Sucupira‑preta micropropagation through axillary buds
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/12969 |
Resumo: | The objective of this work was to evaluate the use of growth regulators, antioxidant additives, type of explants, and coverage intensity of acclimatized seedlings in the micropropagation of sucupira‑preta (Bowdichia virgilioides) through axillary buds. Explants were cultured for multiplication on WPM medium, supplemented with concentrations of benzylaminopurine (BAP). In the elongation phase, treatments consisted of naphthaleneacetic acid (NAA) and BAP combinations added to the medium. For rooting, shoots were inoculated in medium containing concentrations of indolbutyric acid or combinations of the additives polyvinylpyrrolidone and charcoal, in different NAA concentrations. Plants were transplanted to plastic cups with substrate, and covered with polyethylene bags. Later, the bags were withdrawn, or perforated, or not withdrawn, which constituted the in vitro pre‑acclimatization treatments. Ex vitro acclimatization was carried out after the period of in vitro pre‑acclimatization. Sucupira‑preta had the best micropropagation using: cotyledon segments and 0.3 mg L-1 BAP, for multiplication; 0.3 mg L-1 NAA and 0.03 mg L-1 BAP, for elongation; and 2,0 mg L-1 activated carbon for rooting and for the in vitro pre‑acclimatization, with the use of a transparent plastic cover around seedling. |
id |
EMBRAPA-4_c915d4436b0bf2564d688b494d6eae8c |
---|---|
oai_identifier_str |
oai:ojs.seer.sct.embrapa.br:article/12969 |
network_acronym_str |
EMBRAPA-4 |
network_name_str |
Pesquisa Agropecuária Brasileira (Online) |
repository_id_str |
|
spelling |
Sucupira‑preta micropropagation through axillary budsMicropropagação de sucupira‑preta por meio de gemas axilaresBowdichia virgilioides; tissue culture; native species; propagation; growth regulatorsBowdichia virgilioides; cultura de tecidos; espécie nativa; propagação; reguladores de crescimentoThe objective of this work was to evaluate the use of growth regulators, antioxidant additives, type of explants, and coverage intensity of acclimatized seedlings in the micropropagation of sucupira‑preta (Bowdichia virgilioides) through axillary buds. Explants were cultured for multiplication on WPM medium, supplemented with concentrations of benzylaminopurine (BAP). In the elongation phase, treatments consisted of naphthaleneacetic acid (NAA) and BAP combinations added to the medium. For rooting, shoots were inoculated in medium containing concentrations of indolbutyric acid or combinations of the additives polyvinylpyrrolidone and charcoal, in different NAA concentrations. Plants were transplanted to plastic cups with substrate, and covered with polyethylene bags. Later, the bags were withdrawn, or perforated, or not withdrawn, which constituted the in vitro pre‑acclimatization treatments. Ex vitro acclimatization was carried out after the period of in vitro pre‑acclimatization. Sucupira‑preta had the best micropropagation using: cotyledon segments and 0.3 mg L-1 BAP, for multiplication; 0.3 mg L-1 NAA and 0.03 mg L-1 BAP, for elongation; and 2,0 mg L-1 activated carbon for rooting and for the in vitro pre‑acclimatization, with the use of a transparent plastic cover around seedling.O objetivo deste trabalho foi avaliar o uso de reguladores de crescimento, aditivos antioxidantes, tipo de explantes e intensidade de cobertura de mudas aclimatizadas, na micropropagação de sucupira‑preta (Bowdichia virgilioides) por meio de gemas axilares. Os explantes foram cultivados para a multiplicação em meio de cultura básico WPM, suplementado com concentrações de benzilaminopurina (BAP). No alongamento, os tratamentos foram combinações de ácido naftalenoacético (ANA) e BAP adicionadas ao meio. Para o enraizamento, as brotações foram colocadas em meio com concentrações de ácido indolbutírico, ou em meio com combinações dos aditivos polivinilpirrolidona e carvão ativado, em diferentes concentrações de ANA. As plantas foram transplantadas para copos de plástico, com substrato, e cobertas com saco de polietileno. Posteriormente, esses sacos foram retirados, ou perfurados, ou não retirados, o que constituiu os tratamentos de pré‑aclimatização in vitro. A aclimatização ex vitro foi realizada após o período de pré‑aclimatização in vitro. A sucupira‑preta apresentou melhor micropropagação com emprego de: segmentos cotiledonares e 0,3 mg L-1 de BAP, na multiplicação; 0,3 mg L-1 de ANA e 0,03 mg L-1 de BAP, no alongamento; e carvão ativado (2,0 mg L-1), no enraizamento e na pré‑aclimatização in vitro, com uso de cobertura de plástico transparente em torno da muda.