Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/928502 https://doi.org/10.1016/j.cimid.2011.08.003 |
Resumo: | Leptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2?7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 107M−1 to 4 × 108M−1. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis. |
id |
EMBR_33cb2c3b4ed1da7c54088e0ac74821fb |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/928502 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions.MonoclonalLeptospiroseAnticorpo MonoclonalLeptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2?7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 107M−1 to 4 × 108M−1. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis.LEONARDO G. MONTE, UFPel; FABRICIO, R. CONCEIÇÃO, UFPel; MARIANA L. COUTINHO, UFPel; FABIANA K. SEIXAS, UFPel; EVERTON F. da SILVA, UFPel; FLÁVIA A. VASCONCELLOS, UFPel; LUIS ANTONIO SUITA DE CASTRO, CPACT; CLÁUDIA P. HARTLEBEN, UFPel; ODIR A. DELLAGOSTIN, UFPel; JOSÉ A. G. ALEIXO, UFPel.MONTE, L. G.CONCEIÇÃO, F. R.COUTINHO, M. L.SEIXAS, F. K.SILVA, E. V. daVASCONCELLOS, F. A.CASTRO, L. A. S. deHARTLEBEN, C. P.DELLAGOSTIN, O. A.ALEIXO, J. A. G.2020-05-18T04:37:49Z2020-05-18T04:37:49Z2012-07-172011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleComparative Immunology, Microbiology and Infectious Diseases, v. 34, n. 5, p. 441-446, set. 2011.0147-9571http://www.alice.cnptia.embrapa.br/alice/handle/doc/928502https://doi.org/10.1016/j.cimid.2011.08.003enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-05-18T04:38:01Zoai:www.alice.cnptia.embrapa.br:doc/928502Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-05-18T04:38:01falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-05-18T04:38:01Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. |
title |
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. |
spellingShingle |
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. MONTE, L. G. Monoclonal Leptospirose Anticorpo Monoclonal |
title_short |
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. |
title_full |
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. |
title_fullStr |
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. |
title_full_unstemmed |
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. |
title_sort |
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. |
author |
MONTE, L. G. |
author_facet |
MONTE, L. G. CONCEIÇÃO, F. R. COUTINHO, M. L. SEIXAS, F. K. SILVA, E. V. da VASCONCELLOS, F. A. CASTRO, L. A. S. de HARTLEBEN, C. P. DELLAGOSTIN, O. A. ALEIXO, J. A. G. |
author_role |
author |
author2 |
CONCEIÇÃO, F. R. COUTINHO, M. L. SEIXAS, F. K. SILVA, E. V. da VASCONCELLOS, F. A. CASTRO, L. A. S. de HARTLEBEN, C. P. DELLAGOSTIN, O. A. ALEIXO, J. A. G. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
LEONARDO G. MONTE, UFPel; FABRICIO, R. CONCEIÇÃO, UFPel; MARIANA L. COUTINHO, UFPel; FABIANA K. SEIXAS, UFPel; EVERTON F. da SILVA, UFPel; FLÁVIA A. VASCONCELLOS, UFPel; LUIS ANTONIO SUITA DE CASTRO, CPACT; CLÁUDIA P. HARTLEBEN, UFPel; ODIR A. DELLAGOSTIN, UFPel; JOSÉ A. G. ALEIXO, UFPel. |
dc.contributor.author.fl_str_mv |
MONTE, L. G. CONCEIÇÃO, F. R. COUTINHO, M. L. SEIXAS, F. K. SILVA, E. V. da VASCONCELLOS, F. A. CASTRO, L. A. S. de HARTLEBEN, C. P. DELLAGOSTIN, O. A. ALEIXO, J. A. G. |
dc.subject.por.fl_str_mv |
Monoclonal Leptospirose Anticorpo Monoclonal |
topic |
Monoclonal Leptospirose Anticorpo Monoclonal |
description |
Leptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2?7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 107M−1 to 4 × 108M−1. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2012-07-17 2020-05-18T04:37:49Z 2020-05-18T04:37:49Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Comparative Immunology, Microbiology and Infectious Diseases, v. 34, n. 5, p. 441-446, set. 2011. 0147-9571 http://www.alice.cnptia.embrapa.br/alice/handle/doc/928502 https://doi.org/10.1016/j.cimid.2011.08.003 |
identifier_str_mv |
Comparative Immunology, Microbiology and Infectious Diseases, v. 34, n. 5, p. 441-446, set. 2011. 0147-9571 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/928502 https://doi.org/10.1016/j.cimid.2011.08.003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503492278157312 |