Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa).
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Publication Date: | 2015 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Download full: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035196 |
Summary: | Gene expression related to drought response in the leaf tissues of two Brazilian upland cultivars, the drought-tolerant Douradão and the drought-sensitive Primavera, was analyzed. RNA-seq identified 27,618 transcripts in the Douradão cultivar, with 24,090 (87.2%) homologous to the rice database, and 27,221 transcripts in the Primavera cultivar, with 23,663 (86.9%) homologous to the rice database. Gene-expression analysis between control and water-deficient treatments revealed 493 and 1154 differentially expressed genes in Douradão and Primavera cultivars, respectively. Genes exclusively expressed under drought were identified for Douradão, including two genes of particular interest coding for the protein peroxidase precursor, which is involved in three distinct metabolic pathways. Comparisons between the two drought-exposed cultivars revealed 2314 genes were differentially expressed (978 upregulated, 1336 downregulated in Douradão). Six genes distributed across 4 different transcription factor families (bHLH, MYB, NAC, and WRKY) were identified, all of which were upregulated in Douradão compared to Primavera during drought. Most of the genes identified in Douradão activate metabolic pathways responsible for production of secondary metabolites and genes coding for enzymatically active signaling receptors. Quantitative PCR validation showed that most gene expression was in agreement with computational prediction of these transcripts. The transcripts identified here will define molecular markers for identification of Cis-acting elements to search for allelic variants of these genes through analysis of polymorphic SNPs in GenBank accessions of upland rice, aiming to develop cultivars with the best combination of these alleles, resulting in materials with high yield potential in the event of drought during the reproductive phase. |
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Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa).ArrozGeneResistência a secaVariedadeGene expression related to drought response in the leaf tissues of two Brazilian upland cultivars, the drought-tolerant Douradão and the drought-sensitive Primavera, was analyzed. RNA-seq identified 27,618 transcripts in the Douradão cultivar, with 24,090 (87.2%) homologous to the rice database, and 27,221 transcripts in the Primavera cultivar, with 23,663 (86.9%) homologous to the rice database. Gene-expression analysis between control and water-deficient treatments revealed 493 and 1154 differentially expressed genes in Douradão and Primavera cultivars, respectively. Genes exclusively expressed under drought were identified for Douradão, including two genes of particular interest coding for the protein peroxidase precursor, which is involved in three distinct metabolic pathways. Comparisons between the two drought-exposed cultivars revealed 2314 genes were differentially expressed (978 upregulated, 1336 downregulated in Douradão). Six genes distributed across 4 different transcription factor families (bHLH, MYB, NAC, and WRKY) were identified, all of which were upregulated in Douradão compared to Primavera during drought. Most of the genes identified in Douradão activate metabolic pathways responsible for production of secondary metabolites and genes coding for enzymatically active signaling receptors. Quantitative PCR validation showed that most gene expression was in agreement with computational prediction of these transcripts. The transcripts identified here will define molecular markers for identification of Cis-acting elements to search for allelic variants of these genes through analysis of polymorphic SNPs in GenBank accessions of upland rice, aiming to develop cultivars with the best combination of these alleles, resulting in materials with high yield potential in the event of drought during the reproductive phase.NEWTON PORTILHO CARNEIRO, CNPMS.SILVEIRA, R. D.ABREU, F. R. M.MAMIDI, S.McCLEAN, P. E.VIANELLO, R. P.LANNA, A. C.CARNEIRO, N. P.BRONDANI, C.2016-01-26T11:11:11Z2016-01-26T11:11:11Z2016-01-2620152016-02-02T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, Ribeirão Preto, v. 14, n. 3, p. 8181-8200, 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/103519610.4238/2015.July.27.6enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:35:43Zoai:www.alice.cnptia.embrapa.br:doc/1035196Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:35:43falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:35:43Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa). |
title |
Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa). |
spellingShingle |
Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa). SILVEIRA, R. D. Arroz Gene Resistência a seca Variedade |
title_short |
Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa). |
title_full |
Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa). |
title_fullStr |
Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa). |
title_full_unstemmed |
Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa). |
title_sort |
Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa). |
author |
SILVEIRA, R. D. |
author_facet |
SILVEIRA, R. D. ABREU, F. R. M. MAMIDI, S. McCLEAN, P. E. VIANELLO, R. P. LANNA, A. C. CARNEIRO, N. P. BRONDANI, C. |
author_role |
author |
author2 |
ABREU, F. R. M. MAMIDI, S. McCLEAN, P. E. VIANELLO, R. P. LANNA, A. C. CARNEIRO, N. P. BRONDANI, C. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
NEWTON PORTILHO CARNEIRO, CNPMS. |
dc.contributor.author.fl_str_mv |
SILVEIRA, R. D. ABREU, F. R. M. MAMIDI, S. McCLEAN, P. E. VIANELLO, R. P. LANNA, A. C. CARNEIRO, N. P. BRONDANI, C. |
dc.subject.por.fl_str_mv |
Arroz Gene Resistência a seca Variedade |
topic |
Arroz Gene Resistência a seca Variedade |
description |
Gene expression related to drought response in the leaf tissues of two Brazilian upland cultivars, the drought-tolerant Douradão and the drought-sensitive Primavera, was analyzed. RNA-seq identified 27,618 transcripts in the Douradão cultivar, with 24,090 (87.2%) homologous to the rice database, and 27,221 transcripts in the Primavera cultivar, with 23,663 (86.9%) homologous to the rice database. Gene-expression analysis between control and water-deficient treatments revealed 493 and 1154 differentially expressed genes in Douradão and Primavera cultivars, respectively. Genes exclusively expressed under drought were identified for Douradão, including two genes of particular interest coding for the protein peroxidase precursor, which is involved in three distinct metabolic pathways. Comparisons between the two drought-exposed cultivars revealed 2314 genes were differentially expressed (978 upregulated, 1336 downregulated in Douradão). Six genes distributed across 4 different transcription factor families (bHLH, MYB, NAC, and WRKY) were identified, all of which were upregulated in Douradão compared to Primavera during drought. Most of the genes identified in Douradão activate metabolic pathways responsible for production of secondary metabolites and genes coding for enzymatically active signaling receptors. Quantitative PCR validation showed that most gene expression was in agreement with computational prediction of these transcripts. The transcripts identified here will define molecular markers for identification of Cis-acting elements to search for allelic variants of these genes through analysis of polymorphic SNPs in GenBank accessions of upland rice, aiming to develop cultivars with the best combination of these alleles, resulting in materials with high yield potential in the event of drought during the reproductive phase. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2016-01-26T11:11:11Z 2016-01-26T11:11:11Z 2016-01-26 2016-02-02T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 3, p. 8181-8200, 2015. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035196 10.4238/2015.July.27.6 |
identifier_str_mv |
Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 3, p. 8181-8200, 2015. 10.4238/2015.July.27.6 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035196 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503416539512832 |