Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat.

Detalhes bibliográficos
Autor(a) principal: KAVAMURA, V. N.
Data de Publicação: 2019
Outros Autores: ROBINSON, R. J., HAYAT, R., CLARK, I. M., HUGHES, D., ROSSMANN, M., HIRSCH, P. R., MENDES, R., MAUCHLINE, T. H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115412
https://doi.org/10.3389/fmicb.2019.02625
Resumo: Abstract: Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture-independent-based method. Although culture-independent methods provide a higher resolution of analysis, it was found that in the rhizosphere, particular operational taxonomic units (OTUs) in the culture-dependent fraction were absent from the culture-independent fraction, indicating that deeper sequence analysis is required for this approach to be exhaustive. We also found that the microbial seed load defined the endosphere, but not rhizosphere, community structure for plants grown in soil which was not wheat adapted. Together, these findings increase our understanding of the importance of land management and microbial seed load in shaping the root microbiome of wheat and this knowledge will facilitate the exploitation of plant?microbe interactions for the development of novel microbial inoculants.
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spelling Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat.EndosphereRizosferaPopulação MicrobianaManejo do SoloTrigoSementeRhizosphereMicrobiomeWheatSeedsAbstract: Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture-independent-based method. Although culture-independent methods provide a higher resolution of analysis, it was found that in the rhizosphere, particular operational taxonomic units (OTUs) in the culture-dependent fraction were absent from the culture-independent fraction, indicating that deeper sequence analysis is required for this approach to be exhaustive. We also found that the microbial seed load defined the endosphere, but not rhizosphere, community structure for plants grown in soil which was not wheat adapted. Together, these findings increase our understanding of the importance of land management and microbial seed load in shaping the root microbiome of wheat and this knowledge will facilitate the exploitation of plant?microbe interactions for the development of novel microbial inoculants.VANESSA NESSNER KAVAMURA, Rothamsted Research; REBEKAH J ROBINSON, Plant Pathology Laboratory, RHS; RIFAT HAYAT, Pir Mehr Ali Shah Arid Agriculture University; IAN MICHAEL CLARK, Rothamsted Research; DAVID HUGHES, Rothamsted Research; MAIKE ROSSMANN; PENNY R HIRSCH, Rothamsted Research; RODRIGO MENDES, CNPMA; TIM H MAUCHLINE, Rothamsted Research.KAVAMURA, V. N.ROBINSON, R. J.HAYAT, R.CLARK, I. M.HUGHES, D.ROSSMANN, M.HIRSCH, P. R.MENDES, R.MAUCHLINE, T. H.2019-11-28T18:09:03Z2019-11-28T18:09:03Z2019-11-2820192019-11-28T18:09:03Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFrontiers in Microbiology, Lausanne, v. 10, 2019. Article 2625.1664-302Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1115412https://doi.org/10.3389/fmicb.2019.02625enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-11-28T18:09:09Zoai:www.alice.cnptia.embrapa.br:doc/1115412Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-11-28T18:09:09falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-11-28T18:09:09Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat.
title Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat.
spellingShingle Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat.
KAVAMURA, V. N.
Endosphere
Rizosfera
População Microbiana
Manejo do Solo
Trigo
Semente
Rhizosphere
Microbiome
Wheat
Seeds
title_short Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat.
title_full Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat.
title_fullStr Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat.
title_full_unstemmed Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat.
title_sort Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat.
author KAVAMURA, V. N.
author_facet KAVAMURA, V. N.
ROBINSON, R. J.
HAYAT, R.
CLARK, I. M.
HUGHES, D.
ROSSMANN, M.
HIRSCH, P. R.
MENDES, R.
MAUCHLINE, T. H.
author_role author
author2 ROBINSON, R. J.
HAYAT, R.
CLARK, I. M.
HUGHES, D.
ROSSMANN, M.
HIRSCH, P. R.
MENDES, R.
MAUCHLINE, T. H.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv VANESSA NESSNER KAVAMURA, Rothamsted Research; REBEKAH J ROBINSON, Plant Pathology Laboratory, RHS; RIFAT HAYAT, Pir Mehr Ali Shah Arid Agriculture University; IAN MICHAEL CLARK, Rothamsted Research; DAVID HUGHES, Rothamsted Research; MAIKE ROSSMANN; PENNY R HIRSCH, Rothamsted Research; RODRIGO MENDES, CNPMA; TIM H MAUCHLINE, Rothamsted Research.
dc.contributor.author.fl_str_mv KAVAMURA, V. N.
ROBINSON, R. J.
HAYAT, R.
CLARK, I. M.
HUGHES, D.
ROSSMANN, M.
HIRSCH, P. R.
MENDES, R.
MAUCHLINE, T. H.
dc.subject.por.fl_str_mv Endosphere
Rizosfera
População Microbiana
Manejo do Solo
Trigo
Semente
Rhizosphere
Microbiome
Wheat
Seeds
topic Endosphere
Rizosfera
População Microbiana
Manejo do Solo
Trigo
Semente
Rhizosphere
Microbiome
Wheat
Seeds
description Abstract: Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture-independent-based method. Although culture-independent methods provide a higher resolution of analysis, it was found that in the rhizosphere, particular operational taxonomic units (OTUs) in the culture-dependent fraction were absent from the culture-independent fraction, indicating that deeper sequence analysis is required for this approach to be exhaustive. We also found that the microbial seed load defined the endosphere, but not rhizosphere, community structure for plants grown in soil which was not wheat adapted. Together, these findings increase our understanding of the importance of land management and microbial seed load in shaping the root microbiome of wheat and this knowledge will facilitate the exploitation of plant?microbe interactions for the development of novel microbial inoculants.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-28T18:09:03Z
2019-11-28T18:09:03Z
2019-11-28
2019
2019-11-28T18:09:03Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Frontiers in Microbiology, Lausanne, v. 10, 2019. Article 2625.
1664-302X
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115412
https://doi.org/10.3389/fmicb.2019.02625
identifier_str_mv Frontiers in Microbiology, Lausanne, v. 10, 2019. Article 2625.
1664-302X
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115412
https://doi.org/10.3389/fmicb.2019.02625
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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