Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115412 https://doi.org/10.3389/fmicb.2019.02625 |
Resumo: | Abstract: Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture-independent-based method. Although culture-independent methods provide a higher resolution of analysis, it was found that in the rhizosphere, particular operational taxonomic units (OTUs) in the culture-dependent fraction were absent from the culture-independent fraction, indicating that deeper sequence analysis is required for this approach to be exhaustive. We also found that the microbial seed load defined the endosphere, but not rhizosphere, community structure for plants grown in soil which was not wheat adapted. Together, these findings increase our understanding of the importance of land management and microbial seed load in shaping the root microbiome of wheat and this knowledge will facilitate the exploitation of plant?microbe interactions for the development of novel microbial inoculants. |
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Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat.EndosphereRizosferaPopulação MicrobianaManejo do SoloTrigoSementeRhizosphereMicrobiomeWheatSeedsAbstract: Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture-independent-based method. Although culture-independent methods provide a higher resolution of analysis, it was found that in the rhizosphere, particular operational taxonomic units (OTUs) in the culture-dependent fraction were absent from the culture-independent fraction, indicating that deeper sequence analysis is required for this approach to be exhaustive. We also found that the microbial seed load defined the endosphere, but not rhizosphere, community structure for plants grown in soil which was not wheat adapted. Together, these findings increase our understanding of the importance of land management and microbial seed load in shaping the root microbiome of wheat and this knowledge will facilitate the exploitation of plant?microbe interactions for the development of novel microbial inoculants.VANESSA NESSNER KAVAMURA, Rothamsted Research; REBEKAH J ROBINSON, Plant Pathology Laboratory, RHS; RIFAT HAYAT, Pir Mehr Ali Shah Arid Agriculture University; IAN MICHAEL CLARK, Rothamsted Research; DAVID HUGHES, Rothamsted Research; MAIKE ROSSMANN; PENNY R HIRSCH, Rothamsted Research; RODRIGO MENDES, CNPMA; TIM H MAUCHLINE, Rothamsted Research.KAVAMURA, V. N.ROBINSON, R. J.HAYAT, R.CLARK, I. M.HUGHES, D.ROSSMANN, M.HIRSCH, P. R.MENDES, R.MAUCHLINE, T. H.2019-11-28T18:09:03Z2019-11-28T18:09:03Z2019-11-2820192019-11-28T18:09:03Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFrontiers in Microbiology, Lausanne, v. 10, 2019. Article 2625.1664-302Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1115412https://doi.org/10.3389/fmicb.2019.02625enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-11-28T18:09:09Zoai:www.alice.cnptia.embrapa.br:doc/1115412Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-11-28T18:09:09falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-11-28T18:09:09Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat. |
title |
Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat. |
spellingShingle |
Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat. KAVAMURA, V. N. Endosphere Rizosfera População Microbiana Manejo do Solo Trigo Semente Rhizosphere Microbiome Wheat Seeds |
title_short |
Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat. |
title_full |
Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat. |
title_fullStr |
Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat. |
title_full_unstemmed |
Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat. |
title_sort |
Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat. |
author |
KAVAMURA, V. N. |
author_facet |
KAVAMURA, V. N. ROBINSON, R. J. HAYAT, R. CLARK, I. M. HUGHES, D. ROSSMANN, M. HIRSCH, P. R. MENDES, R. MAUCHLINE, T. H. |
author_role |
author |
author2 |
ROBINSON, R. J. HAYAT, R. CLARK, I. M. HUGHES, D. ROSSMANN, M. HIRSCH, P. R. MENDES, R. MAUCHLINE, T. H. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
VANESSA NESSNER KAVAMURA, Rothamsted Research; REBEKAH J ROBINSON, Plant Pathology Laboratory, RHS; RIFAT HAYAT, Pir Mehr Ali Shah Arid Agriculture University; IAN MICHAEL CLARK, Rothamsted Research; DAVID HUGHES, Rothamsted Research; MAIKE ROSSMANN; PENNY R HIRSCH, Rothamsted Research; RODRIGO MENDES, CNPMA; TIM H MAUCHLINE, Rothamsted Research. |
dc.contributor.author.fl_str_mv |
KAVAMURA, V. N. ROBINSON, R. J. HAYAT, R. CLARK, I. M. HUGHES, D. ROSSMANN, M. HIRSCH, P. R. MENDES, R. MAUCHLINE, T. H. |
dc.subject.por.fl_str_mv |
Endosphere Rizosfera População Microbiana Manejo do Solo Trigo Semente Rhizosphere Microbiome Wheat Seeds |
topic |
Endosphere Rizosfera População Microbiana Manejo do Solo Trigo Semente Rhizosphere Microbiome Wheat Seeds |
description |
Abstract: Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture-independent-based method. Although culture-independent methods provide a higher resolution of analysis, it was found that in the rhizosphere, particular operational taxonomic units (OTUs) in the culture-dependent fraction were absent from the culture-independent fraction, indicating that deeper sequence analysis is required for this approach to be exhaustive. We also found that the microbial seed load defined the endosphere, but not rhizosphere, community structure for plants grown in soil which was not wheat adapted. Together, these findings increase our understanding of the importance of land management and microbial seed load in shaping the root microbiome of wheat and this knowledge will facilitate the exploitation of plant?microbe interactions for the development of novel microbial inoculants. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-28T18:09:03Z 2019-11-28T18:09:03Z 2019-11-28 2019 2019-11-28T18:09:03Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Frontiers in Microbiology, Lausanne, v. 10, 2019. Article 2625. 1664-302X http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115412 https://doi.org/10.3389/fmicb.2019.02625 |
identifier_str_mv |
Frontiers in Microbiology, Lausanne, v. 10, 2019. Article 2625. 1664-302X |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115412 https://doi.org/10.3389/fmicb.2019.02625 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503484567977984 |