In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/916487 |
Resumo: | Fusarium vascular wilt (caused by Fusarium oxysporum f. sp. passiflorae) is a limiting factor in the cultivation of yellow passion fruit (Passiflora edulis). Since there is no effective and economically viable control available, development of resistant or at least tolerant cultivars are in demand. A number of procedures have been used for the initial selection of plant genotypes resistant to various fungal pathogens by means of a fungal culture filtrate or purified toxin. In this study, seeds and in vitro-grown plantlets of passion fruit were screened with different concentrations of either Fusarium oxysporum f. sp. passiflorae (FOP) culture filtrate (0, 20, 30, 40 or 50%, v/v) or fusaric acid (0.10, 0.20, 0.30 or 0.40 mM) supplemented in Murashige and Skoog (MS) basal media. Subsequently, selected plants were inoculated with a conidial suspension of FOP to assess correlation between in vivo and in vitro responses. In vitro sensitivity to the selective agents and the resistance response to the pathogen were also compared. Root growth was markedly influenced by FA, culture filtrate, and conidial suspension culture treatments. Observations indicated that roots were primary targets for attack by F. oxysporum. Successful in vitro selection of resistant genotypes by both FA and culture filtrate treatments suggested that this strategy was viable for accelerating breeding of passion fruit for resistance to the Fusarium vascular wilt. |
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In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt.Maracujá amareloYellow passion fruitMaracuyá amarilloFusarium oxysporum f sp passifloraeFusariosisEnfermedades y desórdenes de las plantasCultivo in vitroPassiflora edulis f flavicarpaDoença de plantaMaracujá PerobaPassiflora edulis flavicarpaFungoFusarioseSeleção genótipaCultura in vitroVariedade resistenteCaracterísticas AgronômicasGenótipoPlant diseases and disordersFungiGenotypeAgronomic traitsIn vitro cultureFusarium vascular wilt (caused by Fusarium oxysporum f. sp. passiflorae) is a limiting factor in the cultivation of yellow passion fruit (Passiflora edulis). Since there is no effective and economically viable control available, development of resistant or at least tolerant cultivars are in demand. A number of procedures have been used for the initial selection of plant genotypes resistant to various fungal pathogens by means of a fungal culture filtrate or purified toxin. In this study, seeds and in vitro-grown plantlets of passion fruit were screened with different concentrations of either Fusarium oxysporum f. sp. passiflorae (FOP) culture filtrate (0, 20, 30, 40 or 50%, v/v) or fusaric acid (0.10, 0.20, 0.30 or 0.40 mM) supplemented in Murashige and Skoog (MS) basal media. Subsequently, selected plants were inoculated with a conidial suspension of FOP to assess correlation between in vivo and in vitro responses. In vitro sensitivity to the selective agents and the resistance response to the pathogen were also compared. Root growth was markedly influenced by FA, culture filtrate, and conidial suspension culture treatments. Observations indicated that roots were primary targets for attack by F. oxysporum. Successful in vitro selection of resistant genotypes by both FA and culture filtrate treatments suggested that this strategy was viable for accelerating breeding of passion fruit for resistance to the Fusarium vascular wilt.PATRICIA SILVA FLORES, CPAF-AC; WAGNER CAMPOS OTONI, UFV; ONKAR DEV DHINGRA, UFV; SÉRGIO PAULO SEVERO DE SOUZA DINIZ, UEM; TELMA MIRANDA DOS SANTOS; CLAUDIO HORST BRUCKNER.FLORES, P. S.OTONI, W. C.DHINGRA, O. D.DINIZ, S. P. S. de S.SANTOS, T. M. dosBRUCKNER, C. H.2012-02-24T11:11:11Z2012-02-24T11:11:11Z2012-02-2420122019-01-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlant Cell, Tissue and Organ Culture, Heidelberg, v. 108, n. 1, p. 37-45, Jan. 2012.0167-6857 (impresso) / 1573-5044 (online)http://www.alice.cnptia.embrapa.br/alice/handle/doc/91648710.1007/s11240-011-0009-5enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:25:47Zoai:www.alice.cnptia.embrapa.br:doc/916487Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:25:47falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:25:47Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt. |
title |
In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt. |
spellingShingle |
