Boar semen fertility evaluated by a sperm-binding assay to a synthetic substrate
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/6710 |
Resumo: | The objective of this work was to evaluate boar semen fertility by a sperm-binding assay to a synthetic substrate. Motility (MOT) and percentage of bound sperm (PSB) were evaluated after 5, 24, 48 and 72 hours of storage at 17°C. PSB was analyzed in solutions containing 6.25 or 12.5 million of spermatozoa/mL, with or without bovine serum albumin (BSA), processed from two to five ejaculates of four boars. Fifty eight gilts were inseminated, a single time, 24 hours after the beginning of estrus. There was a positive correlation (P = 0.0001; r = 0.33) between MOT and PSB. Higher percentages of PSB were observed with 12.5 million of spermatozoa/mL and in the presence of BSA (P < 0.05). After 72 hours, boar 3 showed lower PSB (P < 0.05) than the other boars. The cleavage (TC) and normal embryo rates did not differ among boars, but boar 3 showed less than 70.0% of TC belonging to the superior quartile while boars 1, 2 and 4 had more than 75.0%. After 24 hours of sperm storage, boars differ in their sperm binding to the synthetic substrate. Binding of swine spermatozoa to the synthetic substrate is higher in the presence of BSA and with the increase of spermatic concentration. |
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Boar semen fertility evaluated by a sperm-binding assay to a synthetic substrateFertilidade de sêmen suíno avaliada pelo teste de ligação dos espermatozóides a um substrato sintéticoserum albumin, artificial insemination, sperm-egg bindingalbumina sérica; inseminação artificial; ligação espermatozóide-ovócitoThe objective of this work was to evaluate boar semen fertility by a sperm-binding assay to a synthetic substrate. Motility (MOT) and percentage of bound sperm (PSB) were evaluated after 5, 24, 48 and 72 hours of storage at 17°C. PSB was analyzed in solutions containing 6.25 or 12.5 million of spermatozoa/mL, with or without bovine serum albumin (BSA), processed from two to five ejaculates of four boars. Fifty eight gilts were inseminated, a single time, 24 hours after the beginning of estrus. There was a positive correlation (P = 0.0001; r = 0.33) between MOT and PSB. Higher percentages of PSB were observed with 12.5 million of spermatozoa/mL and in the presence of BSA (P < 0.05). After 72 hours, boar 3 showed lower PSB (P < 0.05) than the other boars. The cleavage (TC) and normal embryo rates did not differ among boars, but boar 3 showed less than 70.0% of TC belonging to the superior quartile while boars 1, 2 and 4 had more than 75.0%. After 24 hours of sperm storage, boars differ in their sperm binding to the synthetic substrate. Binding of swine spermatozoa to the synthetic substrate is higher in the presence of BSA and with the increase of spermatic concentration.O objetivo deste trabalho foi avaliar a fertilidade de sêmen suíno pelo teste de ligação de espermatozóides a um substrato sintético. A motilidade (MOT) e o porcentual de espermatozóides ligados (PEL) foram avaliados após 5, 24, 48 e 72 horas de armazenamento a 17ºC. O PEL foi determinado em soluções contendo 6,25 ou 12,5 milhões de espermatozóides/mL, com ou sem albumina sérica bovina (BSA), preparadas a partir de dois a cinco ejaculados de cada um dos quatro machos. Cinqüenta e oito leitoas foram inseminadas, uma vez, 24 horas após o início do estro. Houve correlação positiva (P = 0,0001; r = 0,33) entre a MOT e o PEL. O PEL foi maior com 12,5 milhões de espermatozóides/mL e na presença de BSA (P < 0,05). Após 72 horas, o macho 3 apresentou PEL inferior ao dos outros três (P < 0,05). As taxas de clivagem (TC) e de embriões morfologicamente normais não diferiram entre indivíduos, mas o macho 3 apresentou menos de 70,0% de TC no quartil superior, enquanto os outros tiveram mais de 75,0%. Os machos diferem quanto à capacidade de ligação de seus espermatozóides ao substrato sintético, a partir de 24 horas de armazenamento do sêmen. A ligação dos espermatozóides ao substrato sintético é maior com a inclusão de BSA e com o aumento da concentração espermática.