Bioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera: Tephritidae).
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102300 |
Resumo: | The development of appropriate methodologies for bioassays is of paramount importance to study the methodological factors that may interfere in the experimental design. Thus, laboratory bioassays have become increasingly important for the determination of data that are realistic and replicable. The objective of the study was to develop a bioassay methodology with the ready-to-use toxic bait Success ? 0.02CB, 96 mg L−1 of spinosad, in adults of Ceratitis capitata (Wiedemann) as a study model. The insect origin laboratory population, LT 50= 11.16 h), time of toxic bait supply (4 h, LT 50= 16.85 h), food deprivation (12 h, LT 50= 15.55 h), and the adult age (5 days, LT 50= 35.31 h) of C. capitata showed higher susceptibility and a shorter LT 50 lethal time to the Success? 0.02CB toxic bait. However, the absence or presence of a food source (LT 50≈31.17 h) during the bioassay did not affect the susceptibility of C. capitata. Further, adults of C. capitata who were deprived and in the absence of a food source (artificial diet) had higher consumption of toxic baits (mg) during treatment exposure. The use of a 12-h deprivation period, toxic baits offered for 4 h, and absence of an artificial diet for adults of C. capitata at 5 days of age during the bioassay are considered adequate for evaluating formulations of toxic baits under laboratory conditions. Keywords: Toxicity, methodology, spinosad, Mediterranean fruit fly |
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Bioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera: Tephritidae).Mediterranean fruit flyToxicityMethodologySpinosadThe development of appropriate methodologies for bioassays is of paramount importance to study the methodological factors that may interfere in the experimental design. Thus, laboratory bioassays have become increasingly important for the determination of data that are realistic and replicable. The objective of the study was to develop a bioassay methodology with the ready-to-use toxic bait Success ? 0.02CB, 96 mg L−1 of spinosad, in adults of Ceratitis capitata (Wiedemann) as a study model. The insect origin laboratory population, LT 50= 11.16 h), time of toxic bait supply (4 h, LT 50= 16.85 h), food deprivation (12 h, LT 50= 15.55 h), and the adult age (5 days, LT 50= 35.31 h) of C. capitata showed higher susceptibility and a shorter LT 50 lethal time to the Success? 0.02CB toxic bait. However, the absence or presence of a food source (LT 50≈31.17 h) during the bioassay did not affect the susceptibility of C. capitata. Further, adults of C. capitata who were deprived and in the absence of a food source (artificial diet) had higher consumption of toxic baits (mg) during treatment exposure. The use of a 12-h deprivation period, toxic baits offered for 4 h, and absence of an artificial diet for adults of C. capitata at 5 days of age during the bioassay are considered adequate for evaluating formulations of toxic baits under laboratory conditions. Keywords: Toxicity, methodology, spinosad, Mediterranean fruit flyCléber Antônio Barônio, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, BrasilDaniel Bernardi, Dept of Entomology, Embrapa Clima Temperado, Pelotas, RS, BrasilMarcelo Z. Nunes, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, BrasiLJ. Pasinato, Embrapa Uva e Vinho, Bento Gonçalves, Rio Grande do Sul, BrasilF. R. M. Garcia, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, BrasilMARCOS BOTTON, CNPUV.BARÔNIO, C. A.BERNARDI, D.NUNES, M. Z.PASINATO, J.GARCIA, F. R. M.BOTTON, M.2018-12-23T23:32:34Z2018-12-23T23:32:34Z2018-12-2020182019-04-27T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNeotropical Entomology, [online], dec. 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/110230010.1007/s13744-018-0653-0enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-12-23T23:32:40Zoai:www.alice.cnptia.embrapa.br:doc/1102300Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-12-23T23:32:40falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-12-23T23:32:40Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Bioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera: Tephritidae). |
title |
Bioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera: Tephritidae). |
spellingShingle |
Bioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera: Tephritidae). BARÔNIO, C. A. Mediterranean fruit fly Toxicity Methodology Spinosad |
title_short |
Bioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera: Tephritidae). |
title_full |
Bioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera: Tephritidae). |
title_fullStr |
Bioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera: Tephritidae). |
title_full_unstemmed |
Bioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera: Tephritidae). |
title_sort |
Bioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera: Tephritidae). |
author |
BARÔNIO, C. A. |
author_facet |
BARÔNIO, C. A. BERNARDI, D. NUNES, M. Z. PASINATO, J. GARCIA, F. R. M. BOTTON, M. |
author_role |
author |
author2 |
BERNARDI, D. NUNES, M. Z. PASINATO, J. GARCIA, F. R. M. BOTTON, M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Cléber Antônio Barônio, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, Brasil Daniel Bernardi, Dept of Entomology, Embrapa Clima Temperado, Pelotas, RS, Brasil Marcelo Z. Nunes, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, BrasiL J. Pasinato, Embrapa Uva e Vinho, Bento Gonçalves, Rio Grande do Sul, Brasil F. R. M. Garcia, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, Brasil MARCOS BOTTON, CNPUV. |
dc.contributor.author.fl_str_mv |
BARÔNIO, C. A. BERNARDI, D. NUNES, M. Z. PASINATO, J. GARCIA, F. R. M. BOTTON, M. |
dc.subject.por.fl_str_mv |
Mediterranean fruit fly Toxicity Methodology Spinosad |
topic |
Mediterranean fruit fly Toxicity Methodology Spinosad |
description |
The development of appropriate methodologies for bioassays is of paramount importance to study the methodological factors that may interfere in the experimental design. Thus, laboratory bioassays have become increasingly important for the determination of data that are realistic and replicable. The objective of the study was to develop a bioassay methodology with the ready-to-use toxic bait Success ? 0.02CB, 96 mg L−1 of spinosad, in adults of Ceratitis capitata (Wiedemann) as a study model. The insect origin laboratory population, LT 50= 11.16 h), time of toxic bait supply (4 h, LT 50= 16.85 h), food deprivation (12 h, LT 50= 15.55 h), and the adult age (5 days, LT 50= 35.31 h) of C. capitata showed higher susceptibility and a shorter LT 50 lethal time to the Success? 0.02CB toxic bait. However, the absence or presence of a food source (LT 50≈31.17 h) during the bioassay did not affect the susceptibility of C. capitata. Further, adults of C. capitata who were deprived and in the absence of a food source (artificial diet) had higher consumption of toxic baits (mg) during treatment exposure. The use of a 12-h deprivation period, toxic baits offered for 4 h, and absence of an artificial diet for adults of C. capitata at 5 days of age during the bioassay are considered adequate for evaluating formulations of toxic baits under laboratory conditions. Keywords: Toxicity, methodology, spinosad, Mediterranean fruit fly |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-23T23:32:34Z 2018-12-23T23:32:34Z 2018-12-20 2018 2019-04-27T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Neotropical Entomology, [online], dec. 2018. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102300 10.1007/s13744-018-0653-0 |
identifier_str_mv |
Neotropical Entomology, [online], dec. 2018. 10.1007/s13744-018-0653-0 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102300 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503467862065152 |