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraUFVJMFAPEMIGPROCAD/CAPESSECTESIEFMoura, Luciana Coelho deTiton, MirandaFernandes, José Sebastião CunhaSantana, Reynaldo CamposOliveira, Marcio Leles Romarco de2013-01-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/12969Pesquisa Agropecuaria Brasileira; v.47, n.12, dez. 2012; 1691-1698Pesquisa Agropecuária Brasileira; v.47, n.12, dez. 2012; 1691-16981678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/12969/8304https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8682https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8683https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8684https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8685https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8686https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8687https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8688https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8689https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8690info:eu-repo/semantics/openAccess2013-01-22T16:52:55Zoai:ojs.seer.sct.embrapa.br:article/12969Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2013-01-22T16:52:55Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Sucupira‑preta micropropagation through axillary buds Micropropagação de sucupira‑preta por meio de gemas axilares |
title |
Sucupira‑preta micropropagation through axillary buds |
spellingShingle |
Sucupira‑preta micropropagation through axillary buds Moura, Luciana Coelho de Bowdichia virgilioides; tissue culture; native species; propagation; growth regulators Bowdichia virgilioides; cultura de tecidos; espécie nativa; propagação; reguladores de crescimento |
title_short |
Sucupira‑preta micropropagation through axillary buds |
title_full |
Sucupira‑preta micropropagation through axillary buds |
title_fullStr |
Sucupira‑preta micropropagation through axillary buds |
title_full_unstemmed |
Sucupira‑preta micropropagation through axillary buds |
title_sort |
Sucupira‑preta micropropagation through axillary buds |
author |
Moura, Luciana Coelho de |
author_facet |
Moura, Luciana Coelho de Titon, Miranda Fernandes, José Sebastião Cunha Santana, Reynaldo Campos Oliveira, Marcio Leles Romarco de |
author_role |
author |
author2 |
Titon, Miranda Fernandes, José Sebastião Cunha Santana, Reynaldo Campos Oliveira, Marcio Leles Romarco de |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
UFVJM FAPEMIG PROCAD/CAPES SECTES IEF |
dc.contributor.author.fl_str_mv |
Moura, Luciana Coelho de Titon, Miranda Fernandes, José Sebastião Cunha Santana, Reynaldo Campos Oliveira, Marcio Leles Romarco de |
dc.subject.por.fl_str_mv |
Bowdichia virgilioides; tissue culture; native species; propagation; growth regulators Bowdichia virgilioides; cultura de tecidos; espécie nativa; propagação; reguladores de crescimento |
topic |
Bowdichia virgilioides; tissue culture; native species; propagation; growth regulators Bowdichia virgilioides; cultura de tecidos; espécie nativa; propagação; reguladores de crescimento |
description |
The objective of this work was to evaluate the use of growth regulators, antioxidant additives, type of explants, and coverage intensity of acclimatized seedlings in the micropropagation of sucupira‑preta (Bowdichia virgilioides) through axillary buds. Explants were cultured for multiplication on WPM medium, supplemented with concentrations of benzylaminopurine (BAP). In the elongation phase, treatments consisted of naphthaleneacetic acid (NAA) and BAP combinations added to the medium. For rooting, shoots were inoculated in medium containing concentrations of indolbutyric acid or combinations of the additives polyvinylpyrrolidone and charcoal, in different NAA concentrations. Plants were transplanted to plastic cups with substrate, and covered with polyethylene bags. Later, the bags were withdrawn, or perforated, or not withdrawn, which constituted the in vitro pre‑acclimatization treatments. Ex vitro acclimatization was carried out after the period of in vitro pre‑acclimatization. Sucupira‑preta had the best micropropagation using: cotyledon segments and 0.3 mg L-1 BAP, for multiplication; 0.3 mg L-1 NAA and 0.03 mg L-1 BAP, for elongation; and 2,0 mg L-1 activated carbon for rooting and for the in vitro pre‑acclimatization, with the use of a transparent plastic cover around seedling. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-22 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/12969 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/12969 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/12969/8304 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8682 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8683 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8684 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8685 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8686 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8687 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8688 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8689 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12969/8690 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.47, n.12, dez. 2012; 1691-1698 Pesquisa Agropecuária Brasileira; v.47, n.12, dez. 2012; 1691-1698 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
_version_ |
1793416703230607360 |