Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
| Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/26630 |
Resumo: | The objective of this work was to sequence the MyoD1, MyoG, MyF5, MyF6, and MSTN genes and to identify polymorphisms in Santa Inês sheep (Ovis aries). A total of 192 lambs with 240 days of age were evaluated, and these genes were sequenced to be compared with the reference sequence in the Ovis aries genome. Genotype and allele frequencies were estimated, and the Hardy-Weinberg equilibrium was tested. Fragments containing 2,493 bp (MyoD1), 1,836 bp (MyoG), 2,813 bp (MyF5), 1,126 bp (MyF6), and 2,380 bp (MSTN) were obtained, and, in these sequences, 160 variants were identified. These polymorphisms were distributed as follows: 59 (MyoD1), 24 (MyoG), 63 (MyF5), 4 (MyF6), and 10 (MSTN). One hundred and four were novel polymorphisms, 45 in MyoD1, 2 in MyoG, 56 in MyF5, and 1 in MSTN. Regarding site, 61 were in intron (27 in MyoD1, 16 in MyoG, 5 in MyF5, 3 in MyF6, and 10 in MSTN), 87 in coding region (22 in MyoD1, 8 in MyoG, 56 in MyF5, and 1 in MyF6), and 12 on 3’UTR (10 in MyoD1 and 2 in MyF5). Therefore, the MyoD family and MSTN genes have several polymorphisms in Santa Inês sheep, which can be useful for association studies. |
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Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheepPolimorfismos nos genes MyoD1, MyoG, MyF5, MyF6 e MSTN em ovinos Santa InêsOvis aries; candidate genes; frequencies; variantsOvis aries; genes candidatos; frequências; variantesThe objective of this work was to sequence the MyoD1, MyoG, MyF5, MyF6, and MSTN genes and to identify polymorphisms in Santa Inês sheep (Ovis aries). A total of 192 lambs with 240 days of age were evaluated, and these genes were sequenced to be compared with the reference sequence in the Ovis aries genome. Genotype and allele frequencies were estimated, and the Hardy-Weinberg equilibrium was tested. Fragments containing 2,493 bp (MyoD1), 1,836 bp (MyoG), 2,813 bp (MyF5), 1,126 bp (MyF6), and 2,380 bp (MSTN) were obtained, and, in these sequences, 160 variants were identified. These polymorphisms were distributed as follows: 59 (MyoD1), 24 (MyoG), 63 (MyF5), 4 (MyF6), and 10 (MSTN). One hundred and four were novel polymorphisms, 45 in MyoD1, 2 in MyoG, 56 in MyF5, and 1 in MSTN. Regarding site, 61 were in intron (27 in MyoD1, 16 in MyoG, 5 in MyF5, 3 in MyF6, and 10 in MSTN), 87 in coding region (22 in MyoD1, 8 in MyoG, 56 in MyF5, and 1 in MyF6), and 12 on 3’UTR (10 in MyoD1 and 2 in MyF5). Therefore, the MyoD family and MSTN genes have several polymorphisms in Santa Inês sheep, which can be useful for association studies.O objetivo deste trabalho foi sequenciar os genes MyoD1, MyoG, MyF5, MyF6 e MSTN e identificar polimorfismos em ovinos Santa Inês (Ovis aries). No total, 192 cordeiros com 240 dias de idade foram avaliados, e estes genes foram sequenciados para comparação com a sequência-referência no genoma de Ovis aries. As frequências genotípicas e alélicas foram estimadas, e o equilíbrio de Hardy-Weinberg, testado. Foram obtidos fragmentos contendo 2.493 pb (MyoDl), 1.836 pb (MyoG), 2.813 pb (MyF5), 1.126 pb (MyF6) e 2.380 pb (MSTN), e, nessas sequências, foram identificadas 160 variantes. Esses polimorfismos foram distribuídos da seguinte forma: 59 (MyoD1), 24 (MyoG), 63 (MyF5), 4 (MyF6) e 10 (MSTN). Foram encontrados 104 novos polimorfismos, sendo 45 no MyoD1, 2 no MyoG, 56 no MyF5 e 1 no MSTN. Com relação ao local, 61 variantes estavam em íntron (27 no MyoD1, 16 no MyoG, 5 no MyF5, 3 no MyF6 e 10 no MSTN), 87 em região codificante (22 no MyoD1, 8 no MyoG, 56 no MyF5 e 1 no MyF6) e 12 na região 3’UTR (10 no MyoD1 e 2 no MyF5). Portanto, os genes da família MyoD e o MSTN possuem vários polimorfismos em ovinos da raça Santa Inês, os quais podem ser úteis em estudos de associação.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFundação de Amparo à Pesquisa da Bahia (Fapesb)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes)Sousa Junior, Luis Paulo BatistaMeira, Ariana NascimentoAzevedo, Hymerson CostaMuniz, Evandro NevezCoutinho, Luiz LehmannMourão, Gerson BarretoPedrosa, Victor BrenoPinto, Luís Fernando Batista2019-11-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/26630Pesquisa Agropecuaria Brasileira; V.54, Jan./Dec., 2019: Publicação contínua em volume anual; e01132Pesquisa Agropecuária Brasileira; V.54, Jan./Dec., 2019: Publicação contínua em volume anual; e011321678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/26630/14545Direitos autorais 2019 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2019-12-11T14:17:48Zoai:ojs.seer.sct.embrapa.br:article/26630Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2019-12-11T14:17:48Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
