In vitro conservation of 'Florida Rough' lemon plants.

Detalhes bibliográficos
Autor(a) principal: CARVALHO, M. de J. da S. de
Data de Publicação: 2022
Outros Autores: SOUZA, A. da S., EMANUELA BARBOSA SANTOS, SOARES FILHO, W. dos S., LEDO, C. A. da S., COSTA, E. M. R., SOUZA, F. V. D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149407
https://doi.org/10.1590/0103-8478cr20210276
Resumo: The establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of ?Florida Rough? lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L-1 of sucrose, solidified with 7 g.L-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the ?Florida Rough? lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L-1 of sucrose yielded the best ?Florida Rough? lemon plant growth reduction in vitro while maintaining the physiologically health of the plants.
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spelling In vitro conservation of 'Florida Rough' lemon plants.LimãoCultura In VitroThe establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of ?Florida Rough? lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L-1 of sucrose, solidified with 7 g.L-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the ?Florida Rough? lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L-1 of sucrose yielded the best ?Florida Rough? lemon plant growth reduction in vitro while maintaining the physiologically health of the plants.MARIANE DE JESUS DA SILVA DE CARVALHO, UNIVERSIDADE FEDERAL DO RECÔNCAVO DA BAHIA; ANTONIO DA SILVA SOUZA, CNPMF; EMANUELA BARBOSA SANTOS, UNIVERSIDADE FEDERAL DO CEARÁ; WALTER DOS SANTOS SOARES FILHO, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; EVA MARIA RODRIGUES COSTA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF.CARVALHO, M. de J. da S. deSOUZA, A. da S.EMANUELA BARBOSA SANTOSSOARES FILHO, W. dos S.LEDO, C. A. da S.COSTA, E. M. R.SOUZA, F. V. D.2022-12-08T21:01:20Z2022-12-08T21:01:20Z2022-12-082022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCiência Rural, Santa Maria, v.52:12, e20210276, 2022.1678-4596http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149407https://doi.org/10.1590/0103-8478cr20210276enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-12-08T21:01:20Zoai:www.alice.cnptia.embrapa.br:doc/1149407Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-12-08T21:01:20falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-12-08T21:01:20Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv In vitro conservation of 'Florida Rough' lemon plants.
title In vitro conservation of 'Florida Rough' lemon plants.
spellingShingle In vitro conservation of 'Florida Rough' lemon plants.
CARVALHO, M. de J. da S. de
Limão
Cultura In Vitro
title_short In vitro conservation of 'Florida Rough' lemon plants.
title_full In vitro conservation of 'Florida Rough' lemon plants.
title_fullStr In vitro conservation of 'Florida Rough' lemon plants.
title_full_unstemmed In vitro conservation of 'Florida Rough' lemon plants.
title_sort In vitro conservation of 'Florida Rough' lemon plants.
author CARVALHO, M. de J. da S. de
author_facet CARVALHO, M. de J. da S. de
SOUZA, A. da S.
EMANUELA BARBOSA SANTOS
SOARES FILHO, W. dos S.
LEDO, C. A. da S.
COSTA, E. M. R.
SOUZA, F. V. D.
author_role author
author2 SOUZA, A. da S.
EMANUELA BARBOSA SANTOS
SOARES FILHO, W. dos S.
LEDO, C. A. da S.
COSTA, E. M. R.
SOUZA, F. V. D.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv MARIANE DE JESUS DA SILVA DE CARVALHO, UNIVERSIDADE FEDERAL DO RECÔNCAVO DA BAHIA; ANTONIO DA SILVA SOUZA, CNPMF; EMANUELA BARBOSA SANTOS, UNIVERSIDADE FEDERAL DO CEARÁ; WALTER DOS SANTOS SOARES FILHO, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; EVA MARIA RODRIGUES COSTA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF.
dc.contributor.author.fl_str_mv CARVALHO, M. de J. da S. de
SOUZA, A. da S.
EMANUELA BARBOSA SANTOS
SOARES FILHO, W. dos S.
LEDO, C. A. da S.
COSTA, E. M. R.
SOUZA, F. V. D.
dc.subject.por.fl_str_mv Limão
Cultura In Vitro
topic Limão
Cultura In Vitro
description The establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of ?Florida Rough? lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L-1 of sucrose, solidified with 7 g.L-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the ?Florida Rough? lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L-1 of sucrose yielded the best ?Florida Rough? lemon plant growth reduction in vitro while maintaining the physiologically health of the plants.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-08T21:01:20Z
2022-12-08T21:01:20Z
2022-12-08
2022
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Ciência Rural, Santa Maria, v.52:12, e20210276, 2022.
1678-4596
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149407
https://doi.org/10.1590/0103-8478cr20210276
identifier_str_mv Ciência Rural, Santa Maria, v.52:12, e20210276, 2022.
1678-4596
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149407
https://doi.org/10.1590/0103-8478cr20210276
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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