Assessment of the virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1158893 https://doi.org/10.3390/microorganisms11102597 |
Resumo: | ABSTRACT - Burkholderia mallei is an aerobic, Gram-negative, non-motile bacillus. As an obligate mammalian pathogen, it primarily affects solipeds. Although rarely transmitted to humans, the disease it causes, glanders, is classified as a zoonosis. The bacterium was officially eradicated in Brazil in 1969; however, it reemerged after three decades. This study aims to assess the virulence of a specific B. mallei strain, isolated in Brazil, in BALB/c mice through intranasal infection. The strain, B. mallei BAC 86/19, was obtained from the tracheal secretion of a young mare displaying positive serology but no clinical signs of glanders. Post-mortem examinations revealed macroscopic lesions consistent with the disease, however. In mice, the LD50 was determined to be approximately 1.59 × 105 colony-forming units (CFU)/animal. Mice exposed to either 0.1 × LD50 or 1 × LD50 displayed transient weight loss, which resolved after three or five days, respectively. B. mallei persisted within the liver and lung for five days post-infection and in the spleen for seven days. These findings underscore the detectable virulence of the Brazilian B. mallei BAC 86/19 strain in mice, which are relatively resilient hosts. This research points to the importance of the continued investigation of the virulence mechanisms and potential countermeasures associated with B. mallei infections, including their Brazilian isolates. |
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Assessment of the virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice.Doença AnimalZoonoseAnimal diseasesAnimal modelsBurkholderia malleiGlandersPathogenicityVirulenceZoonosesABSTRACT - Burkholderia mallei is an aerobic, Gram-negative, non-motile bacillus. As an obligate mammalian pathogen, it primarily affects solipeds. Although rarely transmitted to humans, the disease it causes, glanders, is classified as a zoonosis. The bacterium was officially eradicated in Brazil in 1969; however, it reemerged after three decades. This study aims to assess the virulence of a specific B. mallei strain, isolated in Brazil, in BALB/c mice through intranasal infection. The strain, B. mallei BAC 86/19, was obtained from the tracheal secretion of a young mare displaying positive serology but no clinical signs of glanders. Post-mortem examinations revealed macroscopic lesions consistent with the disease, however. In mice, the LD50 was determined to be approximately 1.59 × 105 colony-forming units (CFU)/animal. Mice exposed to either 0.1 × LD50 or 1 × LD50 displayed transient weight loss, which resolved after three or five days, respectively. B. mallei persisted within the liver and lung for five days post-infection and in the spleen for seven days. These findings underscore the detectable virulence of the Brazilian B. mallei BAC 86/19 strain in mice, which are relatively resilient hosts. This research points to the importance of the continued investigation of the virulence mechanisms and potential countermeasures associated with B. mallei infections, including their Brazilian isolates.EMANUELLE BALDO GASPAR, CPPSUL; LENITA RAMIRES DOS SANTOS, CNPGC; ANDREA ALVES DO EGITO, CNPGC; MARIA GORETTI DOS SANTOS, CNPGC; CYNTHIA MANTOVANI, CNPGC; JULIANA DA SILVA GOMES RIEGER, CNPGC; GUILHERME AUGUSTO DE SOUSA ABRANTES, CNPGC; PAULA ADAS PEREIRA SUNIGA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; JÚLIA DE MENDONÇA FAVACHO, CNPGC; INGRID BATISTA PINTO, CNPGC; ALESSANDRA FIGUEIREDO DE CASTRO NASSAR, CENTER FOR RESEARCH AND DEVELOPMENT IN ANIMAL HEALTH; FERNANDO LEANDRO DOS SANTOS, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; FLABIO RIBEIRO DE ARAUJO, CNPGC.GASPAR, E. B.SANTOS, L. R. dosEGITO, A. A. doSANTOS, M. G. dosMANTOVANI, C.RIEGER, J. DA S. G.ABRANTES, G. A. DE S.SUNIGA, P. A. P.FAVACHO, J. DE M.PINTO, I. B.NASSAR, A. F. DE C.SANTOS, F. L. DOSARAUJO, F. R.2023-11-29T19:37:05Z2023-11-29T19:37:05Z2023-11-292023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMicroorganisms, v. 11, issue 10, e2597, 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1158893https://doi.org/10.3390/microorganisms11102597enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-11-29T19:37:05Zoai:www.alice.cnptia.embrapa.br:doc/1158893Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-11-29T19:37:05falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-11-29T19:37:05Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Assessment of the virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice. |
