Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144832 |
Resumo: | The cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures. |
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Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease.Enzyme-linked immunosorbent assay (ELISA)CLRDVIntermediary hostVírusSerologyThe cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures.LUCIA VIEIRA HOFFMANN, CNPA; AMANDA ALVES BRANQUINHO, UNIVERSIDADE FEDERAL DE GOIÁS; PAULO AUGUSTO VIANNA BARROSO, CNPM; MAITE F. S. VASLIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO.HOFFMANN, L. V.BRANQUINHO, A. A.BARROSO, P. A. V.VASLIN, M. F. S.2022-07-20T17:30:04Z2022-07-20T17:30:04Z2022-07-202022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFrontiers in Plant Science, v. 13, n. 814119, 5 p. 2022.1664-462Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/114483210.3389/fpls.2022.814119enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-07-20T17:30:13Zoai:www.alice.cnptia.embrapa.br:doc/1144832Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-07-20T17:30:13falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-07-20T17:30:13Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease. |
title |
Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease. |
spellingShingle |
Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease. HOFFMANN, L. V. Enzyme-linked immunosorbent assay (ELISA) CLRDV Intermediary host Vírus Serology |
title_short |
Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease. |
title_full |
Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease. |
title_fullStr |
Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease. |
title_full_unstemmed |
Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease. |
title_sort |
Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease. |
author |
HOFFMANN, L. V. |
author_facet |
HOFFMANN, L. V. BRANQUINHO, A. A. BARROSO, P. A. V. VASLIN, M. F. S. |
author_role |
author |
author2 |
BRANQUINHO, A. A. BARROSO, P. A. V. VASLIN, M. F. S. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
LUCIA VIEIRA HOFFMANN, CNPA; AMANDA ALVES BRANQUINHO, UNIVERSIDADE FEDERAL DE GOIÁS; PAULO AUGUSTO VIANNA BARROSO, CNPM; MAITE F. S. VASLIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
dc.contributor.author.fl_str_mv |
HOFFMANN, L. V. BRANQUINHO, A. A. BARROSO, P. A. V. VASLIN, M. F. S. |
dc.subject.por.fl_str_mv |
Enzyme-linked immunosorbent assay (ELISA) CLRDV Intermediary host Vírus Serology |
topic |
Enzyme-linked immunosorbent assay (ELISA) CLRDV Intermediary host Vírus Serology |
description |
The cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-20T17:30:04Z 2022-07-20T17:30:04Z 2022-07-20 2022 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Frontiers in Plant Science, v. 13, n. 814119, 5 p. 2022. 1664-462X http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144832 10.3389/fpls.2022.814119 |
identifier_str_mv |
Frontiers in Plant Science, v. 13, n. 814119, 5 p. 2022. 1664-462X 10.3389/fpls.2022.814119 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144832 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503526632652800 |