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraReis, Goreti Ranincheski dosBernardi, Mari LourdesWentz, IvoBortolozzo, Fernando PandolfoWeitze, Karl FritzAmann, Rupertkellers, ClaudiaZemmrich, Jana2003-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6710Pesquisa Agropecuaria Brasileira; v.38, n.11, nov. 2003; 1343-1349Pesquisa Agropecuária Brasileira; v.38, n.11, nov. 2003; 1343-13491678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/6710/3767info:eu-repo/semantics/openAccess2010-08-20T17:23:54Zoai:ojs.seer.sct.embrapa.br:article/6710Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2010-08-20T17:23:54Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Boar semen fertility evaluated by a sperm-binding assay to a synthetic substrate Fertilidade de sêmen suíno avaliada pelo teste de ligação dos espermatozóides a um substrato sintético |
title |
Boar semen fertility evaluated by a sperm-binding assay to a synthetic substrate |
spellingShingle |
Boar semen fertility evaluated by a sperm-binding assay to a synthetic substrate Reis, Goreti Ranincheski dos serum albumin, artificial insemination, sperm-egg binding albumina sérica; inseminação artificial; ligação espermatozóide-ovócito |
title_short |
Boar semen fertility evaluated by a sperm-binding assay to a synthetic substrate |
title_full |
Boar semen fertility evaluated by a sperm-binding assay to a synthetic substrate |
title_fullStr |
Boar semen fertility evaluated by a sperm-binding assay to a synthetic substrate |
title_full_unstemmed |
Boar semen fertility evaluated by a sperm-binding assay to a synthetic substrate |
title_sort |
Boar semen fertility evaluated by a sperm-binding assay to a synthetic substrate |
author |
Reis, Goreti Ranincheski dos |
author_facet |
Reis, Goreti Ranincheski dos Bernardi, Mari Lourdes Wentz, Ivo Bortolozzo, Fernando Pandolfo Weitze, Karl Fritz Amann, Rupert kellers, Claudia Zemmrich, Jana |
author_role |
author |
author2 |
Bernardi, Mari Lourdes Wentz, Ivo Bortolozzo, Fernando Pandolfo Weitze, Karl Fritz Amann, Rupert kellers, Claudia Zemmrich, Jana |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Reis, Goreti Ranincheski dos Bernardi, Mari Lourdes Wentz, Ivo Bortolozzo, Fernando Pandolfo Weitze, Karl Fritz Amann, Rupert kellers, Claudia Zemmrich, Jana |
dc.subject.por.fl_str_mv |
serum albumin, artificial insemination, sperm-egg binding albumina sérica; inseminação artificial; ligação espermatozóide-ovócito |
topic |
serum albumin, artificial insemination, sperm-egg binding albumina sérica; inseminação artificial; ligação espermatozóide-ovócito |
description |
The objective of this work was to evaluate boar semen fertility by a sperm-binding assay to a synthetic substrate. Motility (MOT) and percentage of bound sperm (PSB) were evaluated after 5, 24, 48 and 72 hours of storage at 17°C. PSB was analyzed in solutions containing 6.25 or 12.5 million of spermatozoa/mL, with or without bovine serum albumin (BSA), processed from two to five ejaculates of four boars. Fifty eight gilts were inseminated, a single time, 24 hours after the beginning of estrus. There was a positive correlation (P = 0.0001; r = 0.33) between MOT and PSB. Higher percentages of PSB were observed with 12.5 million of spermatozoa/mL and in the presence of BSA (P < 0.05). After 72 hours, boar 3 showed lower PSB (P < 0.05) than the other boars. The cleavage (TC) and normal embryo rates did not differ among boars, but boar 3 showed less than 70.0% of TC belonging to the superior quartile while boars 1, 2 and 4 had more than 75.0%. After 24 hours of sperm storage, boars differ in their sperm binding to the synthetic substrate. Binding of swine spermatozoa to the synthetic substrate is higher in the presence of BSA and with the increase of spermatic concentration. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-11-01 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/6710 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/6710 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/6710/3767 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.38, n.11, nov. 2003; 1343-1349 Pesquisa Agropecuária Brasileira; v.38, n.11, nov. 2003; 1343-1349 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416691039862784 |