title |
Assessment of the virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice. |
spellingShingle |
Assessment of the virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice. GASPAR, E. B. Doença Animal Zoonose Animal diseases Animal models Burkholderia mallei Glanders Pathogenicity Virulence Zoonoses |
title_short |
Assessment of the virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice. |
title_full |
Assessment of the virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice. |
title_fullStr |
Assessment of the virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice. |
title_full_unstemmed |
Assessment of the virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice. |
title_sort |
Assessment of the virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice. |
author |
GASPAR, E. B. |
author_facet |
GASPAR, E. B. SANTOS, L. R. dos EGITO, A. A. do SANTOS, M. G. dos MANTOVANI, C. RIEGER, J. DA S. G. ABRANTES, G. A. DE S. SUNIGA, P. A. P. FAVACHO, J. DE M. PINTO, I. B. NASSAR, A. F. DE C. SANTOS, F. L. DOS ARAUJO, F. R. |
author_role |
author |
author2 |
SANTOS, L. R. dos EGITO, A. A. do SANTOS, M. G. dos MANTOVANI, C. RIEGER, J. DA S. G. ABRANTES, G. A. DE S. SUNIGA, P. A. P. FAVACHO, J. DE M. PINTO, I. B. NASSAR, A. F. DE C. SANTOS, F. L. DOS ARAUJO, F. R. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
EMANUELLE BALDO GASPAR, CPPSUL; LENITA RAMIRES DOS SANTOS, CNPGC; ANDREA ALVES DO EGITO, CNPGC; MARIA GORETTI DOS SANTOS, CNPGC; CYNTHIA MANTOVANI, CNPGC; JULIANA DA SILVA GOMES RIEGER, CNPGC; GUILHERME AUGUSTO DE SOUSA ABRANTES, CNPGC; PAULA ADAS PEREIRA SUNIGA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; JÚLIA DE MENDONÇA FAVACHO, CNPGC; INGRID BATISTA PINTO, CNPGC; ALESSANDRA FIGUEIREDO DE CASTRO NASSAR, CENTER FOR RESEARCH AND DEVELOPMENT IN ANIMAL HEALTH; FERNANDO LEANDRO DOS SANTOS, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; FLABIO RIBEIRO DE ARAUJO, CNPGC. |
dc.contributor.author.fl_str_mv |
GASPAR, E. B. SANTOS, L. R. dos EGITO, A. A. do SANTOS, M. G. dos MANTOVANI, C. RIEGER, J. DA S. G. ABRANTES, G. A. DE S. SUNIGA, P. A. P. FAVACHO, J. DE M. PINTO, I. B. NASSAR, A. F. DE C. SANTOS, F. L. DOS ARAUJO, F. R. |
dc.subject.por.fl_str_mv |
Doença Animal Zoonose Animal diseases Animal models Burkholderia mallei Glanders Pathogenicity Virulence Zoonoses |
topic |
Doença Animal Zoonose Animal diseases Animal models Burkholderia mallei Glanders Pathogenicity Virulence Zoonoses |
description |
ABSTRACT - Burkholderia mallei is an aerobic, Gram-negative, non-motile bacillus. As an obligate mammalian pathogen, it primarily affects solipeds. Although rarely transmitted to humans, the disease it causes, glanders, is classified as a zoonosis. The bacterium was officially eradicated in Brazil in 1969; however, it reemerged after three decades. This study aims to assess the virulence of a specific B. mallei strain, isolated in Brazil, in BALB/c mice through intranasal infection. The strain, B. mallei BAC 86/19, was obtained from the tracheal secretion of a young mare displaying positive serology but no clinical signs of glanders. Post-mortem examinations revealed macroscopic lesions consistent with the disease, however. In mice, the LD50 was determined to be approximately 1.59 × 105 colony-forming units (CFU)/animal. Mice exposed to either 0.1 × LD50 or 1 × LD50 displayed transient weight loss, which resolved after three or five days, respectively. B. mallei persisted within the liver and lung for five days post-infection and in the spleen for seven days. These findings underscore the detectable virulence of the Brazilian B. mallei BAC 86/19 strain in mice, which are relatively resilient hosts. This research points to the importance of the continued investigation of the virulence mechanisms and potential countermeasures associated with B. mallei infections, including their Brazilian isolates. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-29T19:37:05Z 2023-11-29T19:37:05Z 2023-11-29 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Microorganisms, v. 11, issue 10, e2597, 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1158893 https://doi.org/10.3390/microorganisms11102597 |
identifier_str_mv |
Microorganisms, v. 11, issue 10, e2597, 2023. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1158893 https://doi.org/10.3390/microorganisms11102597 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503552591200256 |