Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115347 https://doi.org/10.3389/fmicb.2019.02252 |
Resumo: | Abstract: The taxonomically diverse rhizosphere microbiome contributes to plant nutrition, growth and health, including protection against soil-borne pathogens. We previously showed that breeding for Fusarium-resistance in common bean changed the rhizosphere microbiome composition and functioning. Here, we assessed the impact of Fusarium-resistance breeding in common bean on microbiome physiology. Combined with metatranscriptome data, community-level physiological profiling by Biolog EcoPlate analyses revealed that the rhizosphere microbiome of the Fusarium-resistant accession was distinctly different from that of the Fusarium-susceptible accession, with higher consumption of amino acids and amines, higher metabolism of xylanase and sialidase, and higher expression of genes associated with nitrogen, phosphorus and iron metabolism. The resistome analysis indicates higher expression of soxR, which is involved in protecting bacteria against oxidative stress induced by a pathogen invasion. These results further support our hypothesis that breeding for resistance has unintentionally shaped the assembly and activity of the rhizobacterial community toward a higher abundance of specific rhizosphere competent bacterial taxa that can provide complementary protection against fungal root infections. |
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Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome.Plant-microbe interactionsMetatranscriptomeBiolog EcoPlateNutrientmetabolismResistomeFeijãoResistênciaMicrobiologia do SoloPopulação MicrobianaSoil-plant interactionsBeansDisease resistanceMicrobiomeCarbohydrate metabolismAbstract: The taxonomically diverse rhizosphere microbiome contributes to plant nutrition, growth and health, including protection against soil-borne pathogens. We previously showed that breeding for Fusarium-resistance in common bean changed the rhizosphere microbiome composition and functioning. Here, we assessed the impact of Fusarium-resistance breeding in common bean on microbiome physiology. Combined with metatranscriptome data, community-level physiological profiling by Biolog EcoPlate analyses revealed that the rhizosphere microbiome of the Fusarium-resistant accession was distinctly different from that of the Fusarium-susceptible accession, with higher consumption of amino acids and amines, higher metabolism of xylanase and sialidase, and higher expression of genes associated with nitrogen, phosphorus and iron metabolism. The resistome analysis indicates higher expression of soxR, which is involved in protecting bacteria against oxidative stress induced by a pathogen invasion. These results further support our hypothesis that breeding for resistance has unintentionally shaped the assembly and activity of the rhizobacterial community toward a higher abundance of specific rhizosphere competent bacterial taxa that can provide complementary protection against fungal root infections.LUCAS WILLIAM MENDES, CENA-USP; MIRIAM GONCALVES DE CHAVES, CENA-USP; MARILEY DE CASSIA FONSECA, CENA-USP; RODRIGO MENDES, CNPMA; JOOS M RAAIJMAKERS, Netherlands Institute of Ecology; SIU MUI TSAI, CENA-USP.MENDES, L. W.CHAVES, M. G. deFONSECA, M. de C.MENDES, R.RAAIJMAKERS, J. M.TSAI, S. M.2019-11-28T00:36:28Z2019-11-28T00:36:28Z2019-11-2720192019-11-28T00:36:28Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFrontiers in Microbiology, v. 10, 2019. Article 2252.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115347https://doi.org/10.3389/fmicb.2019.02252enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-11-28T00:36:34Zoai:www.alice.cnptia.embrapa.br:doc/1115347Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-11-28T00:36:34falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-11-28T00:36:34Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome. |
title |
Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome. |
spellingShingle |
Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome. MENDES, L. W. Plant-microbe interactions Metatranscriptome Biolog EcoPlate Nutrientmetabolism Resistome Feijão Resistência Microbiologia do Solo População Microbiana Soil-plant interactions Beans Disease resistance Microbiome Carbohydrate metabolism |
title_short |
Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome. |
title_full |
Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome. |
title_fullStr |
Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome. |
title_full_unstemmed |
Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome. |
title_sort |
Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome. |
author |
MENDES, L. W. |
author_facet |
MENDES, L. W. CHAVES, M. G. de FONSECA, M. de C. MENDES, R. RAAIJMAKERS, J. M. TSAI, S. M. |
author_role |
author |
author2 |
CHAVES, M. G. de FONSECA, M. de C. MENDES, R. RAAIJMAKERS, J. M. TSAI, S. M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
LUCAS WILLIAM MENDES, CENA-USP; MIRIAM GONCALVES DE CHAVES, CENA-USP; MARILEY DE CASSIA FONSECA, CENA-USP; RODRIGO MENDES, CNPMA; JOOS M RAAIJMAKERS, Netherlands Institute of Ecology; SIU MUI TSAI, CENA-USP. |
dc.contributor.author.fl_str_mv |
MENDES, L. W. CHAVES, M. G. de FONSECA, M. de C. MENDES, R. RAAIJMAKERS, J. M. TSAI, S. M. |
dc.subject.por.fl_str_mv |
Plant-microbe interactions Metatranscriptome Biolog EcoPlate Nutrientmetabolism Resistome Feijão Resistência Microbiologia do Solo População Microbiana Soil-plant interactions Beans Disease resistance Microbiome Carbohydrate metabolism |
topic |
Plant-microbe interactions Metatranscriptome Biolog EcoPlate Nutrientmetabolism Resistome Feijão Resistência Microbiologia do Solo População Microbiana Soil-plant interactions Beans Disease resistance Microbiome Carbohydrate metabolism |
description |
Abstract: The taxonomically diverse rhizosphere microbiome contributes to plant nutrition, growth and health, including protection against soil-borne pathogens. We previously showed that breeding for Fusarium-resistance in common bean changed the rhizosphere microbiome composition and functioning. Here, we assessed the impact of Fusarium-resistance breeding in common bean on microbiome physiology. Combined with metatranscriptome data, community-level physiological profiling by Biolog EcoPlate analyses revealed that the rhizosphere microbiome of the Fusarium-resistant accession was distinctly different from that of the Fusarium-susceptible accession, with higher consumption of amino acids and amines, higher metabolism of xylanase and sialidase, and higher expression of genes associated with nitrogen, phosphorus and iron metabolism. The resistome analysis indicates higher expression of soxR, which is involved in protecting bacteria against oxidative stress induced by a pathogen invasion. These results further support our hypothesis that breeding for resistance has unintentionally shaped the assembly and activity of the rhizobacterial community toward a higher abundance of specific rhizosphere competent bacterial taxa that can provide complementary protection against fungal root infections. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-28T00:36:28Z 2019-11-28T00:36:28Z 2019-11-27 2019 2019-11-28T00:36:28Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Frontiers in Microbiology, v. 10, 2019. Article 2252. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115347 https://doi.org/10.3389/fmicb.2019.02252 |
identifier_str_mv |
Frontiers in Microbiology, v. 10, 2019. Article 2252. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115347 https://doi.org/10.3389/fmicb.2019.02252 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503484555395072 |