Resistance genetic basis of a wild tomato access to pepper yellow mosaic virus
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/108 |
Resumo: | The aim of this work was to evaluate the genetic basis of resistance of Lycopersicon hirsutum to the potyvirus Pepper yellow mosaic virus (PepYMV). Five hundred and forty plants, including the parentals 'Santa Clara' (susceptible) and 'BGH 6902' (resistant) were evaluated, as well as the generations F1, F2, RC1:1 and RC1:2, from the crossing of the above parentals. PepYMV was mechanically inoculated, and the virus concentration in each plant was determined by indirect ELISA. The quantitative and qualitative analyses were carried out. The first one, based on the virus concentration of each plant, suggest oligogenic inheritance with heritability of 99%. The same data, when analyzed under the qualitative form, indicated an inheritance governed by two genes, with dominant and recessive epistatic interaction. However, when F2:3 generation, arising from self-fertilization of resistant F2 plants, was analyzed, the hypothesis of two genes was rejected, while the best fitted to data was that of one gene with complete dominance among the alleles. The qualitative analysis, considering sintomatology observed, showed that the inheritance of resistance to PepYMV is determined by a recessive gene, with the absence of dominance among their alleles. |
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Resistance genetic basis of a wild tomato access to pepper yellow mosaic virusBase genética da resistência de um acesso de tomate silvestre ao mosaico-amarelo do pimentãoLycopersicon hirsutum; Pepper yellow mosaic virus; inheritance of resistance; recessive resistanceLycopersicon hirsutum; Pepper yellow mosaic virus; herança da resistência; resistência recessivaThe aim of this work was to evaluate the genetic basis of resistance of Lycopersicon hirsutum to the potyvirus Pepper yellow mosaic virus (PepYMV). Five hundred and forty plants, including the parentals 'Santa Clara' (susceptible) and 'BGH 6902' (resistant) were evaluated, as well as the generations F1, F2, RC1:1 and RC1:2, from the crossing of the above parentals. PepYMV was mechanically inoculated, and the virus concentration in each plant was determined by indirect ELISA. The quantitative and qualitative analyses were carried out. The first one, based on the virus concentration of each plant, suggest oligogenic inheritance with heritability of 99%. The same data, when analyzed under the qualitative form, indicated an inheritance governed by two genes, with dominant and recessive epistatic interaction. However, when F2:3 generation, arising from self-fertilization of resistant F2 plants, was analyzed, the hypothesis of two genes was rejected, while the best fitted to data was that of one gene with complete dominance among the alleles. The qualitative analysis, considering sintomatology observed, showed that the inheritance of resistance to PepYMV is determined by a recessive gene, with the absence of dominance among their alleles.O objetivo deste trabalho foi avaliar a base genética da resistência de Lycopersicon hirsutum ao potyvírus Pepper yellow mosaic virus (PepYMV). Foram avaliadas 540 plantas, inclusive os parentais 'Santa Clara' (suscetível) e 'BGH 6902' (resistente), e as gerações F1, F2, RC1:1 e RC1:2, derivadas do cruzamento desses parentais. As plantas receberam inoculações mecanicamente, e a concentração viral de PepYMV em cada planta foi determinada por ELISA indireto. Foram realizadas as análises quantitativa e qualitativa. A primeira, baseada na concentração viral de cada planta, indicou herança oligogênica com herdabilidade de 99%. Os mesmos dados, quando analisados de forma qualitativa, indicaram herança governada por dois genes, com interação epistática dominante e recessiva. Entretanto, quando foi analisada a geração F2:3, oriunda da autofecundação de plantas F2 resistentes, a hipótese de dois genes foi descartada e a de um gene, com dominância completa entre os alelos, foi a que melhor se ajustou aos dados. A análise qualitativa, pela sintomatologia observada, demonstrou que a herança da resistência ao PepYMV é determinada por um gene recessivo, com ausência de dominância entre os seus alelos.