Diversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus) evaluated by multiple extraction methods.

• Autor(a) principal: MOREIRA, R. F.
• Data de Publicação: 2018
• Outros Autores: MATOS, M. N. C., ALVES FILHO, J. G., VALLE, R. V. do, ELOY, A. M. X., PINTO, T. M. F., MACHADO, S. P., COSTA, C. R. R., LIMA FILHO, J. L. de, LIMA, J. P. M. S., CUNHA, R. M. S. da
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1090515
• topic: Isolation methods; Proteomic profiles; Proteomic analysis; Raça Moxotó; Análise proteômica; Caprino; Detergente; Goats; Surfactants; Spermatozoa; detergents; proteomics

Abstract: This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1% or 4% CHAPS; 1% or 4% SDS; 1% or 4% Triton X-100; or a combination of CHAPS and SDS. The 1-DE and 2-DE profiles of the isolated proteins revealed that the various isolation methods were efficient. Qualitative and quantitative differences in the 1-DE and 2-DE profiles were observed. 2-DE maps indicated that the amount and diversity of proteins visualized depended on the detergent that was used. Furthermore, this work revealed that the combination of detergents increased the resolution of some spots and retained the characteristics of the individual detergents, depending on their concentrations.