Vertical transmissibility of small ruminant lentivirus.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127175 https://doi.org/10.1371/journal.pone.0239916 |
Resumo: | Abstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats. |
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Vertical transmissibility of small ruminant lentivirus.SRLVGenetic techniques and protocolsLentivirusSheep diseasesGoat diseasesGoatsAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats.JUSCILÂNIA FURTADO ARAÚJO; ALICE ANDRIOLI, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; LUCIA HELENA SIDER, CNPC; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO; RENATO MESQUITA PEIXOTO; ANA MILENA CESAR LIMA; EDGAR MARQUES DAMASCENO; SAMARA CRISTINA ROCHA SOUZA; MARIA FÁTIMA DA SILVA TEIXEIRA.ARAÚJO, J. F.ANDRIOLI, A.PINHEIRO, R. R.SIDER, L. H.SOUSA, A. L. M. deAZEVEDO, D. A. A. dePEIXOTO, R. M.LIMA, A. M. C.DAMASCENO, E. M.SOUZA, S. C. R.TEIXEIRA, M. F. da S.2020-11-30T09:01:36Z2020-11-30T09:01:36Z2020-11-292020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePLoSONE, v. 15, n. 11, e0239916, Nov. 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127175https://doi.org/10.1371/journal.pone.0239916enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-11-30T09:01:44Zoai:www.alice.cnptia.embrapa.br:doc/1127175Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-11-30T09:01:44falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-11-30T09:01:44Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Vertical transmissibility of small ruminant lentivirus. |
title |
Vertical transmissibility of small ruminant lentivirus. |
spellingShingle |
Vertical transmissibility of small ruminant lentivirus. ARAÚJO, J. F. SRLV Genetic techniques and protocols Lentivirus Sheep diseases Goat diseases Goats |
title_short |
Vertical transmissibility of small ruminant lentivirus. |
title_full |
Vertical transmissibility of small ruminant lentivirus. |
title_fullStr |
Vertical transmissibility of small ruminant lentivirus. |
title_full_unstemmed |
Vertical transmissibility of small ruminant lentivirus. |
title_sort |
Vertical transmissibility of small ruminant lentivirus. |
author |
ARAÚJO, J. F. |
author_facet |
ARAÚJO, J. F. ANDRIOLI, A. PINHEIRO, R. R. SIDER, L. H. SOUSA, A. L. M. de AZEVEDO, D. A. A. de PEIXOTO, R. M. LIMA, A. M. C. DAMASCENO, E. M. SOUZA, S. C. R. TEIXEIRA, M. F. da S. |
author_role |
author |
author2 |
ANDRIOLI, A. PINHEIRO, R. R. SIDER, L. H. SOUSA, A. L. M. de AZEVEDO, D. A. A. de PEIXOTO, R. M. LIMA, A. M. C. DAMASCENO, E. M. SOUZA, S. C. R. TEIXEIRA, M. F. da S. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
JUSCILÂNIA FURTADO ARAÚJO; ALICE ANDRIOLI, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; LUCIA HELENA SIDER, CNPC; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO; RENATO MESQUITA PEIXOTO; ANA MILENA CESAR LIMA; EDGAR MARQUES DAMASCENO; SAMARA CRISTINA ROCHA SOUZA; MARIA FÁTIMA DA SILVA TEIXEIRA. |
dc.contributor.author.fl_str_mv |
ARAÚJO, J. F. ANDRIOLI, A. PINHEIRO, R. R. SIDER, L. H. SOUSA, A. L. M. de AZEVEDO, D. A. A. de PEIXOTO, R. M. LIMA, A. M. C. DAMASCENO, E. M. SOUZA, S. C. R. TEIXEIRA, M. F. da S. |
dc.subject.por.fl_str_mv |
SRLV Genetic techniques and protocols Lentivirus Sheep diseases Goat diseases Goats |
topic |
SRLV Genetic techniques and protocols Lentivirus Sheep diseases Goat diseases Goats |
description |
Abstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-30T09:01:36Z 2020-11-30T09:01:36Z 2020-11-29 2020 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PLoSONE, v. 15, n. 11, e0239916, Nov. 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127175 https://doi.org/10.1371/journal.pone.0239916 |
identifier_str_mv |
PLoSONE, v. 15, n. 11, e0239916, Nov. 2020. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127175 https://doi.org/10.1371/journal.pone.0239916 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503498611556352 |