In vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108906 |
Resumo: | Background: Haemonchosis affects sheep husbandry and its treatment is often compromised due to the development of anthelminthic resistance. Plant-derived bioactive compounds have been studied as alternative to control Haemonchus contortus. The objective of this study was to evaluate the effect of Senecio brasiliensis extracts on H. contortus egg hatching and infective larvae migration. Results: The aqueous extract from dried and fresh plant and alkaloid-enriched fraction of the previously dried leaves of S. brasiliensis inhibited H. contortus egg hatching. The main plant compound in alkaloid fraction was integerrimine, a pyrrolizidine alkaloid (PA). However, the aqueous extract from dried plant displayed higher efficacy when compared to their alkaloid enriched or non-polar fractions, meaning that, although PAs contributed to the ovicidal effect, other compounds in the plant can also contribute to their effect. Furthermore, the aqueous extract from dried plant also had higher efficacy than aqueous extract from fresh plant in larvae migration inhibition. Finally, extract from dried plant presented low in vitro cytotoxic effect. Conclusion: Taken together our results suggest a good anthelmintic effect of S. brasiliensis, especially when aqueous extract is prepared from dried plant. Further in vivo studies should be performed focused on forms of administration of this extract in rearing sheep. |
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In vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus.Maria MolePlanta TóxicaExtrato VegetalAnti-HelmínticoNematóideBackground: Haemonchosis affects sheep husbandry and its treatment is often compromised due to the development of anthelminthic resistance. Plant-derived bioactive compounds have been studied as alternative to control Haemonchus contortus. The objective of this study was to evaluate the effect of Senecio brasiliensis extracts on H. contortus egg hatching and infective larvae migration. Results: The aqueous extract from dried and fresh plant and alkaloid-enriched fraction of the previously dried leaves of S. brasiliensis inhibited H. contortus egg hatching. The main plant compound in alkaloid fraction was integerrimine, a pyrrolizidine alkaloid (PA). However, the aqueous extract from dried plant displayed higher efficacy when compared to their alkaloid enriched or non-polar fractions, meaning that, although PAs contributed to the ovicidal effect, other compounds in the plant can also contribute to their effect. Furthermore, the aqueous extract from dried plant also had higher efficacy than aqueous extract from fresh plant in larvae migration inhibition. Finally, extract from dried plant presented low in vitro cytotoxic effect. Conclusion: Taken together our results suggest a good anthelmintic effect of S. brasiliensis, especially when aqueous extract is prepared from dried plant. Further in vivo studies should be performed focused on forms of administration of this extract in rearing sheep.SUELEN MENDONÇA SOARES, UPF; ROBERT DOMINGUES, CPPSUL; EMANUELLE BALDO GASPAR, CPPSUL; PATRÍCIO AZEVEDO DOS SANTOS, URCAMP; KIRLEY MARQUES CANUTO, CNPAT; ALESSANDRO PELEGRINE MINHO, CPPSE; MARIA ISABEL BOTELHO VIEIRA, UPF.SOARES, S. M.DOMINGUES, R.GASPAR, E. B.SANTOS, P. A. dosCANUTO, K. M.MINHO, A. P.VIEIRA, M. I. B.2019-05-11T00:36:38Z2019-05-11T00:36:38Z2019-05-0920192020-02-17T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9 p.BMC Veterinary Research, v. 15, n. 99, 25 Mar. 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/110890610.1186/s12917-019-1843-7enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-05-11T00:36:46Zoai:www.alice.cnptia.embrapa.br:doc/1108906Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-05-11T00:36:46falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-05-11T00:36:46Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
In vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus. |
title |
In vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus. |
spellingShingle |
In vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus. SOARES, S. M. Maria Mole Planta Tóxica Extrato Vegetal Anti-Helmíntico Nematóide |
title_short |
In vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus. |
title_full |
In vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus. |
title_fullStr |
In vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus. |
title_full_unstemmed |
In vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus. |
title_sort |
In vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus. |
author |
SOARES, S. M. |
author_facet |
SOARES, S. M. DOMINGUES, R. GASPAR, E. B. SANTOS, P. A. dos CANUTO, K. M. MINHO, A. P. VIEIRA, M. I. B. |
author_role |
author |
author2 |
DOMINGUES, R. GASPAR, E. B. SANTOS, P. A. dos CANUTO, K. M. MINHO, A. P. VIEIRA, M. I. B. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
SUELEN MENDONÇA SOARES, UPF; ROBERT DOMINGUES, CPPSUL; EMANUELLE BALDO GASPAR, CPPSUL; PATRÍCIO AZEVEDO DOS SANTOS, URCAMP; KIRLEY MARQUES CANUTO, CNPAT; ALESSANDRO PELEGRINE MINHO, CPPSE; MARIA ISABEL BOTELHO VIEIRA, UPF. |
dc.contributor.author.fl_str_mv |
SOARES, S. M. DOMINGUES, R. GASPAR, E. B. SANTOS, P. A. dos CANUTO, K. M. MINHO, A. P. VIEIRA, M. I. B. |
dc.subject.por.fl_str_mv |
Maria Mole Planta Tóxica Extrato Vegetal Anti-Helmíntico Nematóide |
topic |
Maria Mole Planta Tóxica Extrato Vegetal Anti-Helmíntico Nematóide |
description |
Background: Haemonchosis affects sheep husbandry and its treatment is often compromised due to the development of anthelminthic resistance. Plant-derived bioactive compounds have been studied as alternative to control Haemonchus contortus. The objective of this study was to evaluate the effect of Senecio brasiliensis extracts on H. contortus egg hatching and infective larvae migration. Results: The aqueous extract from dried and fresh plant and alkaloid-enriched fraction of the previously dried leaves of S. brasiliensis inhibited H. contortus egg hatching. The main plant compound in alkaloid fraction was integerrimine, a pyrrolizidine alkaloid (PA). However, the aqueous extract from dried plant displayed higher efficacy when compared to their alkaloid enriched or non-polar fractions, meaning that, although PAs contributed to the ovicidal effect, other compounds in the plant can also contribute to their effect. Furthermore, the aqueous extract from dried plant also had higher efficacy than aqueous extract from fresh plant in larvae migration inhibition. Finally, extract from dried plant presented low in vitro cytotoxic effect. Conclusion: Taken together our results suggest a good anthelmintic effect of S. brasiliensis, especially when aqueous extract is prepared from dried plant. Further in vivo studies should be performed focused on forms of administration of this extract in rearing sheep. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05-11T00:36:38Z 2019-05-11T00:36:38Z 2019-05-09 2019 2020-02-17T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BMC Veterinary Research, v. 15, n. 99, 25 Mar. 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108906 10.1186/s12917-019-1843-7 |
identifier_str_mv |
BMC Veterinary Research, v. 15, n. 99, 25 Mar. 2019. 10.1186/s12917-019-1843-7 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108906 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 p. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503475056345088 |