Molecular analysis of genetic diversity among vine accessions using DNA markers.

Detalhes bibliográficos
Autor(a) principal: COSTA, A. F. da
Data de Publicação: 2017
Outros Autores: TEODORO, P. E., BHERING, L. L., TARDIN, F. D., DAHER, R. F., CAMPOS, W. F., VIANA, A. P., PEREIRA, M. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082614
Resumo: Viticulture presents a number of economic and social advantages, such as increasing employment levels and fixing the labor force in rural areas. With the aim of initiating a program of genetic improvement in grapevine from the State University of the state of Rio de Janeiro North Darcy Ribeiro, genetic diversity between 40 genotypes (varieties, rootstock, and species of different subgenera) was evaluated using Random amplified polymorphic DNA (RAPD) molecular markers. We built a matrix of binary data, whereby the presence of a band was assigned as "1" and the absence of a band was assigned as "0". The genetic distance was calculated between pairs of genotypes based on the arithmetic complement from the Jaccard Index. The results revealed the presence of considerable variability in the collection. Analysis of the genetic dissimilarity matrix revealed that the most dissimilar genotypes were Rupestris du Lot and Vitis rotundifolia because they were the most genetically distant (0.5972). The most similar were genotypes 31 (unidentified) and Rupestris du lot, which showed zero distance, confirming the results of field observations. A duplicate was confirmed, consistent with field observations, and a short distance was found between the variety "Italy" and its mutation, "Ruby". The grouping methods used were somewhat concordant.
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spelling Molecular analysis of genetic diversity among vine accessions using DNA markers.Análise molecularVideiraMarcador molecularVariação genéticaViticulture presents a number of economic and social advantages, such as increasing employment levels and fixing the labor force in rural areas. With the aim of initiating a program of genetic improvement in grapevine from the State University of the state of Rio de Janeiro North Darcy Ribeiro, genetic diversity between 40 genotypes (varieties, rootstock, and species of different subgenera) was evaluated using Random amplified polymorphic DNA (RAPD) molecular markers. We built a matrix of binary data, whereby the presence of a band was assigned as "1" and the absence of a band was assigned as "0". The genetic distance was calculated between pairs of genotypes based on the arithmetic complement from the Jaccard Index. The results revealed the presence of considerable variability in the collection. Analysis of the genetic dissimilarity matrix revealed that the most dissimilar genotypes were Rupestris du Lot and Vitis rotundifolia because they were the most genetically distant (0.5972). The most similar were genotypes 31 (unidentified) and Rupestris du lot, which showed zero distance, confirming the results of field observations. A duplicate was confirmed, consistent with field observations, and a short distance was found between the variety "Italy" and its mutation, "Ruby". The grouping methods used were somewhat concordant.Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural; Universidade Federal de Viçosa; Universidade Federal de Viçosa; FLAVIO DESSAUNE TARDIN, CNPMS; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Universidade Estadual do Norte Fluminense Darcy Ribeiro.COSTA, A. F. daTEODORO, P. E.BHERING, L. L.TARDIN, F. D.DAHER, R. F.CAMPOS, W. F.VIANA, A. P.PEREIRA, M. G.2017-12-14T23:23:44Z2017-12-14T23:23:44Z2017-12-1420172017-12-14T23:23:44Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, Ribeirão Preto, v. 16, n. 2, p. 1-9, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/108261410.4238/gmr16029586enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-12-14T23:23:51Zoai:www.alice.cnptia.embrapa.br:doc/1082614Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-12-14T23:23:51falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-12-14T23:23:51Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Molecular analysis of genetic diversity among vine accessions using DNA markers.
title Molecular analysis of genetic diversity among vine accessions using DNA markers.
spellingShingle Molecular analysis of genetic diversity among vine accessions using DNA markers.
COSTA, A. F. da
Análise molecular
Videira
Marcador molecular
Variação genética
title_short Molecular analysis of genetic diversity among vine accessions using DNA markers.
title_full Molecular analysis of genetic diversity among vine accessions using DNA markers.
title_fullStr Molecular analysis of genetic diversity among vine accessions using DNA markers.
title_full_unstemmed Molecular analysis of genetic diversity among vine accessions using DNA markers.
title_sort Molecular analysis of genetic diversity among vine accessions using DNA markers.
author COSTA, A. F. da
author_facet COSTA, A. F. da
TEODORO, P. E.
BHERING, L. L.
TARDIN, F. D.
DAHER, R. F.
CAMPOS, W. F.
VIANA, A. P.
PEREIRA, M. G.
author_role author
author2 TEODORO, P. E.
BHERING, L. L.
TARDIN, F. D.
DAHER, R. F.
CAMPOS, W. F.
VIANA, A. P.
PEREIRA, M. G.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural; Universidade Federal de Viçosa; Universidade Federal de Viçosa; FLAVIO DESSAUNE TARDIN, CNPMS; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Universidade Estadual do Norte Fluminense Darcy Ribeiro.
dc.contributor.author.fl_str_mv COSTA, A. F. da
TEODORO, P. E.
BHERING, L. L.
TARDIN, F. D.
DAHER, R. F.
CAMPOS, W. F.
VIANA, A. P.
PEREIRA, M. G.
dc.subject.por.fl_str_mv Análise molecular
Videira
Marcador molecular
Variação genética
topic Análise molecular
Videira
Marcador molecular
Variação genética
description Viticulture presents a number of economic and social advantages, such as increasing employment levels and fixing the labor force in rural areas. With the aim of initiating a program of genetic improvement in grapevine from the State University of the state of Rio de Janeiro North Darcy Ribeiro, genetic diversity between 40 genotypes (varieties, rootstock, and species of different subgenera) was evaluated using Random amplified polymorphic DNA (RAPD) molecular markers. We built a matrix of binary data, whereby the presence of a band was assigned as "1" and the absence of a band was assigned as "0". The genetic distance was calculated between pairs of genotypes based on the arithmetic complement from the Jaccard Index. The results revealed the presence of considerable variability in the collection. Analysis of the genetic dissimilarity matrix revealed that the most dissimilar genotypes were Rupestris du Lot and Vitis rotundifolia because they were the most genetically distant (0.5972). The most similar were genotypes 31 (unidentified) and Rupestris du lot, which showed zero distance, confirming the results of field observations. A duplicate was confirmed, consistent with field observations, and a short distance was found between the variety "Italy" and its mutation, "Ruby". The grouping methods used were somewhat concordant.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-14T23:23:44Z
2017-12-14T23:23:44Z
2017-12-14
2017
2017-12-14T23:23:44Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 2, p. 1-9, 2017.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082614
10.4238/gmr16029586
identifier_str_mv Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 2, p. 1-9, 2017.
10.4238/gmr16029586
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082614
dc.language.iso.fl_str_mv eng
language eng
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instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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