HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106259 https://doi.org/10.1590/rbz4720160390 |
Resumo: | The purpose of this study was to access and characterize single nucleotide polymorphisms (SNP) located within the HSF1 and HSPA6 candidate genes for adaptability in Angus breed raised in subtropical climate. Samples of DNA from 20 animals representing extreme phenotypes for adaptability traits were obtained. Sequence variations in the candidate genes were described by sequencing target regions. We identified 12 SNP located in the HSF1 gene. Moreover, four of the six SNP found in the HSPA6 gene cause amino acid substitutions in protein-coding regions. We also identified a representative SNP (called tag SNP) in a region of the HSF1 gene with high linkage disequilibrium (r2 = 0.87) that may represent 11 SNP located in this gene. Minor allele frequency observed for the SNP ranged from 0.10 to 0.50 and 0.02 to 0.21 for the HSF1 and the HSPA6 genes, respectively. Overall, almost all SNP analyzed showed significant deviation from the Hardy-Weinberg Equilibrium and half of the loci had heterozygosity greater than 0.50. The data suggest that there is sequence variability in these genes that could be exploited by breeding programs. There is genetic variation in HSF1 and HSPA6 genes in this populations of Angus breed, which is fundamental to obtain response to selection. |
id |
EMBR_04fae914b29ea65bcb53aa46d3348a37 |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1106259 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle.Molecular makersRaça AngusGado de CorteBovinoAngusThermoregulationBeef cattleThe purpose of this study was to access and characterize single nucleotide polymorphisms (SNP) located within the HSF1 and HSPA6 candidate genes for adaptability in Angus breed raised in subtropical climate. Samples of DNA from 20 animals representing extreme phenotypes for adaptability traits were obtained. Sequence variations in the candidate genes were described by sequencing target regions. We identified 12 SNP located in the HSF1 gene. Moreover, four of the six SNP found in the HSPA6 gene cause amino acid substitutions in protein-coding regions. We also identified a representative SNP (called tag SNP) in a region of the HSF1 gene with high linkage disequilibrium (r2 = 0.87) that may represent 11 SNP located in this gene. Minor allele frequency observed for the SNP ranged from 0.10 to 0.50 and 0.02 to 0.21 for the HSF1 and the HSPA6 genes, respectively. Overall, almost all SNP analyzed showed significant deviation from the Hardy-Weinberg Equilibrium and half of the loci had heterozygosity greater than 0.50. The data suggest that there is sequence variability in these genes that could be exploited by breeding programs. There is genetic variation in HSF1 and HSPA6 genes in this populations of Angus breed, which is fundamental to obtain response to selection.Marielle Moura Baena, UFLA; Polyana Cristiane Tizioto, UFSCAR; Sarah Laguna Conceição Meirelles, UFLA; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.BAENA, M. M.TIZIOTO, P. C.MEIRELLES, S. L. C.REGITANO, L. C. de A.2019-02-20T00:36:33Z2019-02-20T00:36:33Z2019-02-1920182019-02-20T00:36:33Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Zootecnia, v.47,e20160390, p. 2-7, 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106259https://doi.org/10.1590/rbz4720160390enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-02-20T00:36:40Zoai:www.alice.cnptia.embrapa.br:doc/1106259Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-02-20T00:36:40falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-02-20T00:36:40Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle. |
title |
HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle. |
spellingShingle |
HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle. BAENA, M. M. Molecular makers Raça Angus Gado de Corte Bovino Angus Thermoregulation Beef cattle |
title_short |
HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle. |
title_full |
HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle. |
title_fullStr |
HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle. |
title_full_unstemmed |
HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle. |
title_sort |
HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle. |
author |
BAENA, M. M. |
author_facet |
BAENA, M. M. TIZIOTO, P. C. MEIRELLES, S. L. C. REGITANO, L. C. de A. |
author_role |
author |
author2 |
TIZIOTO, P. C. MEIRELLES, S. L. C. REGITANO, L. C. de A. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Marielle Moura Baena, UFLA; Polyana Cristiane Tizioto, UFSCAR; Sarah Laguna Conceição Meirelles, UFLA; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
dc.contributor.author.fl_str_mv |
BAENA, M. M. TIZIOTO, P. C. MEIRELLES, S. L. C. REGITANO, L. C. de A. |
dc.subject.por.fl_str_mv |
Molecular makers Raça Angus Gado de Corte Bovino Angus Thermoregulation Beef cattle |
topic |
Molecular makers Raça Angus Gado de Corte Bovino Angus Thermoregulation Beef cattle |
description |
The purpose of this study was to access and characterize single nucleotide polymorphisms (SNP) located within the HSF1 and HSPA6 candidate genes for adaptability in Angus breed raised in subtropical climate. Samples of DNA from 20 animals representing extreme phenotypes for adaptability traits were obtained. Sequence variations in the candidate genes were described by sequencing target regions. We identified 12 SNP located in the HSF1 gene. Moreover, four of the six SNP found in the HSPA6 gene cause amino acid substitutions in protein-coding regions. We also identified a representative SNP (called tag SNP) in a region of the HSF1 gene with high linkage disequilibrium (r2 = 0.87) that may represent 11 SNP located in this gene. Minor allele frequency observed for the SNP ranged from 0.10 to 0.50 and 0.02 to 0.21 for the HSF1 and the HSPA6 genes, respectively. Overall, almost all SNP analyzed showed significant deviation from the Hardy-Weinberg Equilibrium and half of the loci had heterozygosity greater than 0.50. The data suggest that there is sequence variability in these genes that could be exploited by breeding programs. There is genetic variation in HSF1 and HSPA6 genes in this populations of Angus breed, which is fundamental to obtain response to selection. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2019-02-20T00:36:33Z 2019-02-20T00:36:33Z 2019-02-19 2019-02-20T00:36:33Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Zootecnia, v.47,e20160390, p. 2-7, 2018. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106259 https://doi.org/10.1590/rbz4720160390 |
identifier_str_mv |
Revista Brasileira de Zootecnia, v.47,e20160390, p. 2-7, 2018. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106259 https://doi.org/10.1590/rbz4720160390 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503471584509952 |