Flavan-3-ol (Flavanol) identification and quantitation by high performance liquid chromatography coupled with mass spectrometry (HPLC-MSn).
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Capítulo de livro |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153794 https://doi.org/10.1007/978-1-0716-3088-4 |
Resumo: | Flavan-3-ols are widely distributed in higher plants, such as grapes, located in the skins and seeds, being transferred to the wine during winemaking. They are responsible for specific sensory properties such as astringency, and bitterness, acting on the stability of the wine color, and taking part in the antioxidant compounds. The interest in identifying flavan-3-ols has grown in the last decades since the development of new instruments allowing a better separation and characterization, both qualitative and quantitative. New isolation, separation, and identification techniques allowed an increase in the phenolic compounds database with new structures, providing a better understanding of the mechanisms involving phenolic metabolism in grapes, wines, and other fruits and vegetables. High-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) is one of the most relevant and used analytical techniques for the non-volatile and/or thermally unstable compounds determination. This method has shown to be valuable and robust for investigating the polyphenols (flavan-3-ols or proanthocyanidins) in grapes, wines, and derivates, in several domains, such as evaluating the effect of climate, soil, vine management, cultivars, rootstocks, protocols of elaboration, and the quality control. This chapter aims to present variations of LC-MS techniques used to identify these compounds in recent years. |
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Flavan-3-ol (Flavanol) identification and quantitation by high performance liquid chromatography coupled with mass spectrometry (HPLC-MSn).Vitis vinifera LGrapesWinesPhenolic compoundsSecondary metabolitesFlavan-3-ols are widely distributed in higher plants, such as grapes, located in the skins and seeds, being transferred to the wine during winemaking. They are responsible for specific sensory properties such as astringency, and bitterness, acting on the stability of the wine color, and taking part in the antioxidant compounds. The interest in identifying flavan-3-ols has grown in the last decades since the development of new instruments allowing a better separation and characterization, both qualitative and quantitative. New isolation, separation, and identification techniques allowed an increase in the phenolic compounds database with new structures, providing a better understanding of the mechanisms involving phenolic metabolism in grapes, wines, and other fruits and vegetables. High-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) is one of the most relevant and used analytical techniques for the non-volatile and/or thermally unstable compounds determination. This method has shown to be valuable and robust for investigating the polyphenols (flavan-3-ols or proanthocyanidins) in grapes, wines, and derivates, in several domains, such as evaluating the effect of climate, soil, vine management, cultivars, rootstocks, protocols of elaboration, and the quality control. This chapter aims to present variations of LC-MS techniques used to identify these compounds in recent years.JULIANE BARRETO DE OLIVEIRA, MINAS GERAIS AGRICULTURAL RESEARCH CORPORATION; CELSO GUARANI RUIZ DE OLIVEIRA, CNPUV; GIULIANO ELIAS PEREIRA, CNPUV.OLIVEIRA, J. B. DEOLIVEIRA, C. G. R. dePEREIRA, G. E.2023-05-17T19:48:29Z2023-05-17T19:48:29Z2023-05-172023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPartp. 86-100In: CASTILHOS, M. B. M. DE (Ed.). Basic protocols in enology an winemaking. Campinas: Unicamp; New York:Springer Nature, 2023. Cap. 8.978-1-0716-3088-42662-9518http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153794https://doi.org/10.1007/978-1-0716-3088-4eng(Springer Protocols. Methods and Protocols in Food Science)info:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-05-17T19:48:29Zoai:www.alice.cnptia.embrapa.br:doc/1153794Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-05-17T19:48:29falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-05-17T19:48:29Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Flavan-3-ol (Flavanol) identification and quantitation by high performance liquid chromatography coupled with mass spectrometry (HPLC-MSn). |