In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt. FLORES, P. S. Maracujá amarelo Yellow passion fruit Maracuyá amarillo Fusarium oxysporum f sp passiflorae Fusariosis Enfermedades y desórdenes de las plantas Cultivo in vitro Passiflora edulis f flavicarpa Doença de planta Maracujá Peroba Passiflora edulis flavicarpa Fungo Fusariose Seleção genótipa Cultura in vitro Variedade resistente Características Agronômicas Genótipo Plant diseases and disorders Fungi Genotype Agronomic traits In vitro culture |
title_short |
In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt. |
title_full |
In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt. |
title_fullStr |
In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt. |
title_full_unstemmed |
In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt. |
title_sort |
In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt. |
author |
FLORES, P. S. |
author_facet |
FLORES, P. S. OTONI, W. C. DHINGRA, O. D. DINIZ, S. P. S. de S. SANTOS, T. M. dos BRUCKNER, C. H. |
author_role |
author |
author2 |
OTONI, W. C. DHINGRA, O. D. DINIZ, S. P. S. de S. SANTOS, T. M. dos BRUCKNER, C. H. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
PATRICIA SILVA FLORES, CPAF-AC; WAGNER CAMPOS OTONI, UFV; ONKAR DEV DHINGRA, UFV; SÉRGIO PAULO SEVERO DE SOUZA DINIZ, UEM; TELMA MIRANDA DOS SANTOS; CLAUDIO HORST BRUCKNER. |
dc.contributor.author.fl_str_mv |
FLORES, P. S. OTONI, W. C. DHINGRA, O. D. DINIZ, S. P. S. de S. SANTOS, T. M. dos BRUCKNER, C. H. |
dc.subject.por.fl_str_mv |
Maracujá amarelo Yellow passion fruit Maracuyá amarillo Fusarium oxysporum f sp passiflorae Fusariosis Enfermedades y desórdenes de las plantas Cultivo in vitro Passiflora edulis f flavicarpa Doença de planta Maracujá Peroba Passiflora edulis flavicarpa Fungo Fusariose Seleção genótipa Cultura in vitro Variedade resistente Características Agronômicas Genótipo Plant diseases and disorders Fungi Genotype Agronomic traits In vitro culture |
topic |
Maracujá amarelo Yellow passion fruit Maracuyá amarillo Fusarium oxysporum f sp passiflorae Fusariosis Enfermedades y desórdenes de las plantas Cultivo in vitro Passiflora edulis f flavicarpa Doença de planta Maracujá Peroba Passiflora edulis flavicarpa Fungo Fusariose Seleção genótipa Cultura in vitro Variedade resistente Características Agronômicas Genótipo Plant diseases and disorders Fungi Genotype Agronomic traits In vitro culture |
description |
Fusarium vascular wilt (caused by Fusarium oxysporum f. sp. passiflorae) is a limiting factor in the cultivation of yellow passion fruit (Passiflora edulis). Since there is no effective and economically viable control available, development of resistant or at least tolerant cultivars are in demand. A number of procedures have been used for the initial selection of plant genotypes resistant to various fungal pathogens by means of a fungal culture filtrate or purified toxin. In this study, seeds and in vitro-grown plantlets of passion fruit were screened with different concentrations of either Fusarium oxysporum f. sp. passiflorae (FOP) culture filtrate (0, 20, 30, 40 or 50%, v/v) or fusaric acid (0.10, 0.20, 0.30 or 0.40 mM) supplemented in Murashige and Skoog (MS) basal media. Subsequently, selected plants were inoculated with a conidial suspension of FOP to assess correlation between in vivo and in vitro responses. In vitro sensitivity to the selective agents and the resistance response to the pathogen were also compared. Root growth was markedly influenced by FA, culture filtrate, and conidial suspension culture treatments. Observations indicated that roots were primary targets for attack by F. oxysporum. Successful in vitro selection of resistant genotypes by both FA and culture filtrate treatments suggested that this strategy was viable for accelerating breeding of passion fruit for resistance to the Fusarium vascular wilt. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02-24T11:11:11Z 2012-02-24T11:11:11Z 2012-02-24 2012 2019-01-10T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plant Cell, Tissue and Organ Culture, Heidelberg, v. 108, n. 1, p. 37-45, Jan. 2012. 0167-6857 (impresso) / 1573-5044 (online) http://www.alice.cnptia.embrapa.br/alice/handle/doc/916487 10.1007/s11240-011-0009-5 |
identifier_str_mv |
Plant Cell, Tissue and Organ Culture, Heidelberg, v. 108, n. 1, p. 37-45, Jan. 2012. 0167-6857 (impresso) / 1573-5044 (online) 10.1007/s11240-011-0009-5 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/916487 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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