Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149297 https://doi.org/ 10.1371/journal.pone.0273959 |
Resumo: | Tropical fruit tree species constitute a yet untapped supply of outstanding diversity of taste and nutritional value, barely developed from the genetics standpoint, with scarce or no genomic resources to tackle the challenges arising in modern breeding practice. We generated a de novo genome assembly of the Psidium guajava, the super fruit “apple of the tropics”, and successfully transferred 14,268 SNP probesets from Eucalyptus to Psidium at the nucleo- tide level, to detect genomic loci linked to resistance to the root knot nematode (RKN) Meloi- dogyne enterolobii derived from the wild relative P. guineense. Significantly associated loci with resistance across alternative analytical frameworks, were detected at two SNPs on chromosome 3 in a pseudo-assembly of Psidium guajava genome built using a syntenic path approach with the Eucalyptus grandis genome to determine the order and orientation of the contigs. The P. guineense-derived resistance response to RKN and disease onset is conceivably triggered by mineral nutrients and phytohormone homeostasis or signaling with the involvement of the miRNA pathway. Hotspots of mapped resistance quantitative trait loci and functional annotation in the same genomic region of Eucalyptus provide further indirect support to our results, highlighting the evolutionary conservation of genomes across genera of Myrtaceae in the adaptation to pathogens. Marker assisted introgression of the resistance loci mapped should accelerate the development of improved guava cultivars and hybrid rootstocks. |
id |
EMBR_356d350d08df074ae643bc8853cb4b8c |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1149297 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae.Meloidogyne enterolobiiMyrtaceaeGoiabaMelhoramento Genético VegetalPsidium GuajavaGuavasPsidiumTropical fruit tree species constitute a yet untapped supply of outstanding diversity of taste and nutritional value, barely developed from the genetics standpoint, with scarce or no genomic resources to tackle the challenges arising in modern breeding practice. We generated a de novo genome assembly of the Psidium guajava, the super fruit “apple of the tropics”, and successfully transferred 14,268 SNP probesets from Eucalyptus to Psidium at the nucleo- tide level, to detect genomic loci linked to resistance to the root knot nematode (RKN) Meloi- dogyne enterolobii derived from the wild relative P. guineense. Significantly associated loci with resistance across alternative analytical frameworks, were detected at two SNPs on chromosome 3 in a pseudo-assembly of Psidium guajava genome built using a syntenic path approach with the Eucalyptus grandis genome to determine the order and orientation of the contigs. The P. guineense-derived resistance response to RKN and disease onset is conceivably triggered by mineral nutrients and phytohormone homeostasis or signaling with the involvement of the miRNA pathway. Hotspots of mapped resistance quantitative trait loci and functional annotation in the same genomic region of Eucalyptus provide further indirect support to our results, highlighting the evolutionary conservation of genomes across genera of Myrtaceae in the adaptation to pathogens. Marker assisted introgression of the resistance loci mapped should accelerate the development of improved guava cultivars and hybrid rootstocks.CARLOS ANTONIO FERNANDES SANTOS, CPATSA; SONIANE RODRIGUES DA COSTA, Universidade Estadual de Feira de Santana, Feira de Santana, Bahia; LEONARDO SILVA BOITEUX, CNPH; DARIO GRATTAPAGLIA, Cenargen; ORZENIL BONFIM DA SILVA JUNIOR, Cenargen.SANTOS, C. A. F.COSTA, S. R. daBOITEUX, L. S.GRATTAPAGLIA, D.SILVA JUNIOR, O. B. da2022-12-07T13:01:25Z2022-12-07T13:01:25Z2022-12-072022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePLoS ONE, v. 17, n. 11, e0273959, 2022.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149297https://doi.org/ 10.1371/journal.pone.0273959enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-12-07T13:01:25Zoai:www.alice.cnptia.embrapa.br:doc/1149297Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-12-07T13:01:25falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-12-07T13:01:25Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae. |
title |
Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae. |
spellingShingle |
Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae. SANTOS, C. A. F. Meloidogyne enterolobii Myrtaceae Goiaba Melhoramento Genético Vegetal Psidium Guajava Guavas Psidium |
title_short |
Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae. |
title_full |
Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae. |
title_fullStr |
Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae. |
title_full_unstemmed |
Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae. |
title_sort |
Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae. |
author |
SANTOS, C. A. F. |
author_facet |
SANTOS, C. A. F. COSTA, S. R. da BOITEUX, L. S. GRATTAPAGLIA, D. SILVA JUNIOR, O. B. da |
author_role |
author |
author2 |
COSTA, S. R. da BOITEUX, L. S. GRATTAPAGLIA, D. SILVA JUNIOR, O. B. da |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
CARLOS ANTONIO FERNANDES SANTOS, CPATSA; SONIANE RODRIGUES DA COSTA, Universidade Estadual de Feira de Santana, Feira de Santana, Bahia; LEONARDO SILVA BOITEUX, CNPH; DARIO GRATTAPAGLIA, Cenargen; ORZENIL BONFIM DA SILVA JUNIOR, Cenargen. |
dc.contributor.author.fl_str_mv |
SANTOS, C. A. F. COSTA, S. R. da BOITEUX, L. S. GRATTAPAGLIA, D. SILVA JUNIOR, O. B. da |
dc.subject.por.fl_str_mv |
Meloidogyne enterolobii Myrtaceae Goiaba Melhoramento Genético Vegetal Psidium Guajava Guavas Psidium |
topic |
Meloidogyne enterolobii Myrtaceae Goiaba Melhoramento Genético Vegetal Psidium Guajava Guavas Psidium |
description |
Tropical fruit tree species constitute a yet untapped supply of outstanding diversity of taste and nutritional value, barely developed from the genetics standpoint, with scarce or no genomic resources to tackle the challenges arising in modern breeding practice. We generated a de novo genome assembly of the Psidium guajava, the super fruit “apple of the tropics”, and successfully transferred 14,268 SNP probesets from Eucalyptus to Psidium at the nucleo- tide level, to detect genomic loci linked to resistance to the root knot nematode (RKN) Meloi- dogyne enterolobii derived from the wild relative P. guineense. Significantly associated loci with resistance across alternative analytical frameworks, were detected at two SNPs on chromosome 3 in a pseudo-assembly of Psidium guajava genome built using a syntenic path approach with the Eucalyptus grandis genome to determine the order and orientation of the contigs. The P. guineense-derived resistance response to RKN and disease onset is conceivably triggered by mineral nutrients and phytohormone homeostasis or signaling with the involvement of the miRNA pathway. Hotspots of mapped resistance quantitative trait loci and functional annotation in the same genomic region of Eucalyptus provide further indirect support to our results, highlighting the evolutionary conservation of genomes across genera of Myrtaceae in the adaptation to pathogens. Marker assisted introgression of the resistance loci mapped should accelerate the development of improved guava cultivars and hybrid rootstocks. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-07T13:01:25Z 2022-12-07T13:01:25Z 2022-12-07 2022 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PLoS ONE, v. 17, n. 11, e0273959, 2022. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149297 https://doi.org/ 10.1371/journal.pone.0273959 |
identifier_str_mv |
PLoS ONE, v. 17, n. 11, e0273959, 2022. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149297 https://doi.org/ 10.1371/journal.pone.0273959 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503535608463360 |