Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150273 |
Resumo: | Cassava root rot disease is caused by a complex of soil-borne pathogens and has high economic impacts because it directly affects the tuberous roots, which are the main commercial product. This study aimed to evaluate cassava genotypes for resistance to root rot disease in a field with a previous history of high disease incidence. It also aimed to identify possible genomic regions associated with field resistance based on genome-wide association studies. A total of 148 genotypes from Embrapa Mandioca and Fruticultura were evaluated over two years, including improved materials and curated germplasms. Analysis of phenotypic data was conducted, as well as a genomic association analysis, based on the general linear model, mixed linear model, and fixed and random model circulating probability unification. The observed high disease index (ω) was directly correlated with genotype survival, affecting plant height, shoot yield, and fresh root yield. The genotypes were grouped into five clusters, which were classified according to level of root rot resistance (i.e., extremely susceptible, susceptible, moderately susceptible, moderately resistant, and resistant). The 10 genotypes with the best performance in the field were selected as potential progenitors for the development of segregating progenies. Estimates of genomic kinship between these genotypes ranged from -0.183 to 0.671. The genotypes BGM-1171 and BGM-1190 showed the lowest degree of kinship with the other selected sources of resistance. The genotypes BGM-0209, BGM-0398, and BGM-0659 showed negative kinship values with most elite varieties, while BGM-0659 presented negative kinship with all landraces. A genome-wide association analysis detected five significant single nucleotide polymorphisms related to defense mechanisms against biotic and abiotic stresses, with putative association with fresh root yield in soil infested with root rot pathogens. These findings can be utilized to develop molecular selection for root rot resistance in cassava. |
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Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits.MandiocaPodridão RadicularDoença de PlantaRaizPatógenoCassavaRoot diseasesPathogensPlant pathogensCassava root rot disease is caused by a complex of soil-borne pathogens and has high economic impacts because it directly affects the tuberous roots, which are the main commercial product. This study aimed to evaluate cassava genotypes for resistance to root rot disease in a field with a previous history of high disease incidence. It also aimed to identify possible genomic regions associated with field resistance based on genome-wide association studies. A total of 148 genotypes from Embrapa Mandioca and Fruticultura were evaluated over two years, including improved materials and curated germplasms. Analysis of phenotypic data was conducted, as well as a genomic association analysis, based on the general linear model, mixed linear model, and fixed and random model circulating probability unification. The observed high disease index (ω) was directly correlated with genotype survival, affecting plant height, shoot yield, and fresh root yield. The genotypes were grouped into five clusters, which were classified according to level of root rot resistance (i.e., extremely susceptible, susceptible, moderately susceptible, moderately resistant, and resistant). The 10 genotypes with the best performance in the field were selected as potential progenitors for the development of segregating progenies. Estimates of genomic kinship between these genotypes ranged from -0.183 to 0.671. The genotypes BGM-1171 and BGM-1190 showed the lowest degree of kinship with the other selected sources of resistance. The genotypes BGM-0209, BGM-0398, and BGM-0659 showed negative kinship values with most elite varieties, while BGM-0659 presented negative kinship with all landraces. A genome-wide association analysis detected five significant single nucleotide polymorphisms related to defense mechanisms against biotic and abiotic stresses, with putative association with fresh root yield in soil infested with root rot pathogens. These findings can be utilized to develop molecular selection for root rot resistance in cassava.CAMILA SANTIAGO HOHENFELD; ADRIANA RODRIGUES PASSOS; HELIO WILSON LEMOS DE CARVALHO, CPATC; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; EDER JORGE DE OLIVEIRA, CNPMF.HOHENFELD, C. S.PASSOS, A. R.CARVALHO, H. W. L. deOLIVEIRA, S. A. S. deOLIVEIRA, E. J. de2023-04-12T16:25:30Z2023-04-12T16:25:30Z2022-12-222022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePLos One, v. 17, n. 6, e0270020, June, 2022.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150273| https://doi.org/10.1371/journal.pone.0270020enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-04-12T16:25:30Zoai:www.alice.cnptia.embrapa.br:doc/1150273Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-04-12T16:25:30falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-04-12T16:25:30Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits. |
title |
Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits. |
spellingShingle |
Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits. HOHENFELD, C. S. Mandioca Podridão Radicular Doença de Planta Raiz Patógeno Cassava Root diseases Pathogens Plant pathogens |
title_short |
Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits. |
title_full |
Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits. |
title_fullStr |
Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits. |
title_full_unstemmed |
Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits. |
title_sort |
Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits. |
author |
HOHENFELD, C. S. |
author_facet |
HOHENFELD, C. S. PASSOS, A. R. CARVALHO, H. W. L. de OLIVEIRA, S. A. S. de OLIVEIRA, E. J. de |
author_role |
author |
author2 |
PASSOS, A. R. CARVALHO, H. W. L. de OLIVEIRA, S. A. S. de OLIVEIRA, E. J. de |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
CAMILA SANTIAGO HOHENFELD; ADRIANA RODRIGUES PASSOS; HELIO WILSON LEMOS DE CARVALHO, CPATC; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; EDER JORGE DE OLIVEIRA, CNPMF. |
dc.contributor.author.fl_str_mv |
HOHENFELD, C. S. PASSOS, A. R. CARVALHO, H. W. L. de OLIVEIRA, S. A. S. de OLIVEIRA, E. J. de |
dc.subject.por.fl_str_mv |
Mandioca Podridão Radicular Doença de Planta Raiz Patógeno Cassava Root diseases Pathogens Plant pathogens |
topic |
Mandioca Podridão Radicular Doença de Planta Raiz Patógeno Cassava Root diseases Pathogens Plant pathogens |
description |
Cassava root rot disease is caused by a complex of soil-borne pathogens and has high economic impacts because it directly affects the tuberous roots, which are the main commercial product. This study aimed to evaluate cassava genotypes for resistance to root rot disease in a field with a previous history of high disease incidence. It also aimed to identify possible genomic regions associated with field resistance based on genome-wide association studies. A total of 148 genotypes from Embrapa Mandioca and Fruticultura were evaluated over two years, including improved materials and curated germplasms. Analysis of phenotypic data was conducted, as well as a genomic association analysis, based on the general linear model, mixed linear model, and fixed and random model circulating probability unification. The observed high disease index (ω) was directly correlated with genotype survival, affecting plant height, shoot yield, and fresh root yield. The genotypes were grouped into five clusters, which were classified according to level of root rot resistance (i.e., extremely susceptible, susceptible, moderately susceptible, moderately resistant, and resistant). The 10 genotypes with the best performance in the field were selected as potential progenitors for the development of segregating progenies. Estimates of genomic kinship between these genotypes ranged from -0.183 to 0.671. The genotypes BGM-1171 and BGM-1190 showed the lowest degree of kinship with the other selected sources of resistance. The genotypes BGM-0209, BGM-0398, and BGM-0659 showed negative kinship values with most elite varieties, while BGM-0659 presented negative kinship with all landraces. A genome-wide association analysis detected five significant single nucleotide polymorphisms related to defense mechanisms against biotic and abiotic stresses, with putative association with fresh root yield in soil infested with root rot pathogens. These findings can be utilized to develop molecular selection for root rot resistance in cassava. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-22 2022 2023-04-12T16:25:30Z 2023-04-12T16:25:30Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PLos One, v. 17, n. 6, e0270020, June, 2022. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150273 | https://doi.org/10.1371/journal.pone.0270020 |
identifier_str_mv |
PLos One, v. 17, n. 6, e0270020, June, 2022. | https://doi.org/10.1371/journal.pone.0270020 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150273 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503543193862144 |