Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1002812 |
Resumo: | Cytokines are small cell-signaling proteins that play an important role in the immune system, participating in intracellular communication. Four candidate genes of the cytokine family (IL2, IL4, IL13, and IFNG) were selected to identify Single Nucleotide Polymorphisms (SNPs) that might be associated with resistance to gastrointestinal endoparasites in goats. A population of 229 goats, F2 offspring from an F1 intercross was produced by crossing pure Saanen goats, considered as susceptible to gastrointestinal endoparasites, with pure Anglo-Nubian goats, considered resistant. Blood was collected for DNA extraction and fecal samples were also collected for parasite egg count. Polymorphisms were prospected by sequencing animals with extreme phenotype for fecal egg count (FEC) distribution. The association between SNPs and phenotype was determined by using the Fisher exact test with correction for multiple tests. Three of the 10 SNPs were identified as significant (P ≤ 0.03). They were found in intron 1 of IL2 (ENSBTA00000020883), intron 3 of IL13 (ENSBTA00000015953) and exon 3 of IFNG (ENSBTA00000012529), suggesting an association between them and gastrointestinal endoparasite resistance. Further studies will help describe the effects of these markers accurately before implementing them in marker assisted selection. This study is the pioneer in describing such associations in goats. |
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Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats.CytokineGastrointestinal infectionCapra Hircussingle nucleotide polymorphismCytokines are small cell-signaling proteins that play an important role in the immune system, participating in intracellular communication. Four candidate genes of the cytokine family (IL2, IL4, IL13, and IFNG) were selected to identify Single Nucleotide Polymorphisms (SNPs) that might be associated with resistance to gastrointestinal endoparasites in goats. A population of 229 goats, F2 offspring from an F1 intercross was produced by crossing pure Saanen goats, considered as susceptible to gastrointestinal endoparasites, with pure Anglo-Nubian goats, considered resistant. Blood was collected for DNA extraction and fecal samples were also collected for parasite egg count. Polymorphisms were prospected by sequencing animals with extreme phenotype for fecal egg count (FEC) distribution. The association between SNPs and phenotype was determined by using the Fisher exact test with correction for multiple tests. Three of the 10 SNPs were identified as significant (P ≤ 0.03). They were found in intron 1 of IL2 (ENSBTA00000020883), intron 3 of IL13 (ENSBTA00000015953) and exon 3 of IFNG (ENSBTA00000012529), suggesting an association between them and gastrointestinal endoparasite resistance. Further studies will help describe the effects of these markers accurately before implementing them in marker assisted selection. This study is the pioneer in describing such associations in goats.FLAVIA ALINE BRESSANI, CPPSEP. C. TIZIOTO, Universidade Federal de São CarlosRODRIGO GIGLIOTI, Universidade Estadual PaulistaS. L. C. MEIRELLES, Universidade Federal de LavrasR. COUTINHO, Universidade Federal do Rio Grande do NorteC. L. BENVENUTI, Universidade Federal do Rio Grande do NorteWILSON MALAGO JUNIOR, CPPSEMAURICIO DE ALVARENGA MUDADU, CPPSELUIS CARLOS VIEIRA, CNPCL. G. ZAROS, Universidade Federal do Rio Grande do NorteE. CARRILHO, Universidade de São PauloLUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.BRESSANI, F. A.TIZIOTO, P. C.GIGLIOTI, R.MEIRELLHES, S. L. C.COUTINHO, R.BENVENUTI, C. L.MALAGO JUNIOR, W.MUDADU, M. de A.VIEIRA, L. C.ZAROS, L. G.CARRILHO, E.REGITANO, L. C. de A.2014-12-16T11:11:11Z2014-12-16T11:11:11Z2014-12-1620142016-03-01T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 13, n. 4, p. 8530-8436, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/100281210.4238/2014.October.20.29enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:59:27Zoai:www.alice.cnptia.embrapa.br:doc/1002812Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:59:27falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:59:27Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats. |
