In vitro conservation of 'Florida Rough' lemon plants.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149407 https://doi.org/10.1590/0103-8478cr20210276 |
Resumo: | The establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of ?Florida Rough? lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L-1 of sucrose, solidified with 7 g.L-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the ?Florida Rough? lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L-1 of sucrose yielded the best ?Florida Rough? lemon plant growth reduction in vitro while maintaining the physiologically health of the plants. |
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In vitro conservation of 'Florida Rough' lemon plants.LimãoCultura In VitroThe establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of ?Florida Rough? lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L-1 of sucrose, solidified with 7 g.L-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the ?Florida Rough? lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L-1 of sucrose yielded the best ?Florida Rough? lemon plant growth reduction in vitro while maintaining the physiologically health of the plants.MARIANE DE JESUS DA SILVA DE CARVALHO, UNIVERSIDADE FEDERAL DO RECÔNCAVO DA BAHIA; ANTONIO DA SILVA SOUZA, CNPMF; EMANUELA BARBOSA SANTOS, UNIVERSIDADE FEDERAL DO CEARÁ; WALTER DOS SANTOS SOARES FILHO, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; EVA MARIA RODRIGUES COSTA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF.CARVALHO, M. de J. da S. deSOUZA, A. da S.EMANUELA BARBOSA SANTOSSOARES FILHO, W. dos S.LEDO, C. A. da S.COSTA, E. M. R.SOUZA, F. V. D.2022-12-08T21:01:20Z2022-12-08T21:01:20Z2022-12-082022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCiência Rural, Santa Maria, v.52:12, e20210276, 2022.1678-4596http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149407https://doi.org/10.1590/0103-8478cr20210276enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-12-08T21:01:20Zoai:www.alice.cnptia.embrapa.br:doc/1149407Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-12-08T21:01:20falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-12-08T21:01:20Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
In vitro conservation of 'Florida Rough' lemon plants. |
title |
In vitro conservation of 'Florida Rough' lemon plants. |
spellingShingle |
In vitro conservation of 'Florida Rough' lemon plants. CARVALHO, M. de J. da S. de Limão Cultura In Vitro |
title_short |
In vitro conservation of 'Florida Rough' lemon plants. |
title_full |
In vitro conservation of 'Florida Rough' lemon plants. |
title_fullStr |
In vitro conservation of 'Florida Rough' lemon plants. |
title_full_unstemmed |
In vitro conservation of 'Florida Rough' lemon plants. |
title_sort |
In vitro conservation of 'Florida Rough' lemon plants. |
author |
CARVALHO, M. de J. da S. de |
author_facet |
CARVALHO, M. de J. da S. de SOUZA, A. da S. EMANUELA BARBOSA SANTOS SOARES FILHO, W. dos S. LEDO, C. A. da S. COSTA, E. M. R. SOUZA, F. V. D. |
author_role |
author |
author2 |
SOUZA, A. da S. EMANUELA BARBOSA SANTOS SOARES FILHO, W. dos S. LEDO, C. A. da S. COSTA, E. M. R. SOUZA, F. V. D. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
MARIANE DE JESUS DA SILVA DE CARVALHO, UNIVERSIDADE FEDERAL DO RECÔNCAVO DA BAHIA; ANTONIO DA SILVA SOUZA, CNPMF; EMANUELA BARBOSA SANTOS, UNIVERSIDADE FEDERAL DO CEARÁ; WALTER DOS SANTOS SOARES FILHO, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; EVA MARIA RODRIGUES COSTA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
dc.contributor.author.fl_str_mv |
CARVALHO, M. de J. da S. de SOUZA, A. da S. EMANUELA BARBOSA SANTOS SOARES FILHO, W. dos S. LEDO, C. A. da S. COSTA, E. M. R. SOUZA, F. V. D. |
dc.subject.por.fl_str_mv |
Limão Cultura In Vitro |
topic |
Limão Cultura In Vitro |
description |
The establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of ?Florida Rough? lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L-1 of sucrose, solidified with 7 g.L-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the ?Florida Rough? lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L-1 of sucrose yielded the best ?Florida Rough? lemon plant growth reduction in vitro while maintaining the physiologically health of the plants. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-08T21:01:20Z 2022-12-08T21:01:20Z 2022-12-08 2022 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Ciência Rural, Santa Maria, v.52:12, e20210276, 2022. 1678-4596 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149407 https://doi.org/10.1590/0103-8478cr20210276 |
identifier_str_mv |
Ciência Rural, Santa Maria, v.52:12, e20210276, 2022. 1678-4596 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149407 https://doi.org/10.1590/0103-8478cr20210276 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503535676620800 |