Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1067524 |
Resumo: | Measures to control maize white spot (MWS) caused by Pantoea ananatis are preferentially based on resistant cultivars. A lack of knowledge on the genetic variability of pathogens could interfere with the development and utilization of controlling strategies in this pathosystem. The main goals of this study were to investigate the genetic variability of 90 P. ananatis isolates from three different eco-geographical regions of Brazil by amplified fragment length polymorphism (AFLP), and to determine the presence of a universal P. ananatis plasmid in isolates from tropical Brazil. Analysis of genetic similarity by AFLP allowed us to categorize the 90 isolates into two groups. However, no correlation between the collecting sites and genetic groupings was observed. The polymorphism percentage found in P. ananatis ranged between 24.64 and 92.46%, and genetic diversity was calculated to be 0.07-0.09. The analysis of molecular variance showed that 99.18% of genetic variability was within the populations, providing evidence that evolutionary forces were acting on these populations. All P. ananatis isolates showed the P. ananatis universal plasmid (280 or 352 kb). This is the first report on the presence of a universal P. ananatis plasmid from MWS lesions in the tropical area. |
id |
EMBR_586f848868769a35575da72a7618ca7c |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1067524 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers.Bactérias fitopatogênicasGenotipagem de alelosDoença de plantaMilhoPolimorfismoMeasures to control maize white spot (MWS) caused by Pantoea ananatis are preferentially based on resistant cultivars. A lack of knowledge on the genetic variability of pathogens could interfere with the development and utilization of controlling strategies in this pathosystem. The main goals of this study were to investigate the genetic variability of 90 P. ananatis isolates from three different eco-geographical regions of Brazil by amplified fragment length polymorphism (AFLP), and to determine the presence of a universal P. ananatis plasmid in isolates from tropical Brazil. Analysis of genetic similarity by AFLP allowed us to categorize the 90 isolates into two groups. However, no correlation between the collecting sites and genetic groupings was observed. The polymorphism percentage found in P. ananatis ranged between 24.64 and 92.46%, and genetic diversity was calculated to be 0.07-0.09. The analysis of molecular variance showed that 99.18% of genetic variability was within the populations, providing evidence that evolutionary forces were acting on these populations. All P. ananatis isolates showed the P. ananatis universal plasmid (280 or 352 kb). This is the first report on the presence of a universal P. ananatis plasmid from MWS lesions in the tropical area.JOSE EDSON FONTES FIGUEIREDO, CNPMS.MILLER, A. M.FIGUEIREDO, J. E. F.CHAVES, C. L.RUAS, E. A.BALBI-PEÑA, M. I.COLAUTO, N. B.PACCOLA-MEIRELLES, L. D.2017-03-23T11:11:11Z2017-03-23T11:11:11Z2017-03-2320162017-03-28T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, Ribeirão Preto, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/106752410.4238/gmr.15049452enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:19:40Zoai:www.alice.cnptia.embrapa.br:doc/1067524Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:19:40falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:19:40Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers. |
title |
Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers. |
spellingShingle |
Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers. MILLER, A. M. Bactérias fitopatogênicas Genotipagem de alelos Doença de planta Milho Polimorfismo |
title_short |
Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers. |
title_full |
Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers. |
title_fullStr |
Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers. |
title_full_unstemmed |
Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers. |
title_sort |
Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers. |
author |
MILLER, A. M. |
author_facet |
MILLER, A. M. FIGUEIREDO, J. E. F. CHAVES, C. L. RUAS, E. A. BALBI-PEÑA, M. I. COLAUTO, N. B. PACCOLA-MEIRELLES, L. D. |
author_role |
author |
author2 |
FIGUEIREDO, J. E. F. CHAVES, C. L. RUAS, E. A. BALBI-PEÑA, M. I. COLAUTO, N. B. PACCOLA-MEIRELLES, L. D. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
JOSE EDSON FONTES FIGUEIREDO, CNPMS. |
dc.contributor.author.fl_str_mv |
MILLER, A. M. FIGUEIREDO, J. E. F. CHAVES, C. L. RUAS, E. A. BALBI-PEÑA, M. I. COLAUTO, N. B. PACCOLA-MEIRELLES, L. D. |
dc.subject.por.fl_str_mv |
Bactérias fitopatogênicas Genotipagem de alelos Doença de planta Milho Polimorfismo |
topic |
Bactérias fitopatogênicas Genotipagem de alelos Doença de planta Milho Polimorfismo |
description |
Measures to control maize white spot (MWS) caused by Pantoea ananatis are preferentially based on resistant cultivars. A lack of knowledge on the genetic variability of pathogens could interfere with the development and utilization of controlling strategies in this pathosystem. The main goals of this study were to investigate the genetic variability of 90 P. ananatis isolates from three different eco-geographical regions of Brazil by amplified fragment length polymorphism (AFLP), and to determine the presence of a universal P. ananatis plasmid in isolates from tropical Brazil. Analysis of genetic similarity by AFLP allowed us to categorize the 90 isolates into two groups. However, no correlation between the collecting sites and genetic groupings was observed. The polymorphism percentage found in P. ananatis ranged between 24.64 and 92.46%, and genetic diversity was calculated to be 0.07-0.09. The analysis of molecular variance showed that 99.18% of genetic variability was within the populations, providing evidence that evolutionary forces were acting on these populations. All P. ananatis isolates showed the P. ananatis universal plasmid (280 or 352 kb). This is the first report on the presence of a universal P. ananatis plasmid from MWS lesions in the tropical area. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2017-03-23T11:11:11Z 2017-03-23T11:11:11Z 2017-03-23 2017-03-28T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, Ribeirão Preto, 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1067524 10.4238/gmr.15049452 |
identifier_str_mv |
Genetics and Molecular Research, Ribeirão Preto, 2016. 10.4238/gmr.15049452 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1067524 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503434462822400 |