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Juhász, Ana Cristina PintoSilva, Derly José Henriques daJúnior, Francisco Murilo ZerbiniCarneiro, Pedro Crescêncio SouzaSoares, Bruno OliveiraCruz, Cosme Damião2008-10-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/108Pesquisa Agropecuaria Brasileira; v.43, n.6, jun. 2008; 713-720Pesquisa Agropecuária Brasileira; v.43, n.6, jun. 2008; 713-7201678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/108/5448https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/108/82https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/108/83https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/108/460info:eu-repo/semantics/openAccess2014-07-02T17:50:31Zoai:ojs.seer.sct.embrapa.br:article/108Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-02T17:50:31Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Resistance genetic basis of a wild tomato access to pepper yellow mosaic virus Base genética da resistência de um acesso de tomate silvestre ao mosaico-amarelo do pimentão |
title |
Resistance genetic basis of a wild tomato access to pepper yellow mosaic virus |
spellingShingle |
Resistance genetic basis of a wild tomato access to pepper yellow mosaic virus Juhász, Ana Cristina Pinto Lycopersicon hirsutum; Pepper yellow mosaic virus; inheritance of resistance; recessive resistance Lycopersicon hirsutum; Pepper yellow mosaic virus; herança da resistência; resistência recessiva |
title_short |
Resistance genetic basis of a wild tomato access to pepper yellow mosaic virus |
title_full |
Resistance genetic basis of a wild tomato access to pepper yellow mosaic virus |
title_fullStr |
Resistance genetic basis of a wild tomato access to pepper yellow mosaic virus |
title_full_unstemmed |
Resistance genetic basis of a wild tomato access to pepper yellow mosaic virus |
title_sort |
Resistance genetic basis of a wild tomato access to pepper yellow mosaic virus |
author |
Juhász, Ana Cristina Pinto |
author_facet |
Juhász, Ana Cristina Pinto Silva, Derly José Henriques da Júnior, Francisco Murilo Zerbini Carneiro, Pedro Crescêncio Souza Soares, Bruno Oliveira Cruz, Cosme Damião |
author_role |
author |
author2 |
Silva, Derly José Henriques da Júnior, Francisco Murilo Zerbini Carneiro, Pedro Crescêncio Souza Soares, Bruno Oliveira Cruz, Cosme Damião |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) |
dc.contributor.author.fl_str_mv |
Juhász, Ana Cristina Pinto Silva, Derly José Henriques da Júnior, Francisco Murilo Zerbini Carneiro, Pedro Crescêncio Souza Soares, Bruno Oliveira Cruz, Cosme Damião |
dc.subject.por.fl_str_mv |
Lycopersicon hirsutum; Pepper yellow mosaic virus; inheritance of resistance; recessive resistance Lycopersicon hirsutum; Pepper yellow mosaic virus; herança da resistência; resistência recessiva |
topic |
Lycopersicon hirsutum; Pepper yellow mosaic virus; inheritance of resistance; recessive resistance Lycopersicon hirsutum; Pepper yellow mosaic virus; herança da resistência; resistência recessiva |
description |
The aim of this work was to evaluate the genetic basis of resistance of Lycopersicon hirsutum to the potyvirus Pepper yellow mosaic virus (PepYMV). Five hundred and forty plants, including the parentals 'Santa Clara' (susceptible) and 'BGH 6902' (resistant) were evaluated, as well as the generations F1, F2, RC1:1 and RC1:2, from the crossing of the above parentals. PepYMV was mechanically inoculated, and the virus concentration in each plant was determined by indirect ELISA. The quantitative and qualitative analyses were carried out. The first one, based on the virus concentration of each plant, suggest oligogenic inheritance with heritability of 99%. The same data, when analyzed under the qualitative form, indicated an inheritance governed by two genes, with dominant and recessive epistatic interaction. However, when F2:3 generation, arising from self-fertilization of resistant F2 plants, was analyzed, the hypothesis of two genes was rejected, while the best fitted to data was that of one gene with complete dominance among the alleles. The qualitative analysis, considering sintomatology observed, showed that the inheritance of resistance to PepYMV is determined by a recessive gene, with the absence of dominance among their alleles. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10-20 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/108 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/108 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/108/5448 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/108/82 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/108/83 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/108/460 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.43, n.6, jun. 2008; 713-720 Pesquisa Agropecuária Brasileira; v.43, n.6, jun. 2008; 713-720 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416665699975168 |