DNA index and anatomical aspects of the micrografting of dragon fruit on different rootstocks

Detalhes bibliográficos
Autor(a) principal: Moreira, Renata Amato
Data de Publicação: 2022
Outros Autores: Rodrigues, Mariane Aparecida, Magalhães, Deniete Soares, Pio, Leila Aparecida Salles, Santos, Dalilhia Nazaré dos, Guimarães, Paulo Henrique Sales, Ramos, José Darlan, Pascoal, Moacir
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/26998
Resumo: The objective of this work was to evaluate the viability of the micrografting of yellow dragon fruit (Selenicereus megalanthus) on different rootstocks, based on DNA content and anatomical analyses. The used rootstocks were: yellow dragon fruit, white dragon fruit (Hylocereus undatus), Saborosa (Selenicereus setaceus) dragon fruit, and the Cebra and Orejona red dragon fruit (Hylocereus polyrhizus) varieties. The experimental design was completely randomized with five treatments and four replicates of five plants. After 30 days of cultivation, the following traits were evaluated: length and diameter of the micrografts and microrootstocks; and root length, percentage of setting, and fresh mass of the micrografts. Flow cytometry analyzes were performed before and after micrografting to verify genetic stability and the occurrence of endoreduplication. In addition, histological sections were made in the micrografting region to verify the connections of vessels and tissues between the graft and the rootstock. Endoreduplication was observed in all treatments. The amount of DNA in the yellow dragon fruit micrograft increased on the red Orejona variety. The presence of vessel connections was verified between the micrografts and microrootstocks. The yellow dragon fruit was also more vigorous when grafted on Orejona. Based on DNA content and anatomical analyses, in vitro yellow dragon fruit micrografting is feasible in all used rootstocks.
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spelling DNA index and anatomical aspects of the micrografting of dragon fruit on different rootstocksÍndice de DNA e aspectos anatômicos da microenxertia de pitaia em diferentes porta-enxertosHylocereus polyrhizus; Hylocereus undatus; pitaya; propagation; scionHylocereus polyrhizus; Hylocereus undatus; pitaya; propagação; enxertoThe objective of this work was to evaluate the viability of the micrografting of yellow dragon fruit (Selenicereus megalanthus) on different rootstocks, based on DNA content and anatomical analyses. The used rootstocks were: yellow dragon fruit, white dragon fruit (Hylocereus undatus), Saborosa (Selenicereus setaceus) dragon fruit, and the Cebra and Orejona red dragon fruit (Hylocereus polyrhizus) varieties. The experimental design was completely randomized with five treatments and four replicates of five plants. After 30 days of cultivation, the following traits were evaluated: length and diameter of the micrografts and microrootstocks; and root length, percentage of setting, and fresh mass of the micrografts. Flow cytometry analyzes were performed before and after micrografting to verify genetic stability and the occurrence of endoreduplication. In addition, histological sections were made in the micrografting region to verify the connections of vessels and tissues between the graft and the rootstock. Endoreduplication was observed in all treatments. The amount of DNA in the yellow dragon fruit micrograft increased on the red Orejona variety. The presence of vessel connections was verified between the micrografts and microrootstocks. The yellow dragon fruit was also more vigorous when grafted on Orejona. Based on DNA content and anatomical analyses, in vitro yellow dragon fruit micrografting is feasible in all used rootstocks.O objetivo deste trabalho foi avaliar a viabilidade da microenxertia de pitaia amarela (Selenicereus megalanthus) sobre diferentes microporta-enxertos, com base em análises de conteúdo de DNA e anatômicas. Os porta-enxertos utilizados foram: pitaia amarela, pitaia branca (Hylocereus undatus), pitaia Saborosa (Selenicereus setaceus), e as variedades Cebra e Orejona de pitaia vermelha (Hylocereus polyrhizus). O delineamento experimental foi inteiramente casualizado, com cinco tratamentos e quatro repetições de cinco plantas. Após 30 dias de cultivo, avaliaram-se as seguintes características: comprimento e diâmetro dos microenxertos e dos microporta-enxertos; e comprimento de raízes, percentagem de pegamento e massa fresca dos microenxertos. Realizaram-se análises de citometria de fluxo antes e depois da microenxertia, para verificação da estabilidade genética e da ocorrência de endoreduplicação. Além disso, foram feitos cortes histológicos na região da microenxertia, para verificação das conexões dos vasos e dos tecidos entre o enxerto e o porta-enxerto. Observou-se endoreduplicação em todos os tratamentos. A quantidade de DNA do microenxerto de pitaia amarela aumentou sobre a variedade vermelha Orejona. Verificou-se a presença de conexões de vasos entre os microenxertos e os microporta-enxertos. A pitaia amarela também foi mais vigorosa quando enxertada sobre Orejona. Com base nas análises de conteúdo de DNA e anatômicas, a microenxertia de pitaia amarela in vitro é viável em todos os porta-enxertos utilizados.