| dc.title.none.fl_str_mv |
Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep Polimorfismos nos genes MyoD1, MyoG, MyF5, MyF6 e MSTN em ovinos Santa Inês |
| title |
Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep |
| spellingShingle |
Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep Sousa Junior, Luis Paulo Batista Ovis aries; candidate genes; frequencies; variants Ovis aries; genes candidatos; frequências; variantes |
| title_short |
Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep |
| title_full |
Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep |
| title_fullStr |
Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep |
| title_full_unstemmed |
Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep |
| title_sort |
Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep |
| author |
Sousa Junior, Luis Paulo Batista |
| author_facet |
Sousa Junior, Luis Paulo Batista Meira, Ariana Nascimento Azevedo, Hymerson Costa Muniz, Evandro Nevez Coutinho, Luiz Lehmann Mourão, Gerson Barreto Pedrosa, Victor Breno Pinto, Luís Fernando Batista |
| author_role |
author |
| author2 |
Meira, Ariana Nascimento Azevedo, Hymerson Costa Muniz, Evandro Nevez Coutinho, Luiz Lehmann Mourão, Gerson Barreto Pedrosa, Victor Breno Pinto, Luís Fernando Batista |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Fundação de Amparo à Pesquisa da Bahia (Fapesb) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes) |
| dc.contributor.author.fl_str_mv |
Sousa Junior, Luis Paulo Batista Meira, Ariana Nascimento Azevedo, Hymerson Costa Muniz, Evandro Nevez Coutinho, Luiz Lehmann Mourão, Gerson Barreto Pedrosa, Victor Breno Pinto, Luís Fernando Batista |
| dc.subject.por.fl_str_mv |
Ovis aries; candidate genes; frequencies; variants Ovis aries; genes candidatos; frequências; variantes |
| topic |
Ovis aries; candidate genes; frequencies; variants Ovis aries; genes candidatos; frequências; variantes |
| description |
The objective of this work was to sequence the MyoD1, MyoG, MyF5, MyF6, and MSTN genes and to identify polymorphisms in Santa Inês sheep (Ovis aries). A total of 192 lambs with 240 days of age were evaluated, and these genes were sequenced to be compared with the reference sequence in the Ovis aries genome. Genotype and allele frequencies were estimated, and the Hardy-Weinberg equilibrium was tested. Fragments containing 2,493 bp (MyoD1), 1,836 bp (MyoG), 2,813 bp (MyF5), 1,126 bp (MyF6), and 2,380 bp (MSTN) were obtained, and, in these sequences, 160 variants were identified. These polymorphisms were distributed as follows: 59 (MyoD1), 24 (MyoG), 63 (MyF5), 4 (MyF6), and 10 (MSTN). One hundred and four were novel polymorphisms, 45 in MyoD1, 2 in MyoG, 56 in MyF5, and 1 in MSTN. Regarding site, 61 were in intron (27 in MyoD1, 16 in MyoG, 5 in MyF5, 3 in MyF6, and 10 in MSTN), 87 in coding region (22 in MyoD1, 8 in MyoG, 56 in MyF5, and 1 in MyF6), and 12 on 3’UTR (10 in MyoD1 and 2 in MyF5). Therefore, the MyoD family and MSTN genes have several polymorphisms in Santa Inês sheep, which can be useful for association studies. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-11-19 |
| dc.type.none.fl_str_mv |
|
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://seer.sct.embrapa.br/index.php/pab/article/view/26630 |
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https://seer.sct.embrapa.br/index.php/pab/article/view/26630 |
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eng |
| language |
eng |
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https://seer.sct.embrapa.br/index.php/pab/article/view/26630/14545 |
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Direitos autorais 2019 Pesquisa Agropecuária Brasileira info:eu-repo/semantics/openAccess |
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Direitos autorais 2019 Pesquisa Agropecuária Brasileira |
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openAccess |
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application/pdf |
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Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
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Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
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Pesquisa Agropecuaria Brasileira; V.54, Jan./Dec., 2019: Publicação contínua em volume anual; e01132 Pesquisa Agropecuária Brasileira; V.54, Jan./Dec., 2019: Publicação contínua em volume anual; e01132 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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