw 1802 (3,6 a 200 g). Oitenta e sete animais foram distribuídos em baias-testes com temperatura e fotoperíodo controlados. As rãs selecionadas na fase pós-metamórfica receberam ração comercial extrusada ad libitum. Durante 87 dias de experimento, foram efetuadas 29 coletas em intervalos de 1 a 8 dias. As coletas do conteúdo intestinal foram feitas mediante a insensibilização das rãs em gelo e água e isolamento posterior do intestino delgado das mesmas. Para os testes da atividade da amilase, foram utilizados kits enzimáticos comerciais. A atividade da amilase foi observada a partir do terceiro dia e apresentou aumento até alcançar estabilidade, quando os animais pesavam 4,35 g. Do sétimo dia até o final do experimento, ocorreu a manutenção da estabilidade da atividade (137,44 UI) e atividade específica da amilase (14.688 UI mg-1).Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraBraga, Luís Gustavo TavaresOliveira, Maria Goreti de AlmeidaLima, William CardosoEuclydes, Ricardo Frederico2005-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7049Pesquisa Agropecuaria Brasileira; v.40, n.10, out. 2005; 947-951Pesquisa Agropecuária Brasileira; v.40, n.10, out. 2005; 947-9511678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7049/4097info:eu-repo/semantics/openAccess2014-09-19T18:27:41Zoai:ojs.seer.sct.embrapa.br:article/7049Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-19T18:27:41Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Amylase activity in the bullfrog during the post-metamorphic phase
Atividade da amilase em rã-touro durante a fase pós-metamórfica
title Amylase activity in the bullfrog during the post-metamorphic phase
spellingShingle Amylase activity in the bullfrog during the post-metamorphic phase
Braga, Luís Gustavo Tavares
Rana catesbeiana; activity enzymatic; digestive enzymes; frogculture
Rana catesbeiana; atividade enzimática; enzimas digestivas; ranicultura
title_short Amylase activity in the bullfrog during the post-metamorphic phase
title_full Amylase activity in the bullfrog during the post-metamorphic phase
title_fullStr Amylase activity in the bullfrog during the post-metamorphic phase
title_full_unstemmed Amylase activity in the bullfrog during the post-metamorphic phase
title_sort Amylase activity in the bullfrog during the post-metamorphic phase
author Braga, Luís Gustavo Tavares
author_facet Braga, Luís Gustavo Tavares
Oliveira, Maria Goreti de Almeida
Lima, William Cardoso
Euclydes, Ricardo Frederico
author_role author
author2 Oliveira, Maria Goreti de Almeida
Lima, William Cardoso
Euclydes, Ricardo Frederico
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Braga, Luís Gustavo Tavares
Oliveira, Maria Goreti de Almeida
Lima, William Cardoso
Euclydes, Ricardo Frederico
dc.subject.por.fl_str_mv Rana catesbeiana; activity enzymatic; digestive enzymes; frogculture
Rana catesbeiana; atividade enzimática; enzimas digestivas; ranicultura
topic Rana catesbeiana; activity enzymatic; digestive enzymes; frogculture
Rana catesbeiana; atividade enzimática; enzimas digestivas; ranicultura
description The objective of this work was to evaluate the amylase activity in the chime of the bullfrog Rana catesbeiana Shaw 1802 (3.6 to 200 g). Eighty-seven animals were distributed in test stalls with controlled temperature and photoperiod. The frogs selected in the post-metamorphic phase received commercial extruded diet ad libitum. During 87 days of the experiment, 29 samples were collected in intervals varying from one to eight days. The intestinal content samples were made by first desensitizing of the frogs in ice and water, and subsequently removing the small intestine. For the tests of amylase activity, commercial enzymatic kits were used. Amylase activity was observed since the third day and it increased until reaching stability, when the animals weighed 4.35 g. Amylase activity (137.44 UI) and specific activity (14,688 UI mg-1) remained stable from the seventh day until the end of the experiment.
publishDate 2005
dc.date.none.fl_str_mv 2005-10-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7049
url https://seer.sct.embrapa.br/index.php/pab/article/view/7049
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7049/4097
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.40, n.10, out. 2005; 947-951
Pesquisa Agropecuária Brasileira; v.40, n.10, out. 2005; 947-951
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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