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraConselho Nacional de Desenvolvimento Científico e TecnológicoConselho Nacional de Desenvolvimento Científico e TecnológicoMoreira, Renata AmatoRodrigues, Mariane AparecidaMagalhães, Deniete SoaresPio, Leila Aparecida SallesSantos, Dalilhia Nazaré dosGuimarães, Paulo Henrique SalesRamos, José DarlanPascoal, Moacir2022-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/26998Pesquisa Agropecuaria Brasileira; V.56, Jan./Dec., 2021: Publicação contínua em volume anual; e01867Pesquisa Agropecuária Brasileira; V.56, Jan./Dec., 2021: Publicação contínua em volume anual; e018671678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/26998/14943Direitos autorais 2022 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2022-05-18T18:27:35Zoai:ojs.seer.sct.embrapa.br:article/26998Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2022-05-18T18:27:35Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv DNA index and anatomical aspects of the micrografting of dragon fruit on different rootstocks
Índice de DNA e aspectos anatômicos da microenxertia de pitaia em diferentes porta-enxertos
title DNA index and anatomical aspects of the micrografting of dragon fruit on different rootstocks
spellingShingle DNA index and anatomical aspects of the micrografting of dragon fruit on different rootstocks
Moreira, Renata Amato
Hylocereus polyrhizus; Hylocereus undatus; pitaya; propagation; scion
Hylocereus polyrhizus; Hylocereus undatus; pitaya; propagação; enxerto
title_short DNA index and anatomical aspects of the micrografting of dragon fruit on different rootstocks
title_full DNA index and anatomical aspects of the micrografting of dragon fruit on different rootstocks
title_fullStr DNA index and anatomical aspects of the micrografting of dragon fruit on different rootstocks
title_full_unstemmed DNA index and anatomical aspects of the micrografting of dragon fruit on different rootstocks
title_sort DNA index and anatomical aspects of the micrografting of dragon fruit on different rootstocks
author Moreira, Renata Amato
author_facet Moreira, Renata Amato
Rodrigues, Mariane Aparecida
Magalhães, Deniete Soares
Pio, Leila Aparecida Salles
Santos, Dalilhia Nazaré dos
Guimarães, Paulo Henrique Sales
Ramos, José Darlan
Pascoal, Moacir
author_role author
author2 Rodrigues, Mariane Aparecida
Magalhães, Deniete Soares
Pio, Leila Aparecida Salles
Santos, Dalilhia Nazaré dos
Guimarães, Paulo Henrique Sales
Ramos, José Darlan
Pascoal, Moacir
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Conselho Nacional de Desenvolvimento Científico e Tecnológico
Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.contributor.author.fl_str_mv Moreira, Renata Amato
Rodrigues, Mariane Aparecida
Magalhães, Deniete Soares
Pio, Leila Aparecida Salles
Santos, Dalilhia Nazaré dos
Guimarães, Paulo Henrique Sales
Ramos, José Darlan
Pascoal, Moacir
dc.subject.por.fl_str_mv Hylocereus polyrhizus; Hylocereus undatus; pitaya; propagation; scion
Hylocereus polyrhizus; Hylocereus undatus; pitaya; propagação; enxerto
topic Hylocereus polyrhizus; Hylocereus undatus; pitaya; propagation; scion
Hylocereus polyrhizus; Hylocereus undatus; pitaya; propagação; enxerto
description The objective of this work was to evaluate the viability of the micrografting of yellow dragon fruit (Selenicereus megalanthus) on different rootstocks, based on DNA content and anatomical analyses. The used rootstocks were: yellow dragon fruit, white dragon fruit (Hylocereus undatus), Saborosa (Selenicereus setaceus) dragon fruit, and the Cebra and Orejona red dragon fruit (Hylocereus polyrhizus) varieties. The experimental design was completely randomized with five treatments and four replicates of five plants. After 30 days of cultivation, the following traits were evaluated: length and diameter of the micrografts and microrootstocks; and root length, percentage of setting, and fresh mass of the micrografts. Flow cytometry analyzes were performed before and after micrografting to verify genetic stability and the occurrence of endoreduplication. In addition, histological sections were made in the micrografting region to verify the connections of vessels and tissues between the graft and the rootstock. Endoreduplication was observed in all treatments. The amount of DNA in the yellow dragon fruit micrograft increased on the red Orejona variety. The presence of vessel connections was verified between the micrografts and microrootstocks. The yellow dragon fruit was also more vigorous when grafted on Orejona. Based on DNA content and anatomical analyses, in vitro yellow dragon fruit micrografting is feasible in all used rootstocks.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-13
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/26998
url https://seer.sct.embrapa.br/index.php/pab/article/view/26998
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/26998/14943
dc.rights.driver.fl_str_mv Direitos autorais 2022 Pesquisa Agropecuária Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2022 Pesquisa Agropecuária Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; V.56, Jan./Dec., 2021: Publicação contínua em volume anual; e01867
Pesquisa Agropecuária Brasileira; V.56, Jan./Dec., 2021: Publicação contínua em volume anual; e01867
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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