title |
Flavan-3-ol (Flavanol) identification and quantitation by high performance liquid chromatography coupled with mass spectrometry (HPLC-MSn). |
spellingShingle |
Flavan-3-ol (Flavanol) identification and quantitation by high performance liquid chromatography coupled with mass spectrometry (HPLC-MSn). OLIVEIRA, J. B. DE Vitis vinifera L Grapes Wines Phenolic compounds Secondary metabolites |
title_short |
Flavan-3-ol (Flavanol) identification and quantitation by high performance liquid chromatography coupled with mass spectrometry (HPLC-MSn). |
title_full |
Flavan-3-ol (Flavanol) identification and quantitation by high performance liquid chromatography coupled with mass spectrometry (HPLC-MSn). |
title_fullStr |
Flavan-3-ol (Flavanol) identification and quantitation by high performance liquid chromatography coupled with mass spectrometry (HPLC-MSn). |
title_full_unstemmed |
Flavan-3-ol (Flavanol) identification and quantitation by high performance liquid chromatography coupled with mass spectrometry (HPLC-MSn). |
title_sort |
Flavan-3-ol (Flavanol) identification and quantitation by high performance liquid chromatography coupled with mass spectrometry (HPLC-MSn). |
author |
OLIVEIRA, J. B. DE |
author_facet |
OLIVEIRA, J. B. DE OLIVEIRA, C. G. R. de PEREIRA, G. E. |
author_role |
author |
author2 |
OLIVEIRA, C. G. R. de PEREIRA, G. E. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
JULIANE BARRETO DE OLIVEIRA, MINAS GERAIS AGRICULTURAL RESEARCH CORPORATION; CELSO GUARANI RUIZ DE OLIVEIRA, CNPUV; GIULIANO ELIAS PEREIRA, CNPUV. |
dc.contributor.author.fl_str_mv |
OLIVEIRA, J. B. DE OLIVEIRA, C. G. R. de PEREIRA, G. E. |
dc.subject.por.fl_str_mv |
Vitis vinifera L Grapes Wines Phenolic compounds Secondary metabolites |
topic |
Vitis vinifera L Grapes Wines Phenolic compounds Secondary metabolites |
description |
Flavan-3-ols are widely distributed in higher plants, such as grapes, located in the skins and seeds, being transferred to the wine during winemaking. They are responsible for specific sensory properties such as astringency, and bitterness, acting on the stability of the wine color, and taking part in the antioxidant compounds. The interest in identifying flavan-3-ols has grown in the last decades since the development of new instruments allowing a better separation and characterization, both qualitative and quantitative. New isolation, separation, and identification techniques allowed an increase in the phenolic compounds database with new structures, providing a better understanding of the mechanisms involving phenolic metabolism in grapes, wines, and other fruits and vegetables. High-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) is one of the most relevant and used analytical techniques for the non-volatile and/or thermally unstable compounds determination. This method has shown to be valuable and robust for investigating the polyphenols (flavan-3-ols or proanthocyanidins) in grapes, wines, and derivates, in several domains, such as evaluating the effect of climate, soil, vine management, cultivars, rootstocks, protocols of elaboration, and the quality control. This chapter aims to present variations of LC-MS techniques used to identify these compounds in recent years. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-17T19:48:29Z 2023-05-17T19:48:29Z 2023-05-17 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bookPart |
format |
bookPart |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
In: CASTILHOS, M. B. M. DE (Ed.). Basic protocols in enology an winemaking. Campinas: Unicamp; New York:Springer Nature, 2023. Cap. 8. 978-1-0716-3088-4 2662-9518 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153794 https://doi.org/10.1007/978-1-0716-3088-4 |
identifier_str_mv |
In: CASTILHOS, M. B. M. DE (Ed.). Basic protocols in enology an winemaking. Campinas: Unicamp; New York:Springer Nature, 2023. Cap. 8. 978-1-0716-3088-4 2662-9518 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153794 https://doi.org/10.1007/978-1-0716-3088-4 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
(Springer Protocols. Methods and Protocols in Food Science) |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
p. 86-100 |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503545079201792 |