Agrobacterium-mediated genetic transformation of a tropical elite maize line.
Main Author: | |
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Publication Date: | 2017 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Download full: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076138 |
Summary: | ABSTRACT: The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% ? half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A. tumefaciens harboring the binary vector pTF102 containing the genes uidA and BAR under control of the CaMV35S promoter. The efficiency of the transgenic events produced was 3% for full strength and 1.1% for half strength N6 salts. Although under a lower concentration of salts, GUS expression was stronger; under this concentration, regeneration was less efficient. Thus, the results showed that the presence of 100% N6 salts in the infection and cocultivation media favored genetic transformation of the L3 maize inbred line mediated by A. tumefaciens. |
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Agrobacterium-mediated genetic transformation of a tropical elite maize line.Concentração de salTransformação genéticaEmbriogêneseGenéticaBactériaABSTRACT: The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% ? half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A. tumefaciens harboring the binary vector pTF102 containing the genes uidA and BAR under control of the CaMV35S promoter. The efficiency of the transgenic events produced was 3% for full strength and 1.1% for half strength N6 salts. Although under a lower concentration of salts, GUS expression was stronger; under this concentration, regeneration was less efficient. Thus, the results showed that the presence of 100% N6 salts in the infection and cocultivation media favored genetic transformation of the L3 maize inbred line mediated by A. tumefaciens.Rafaeli Aparecida Vieira de Souza, Universidade Federal de Viçosa; MEIRE DE CASSIA ALVES, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS; BEATRIZ DE ALMEIDA BARROS, CNPMS; Aluízio Borém, Universidade Federal de Viçosa; ANDREA ALMEIDA CARNEIRO, CNPMS.SOUZA, R. A. V. deALVES, M. de C.CARNEIRO, N. P.BARROS, B. de A.BOREM, A.CARNEIRO, A. A.2017-09-24T00:13:05Z2017-09-24T00:13:05Z2017-09-2220172018-01-30T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, p. 133-140, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/107613810.1590/1984-70332017v17n2a20enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-09-24T00:13:05Zoai:www.alice.cnptia.embrapa.br:doc/1076138Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-09-24T00:13:05falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-09-24T00:13:05Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Agrobacterium-mediated genetic transformation of a tropical elite maize line. |
title |
Agrobacterium-mediated genetic transformation of a tropical elite maize line. |
spellingShingle |
Agrobacterium-mediated genetic transformation of a tropical elite maize line. SOUZA, R. A. V. de Concentração de sal Transformação genética Embriogênese Genética Bactéria |
title_short |
Agrobacterium-mediated genetic transformation of a tropical elite maize line. |
title_full |
Agrobacterium-mediated genetic transformation of a tropical elite maize line. |
title_fullStr |
Agrobacterium-mediated genetic transformation of a tropical elite maize line. |
title_full_unstemmed |
Agrobacterium-mediated genetic transformation of a tropical elite maize line. |
title_sort |
Agrobacterium-mediated genetic transformation of a tropical elite maize line. |
author |
SOUZA, R. A. V. de |
author_facet |
SOUZA, R. A. V. de ALVES, M. de C. CARNEIRO, N. P. BARROS, B. de A. BOREM, A. CARNEIRO, A. A. |
author_role |
author |
author2 |
ALVES, M. de C. CARNEIRO, N. P. BARROS, B. de A. BOREM, A. CARNEIRO, A. A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Rafaeli Aparecida Vieira de Souza, Universidade Federal de Viçosa; MEIRE DE CASSIA ALVES, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS; BEATRIZ DE ALMEIDA BARROS, CNPMS; Aluízio Borém, Universidade Federal de Viçosa; ANDREA ALMEIDA CARNEIRO, CNPMS. |
dc.contributor.author.fl_str_mv |
SOUZA, R. A. V. de ALVES, M. de C. CARNEIRO, N. P. BARROS, B. de A. BOREM, A. CARNEIRO, A. A. |
dc.subject.por.fl_str_mv |
Concentração de sal Transformação genética Embriogênese Genética Bactéria |
topic |
Concentração de sal Transformação genética Embriogênese Genética Bactéria |
description |
ABSTRACT: The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% ? half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A. tumefaciens harboring the binary vector pTF102 containing the genes uidA and BAR under control of the CaMV35S promoter. The efficiency of the transgenic events produced was 3% for full strength and 1.1% for half strength N6 salts. Although under a lower concentration of salts, GUS expression was stronger; under this concentration, regeneration was less efficient. Thus, the results showed that the presence of 100% N6 salts in the infection and cocultivation media favored genetic transformation of the L3 maize inbred line mediated by A. tumefaciens. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-24T00:13:05Z 2017-09-24T00:13:05Z 2017-09-22 2017 2018-01-30T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, p. 133-140, 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076138 10.1590/1984-70332017v17n2a20 |
identifier_str_mv |
Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, p. 133-140, 2017. 10.1590/1984-70332017v17n2a20 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076138 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503442593480704 |