title |
Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats. |
spellingShingle |
Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats. BRESSANI, F. A. Cytokine Gastrointestinal infection Capra Hircus single nucleotide polymorphism |
title_short |
Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats. |
title_full |
Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats. |
title_fullStr |
Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats. |
title_full_unstemmed |
Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats. |
title_sort |
Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats. |
author |
BRESSANI, F. A. |
author_facet |
BRESSANI, F. A. TIZIOTO, P. C. GIGLIOTI, R. MEIRELLHES, S. L. C. COUTINHO, R. BENVENUTI, C. L. MALAGO JUNIOR, W. MUDADU, M. de A. VIEIRA, L. C. ZAROS, L. G. CARRILHO, E. REGITANO, L. C. de A. |
author_role |
author |
author2 |
TIZIOTO, P. C. GIGLIOTI, R. MEIRELLHES, S. L. C. COUTINHO, R. BENVENUTI, C. L. MALAGO JUNIOR, W. MUDADU, M. de A. VIEIRA, L. C. ZAROS, L. G. CARRILHO, E. REGITANO, L. C. de A. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
FLAVIA ALINE BRESSANI, CPPSE P. C. TIZIOTO, Universidade Federal de São Carlos RODRIGO GIGLIOTI, Universidade Estadual Paulista S. L. C. MEIRELLES, Universidade Federal de Lavras R. COUTINHO, Universidade Federal do Rio Grande do Norte C. L. BENVENUTI, Universidade Federal do Rio Grande do Norte WILSON MALAGO JUNIOR, CPPSE MAURICIO DE ALVARENGA MUDADU, CPPSE LUIS CARLOS VIEIRA, CNPC L. G. ZAROS, Universidade Federal do Rio Grande do Norte E. CARRILHO, Universidade de São Paulo LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
dc.contributor.author.fl_str_mv |
BRESSANI, F. A. TIZIOTO, P. C. GIGLIOTI, R. MEIRELLHES, S. L. C. COUTINHO, R. BENVENUTI, C. L. MALAGO JUNIOR, W. MUDADU, M. de A. VIEIRA, L. C. ZAROS, L. G. CARRILHO, E. REGITANO, L. C. de A. |
dc.subject.por.fl_str_mv |
Cytokine Gastrointestinal infection Capra Hircus single nucleotide polymorphism |
topic |
Cytokine Gastrointestinal infection Capra Hircus single nucleotide polymorphism |
description |
Cytokines are small cell-signaling proteins that play an important role in the immune system, participating in intracellular communication. Four candidate genes of the cytokine family (IL2, IL4, IL13, and IFNG) were selected to identify Single Nucleotide Polymorphisms (SNPs) that might be associated with resistance to gastrointestinal endoparasites in goats. A population of 229 goats, F2 offspring from an F1 intercross was produced by crossing pure Saanen goats, considered as susceptible to gastrointestinal endoparasites, with pure Anglo-Nubian goats, considered resistant. Blood was collected for DNA extraction and fecal samples were also collected for parasite egg count. Polymorphisms were prospected by sequencing animals with extreme phenotype for fecal egg count (FEC) distribution. The association between SNPs and phenotype was determined by using the Fisher exact test with correction for multiple tests. Three of the 10 SNPs were identified as significant (P ≤ 0.03). They were found in intron 1 of IL2 (ENSBTA00000020883), intron 3 of IL13 (ENSBTA00000015953) and exon 3 of IFNG (ENSBTA00000012529), suggesting an association between them and gastrointestinal endoparasite resistance. Further studies will help describe the effects of these markers accurately before implementing them in marker assisted selection. This study is the pioneer in describing such associations in goats. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-16T11:11:11Z 2014-12-16T11:11:11Z 2014-12-16 2014 2016-03-01T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, v. 13, n. 4, p. 8530-8436, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1002812 10.4238/2014.October.20.29 |
identifier_str_mv |
Genetics and Molecular Research, v. 13, n. 4, p. 8530-8436, 2014. 10.4238/2014.October.20.29 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1002812 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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