Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1139140 https://doi.org/10.25186/.v15i.1704 |
Resumo: | The endosperm of coffee beans is an important structure which is composed of different reserve compounds. In the present work, we followed anatomical features during the reserve mobilization of Coffea Arabica L. 'Catuaí' seed samples, harvested at 20 different sites, after 0, 3, 6, 12, 24, 33 and 45 days of imbibition. Seed samples were properly fixed and stored, respectively, for histochemical and enzyme activity approach. Fixed samples were cross-sectioned for detection of starch, lipids, essential oils, oleoresins, proteins, phenolic compounds, neutral polysaccharides, alkaloids, B-1,3 and B-1,4 glucans, and lignin. Overall, based on histochemical tests and enzyme activity, seed reserve mobilization was similar among the evaluated altitudes and mountainsides. During soaking, there is an intense consumption of reserve compounds, starting at the region close to the embryo. Reserve mobilization intensifies after root protrusion, from 12 days of soaking. The intensification of the reserve consumption is concomitant with an increased activity of MDH is observed at the first 12 days, whereas SOD showed higher expression after 33 days of soaking. At the 33 rd day, little reserve is observed in the endosperm. At 45th day, cotyledon leaves are expanded, and the root system, constituted by the taproot and lateral roots, is well established. |
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Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds.Coffea ArábicaGerminaçãoCoffea arabica var. arabicaGerminationHistochemistryThe endosperm of coffee beans is an important structure which is composed of different reserve compounds. In the present work, we followed anatomical features during the reserve mobilization of Coffea Arabica L. 'Catuaí' seed samples, harvested at 20 different sites, after 0, 3, 6, 12, 24, 33 and 45 days of imbibition. Seed samples were properly fixed and stored, respectively, for histochemical and enzyme activity approach. Fixed samples were cross-sectioned for detection of starch, lipids, essential oils, oleoresins, proteins, phenolic compounds, neutral polysaccharides, alkaloids, B-1,3 and B-1,4 glucans, and lignin. Overall, based on histochemical tests and enzyme activity, seed reserve mobilization was similar among the evaluated altitudes and mountainsides. During soaking, there is an intense consumption of reserve compounds, starting at the region close to the embryo. Reserve mobilization intensifies after root protrusion, from 12 days of soaking. The intensification of the reserve consumption is concomitant with an increased activity of MDH is observed at the first 12 days, whereas SOD showed higher expression after 33 days of soaking. At the 33 rd day, little reserve is observed in the endosperm. At 45th day, cotyledon leaves are expanded, and the root system, constituted by the taproot and lateral roots, is well established.LEONARDO ARAUJO OLIVEIRA, UFV; GENAINA APARECIDA DE SOUZA, UFV; BRUNO TAVARES SILVA, UFV; ANTÔNIO AUGUSTO GOMES ROCHA, UFV; EDGARD AUGUSTO DE TOLEDO PICOLI, UFV; DIEGO DE SOUZA PEREIRA, UFLA; SÉRGIO MAURÍCIO LOPES DONZELES, EPAMIG; MARCELO DE FREITAS RIBEIRO, EPAMIG; WILLIAMS PINTO MARQUES FERREIRA, CNPCa.OLIVEIRA, L. A.SOUZA, G. A. deSILVA, B. T.ROCHA, A. A. G.PICOLI, E. A. de T.PEREIRA, D. de S.DONZELES, S. M. L.RIBEIRO, M. de F.FERREIRA, W. P. M.2022-01-18T15:00:26Z2022-01-18T15:00:26Z2022-01-182020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCoffee Science, v. 15, e151704, p. 77 -100, 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1139140https://doi.org/10.25186/.v15i.1704enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-01-18T15:00:36Zoai:www.alice.cnptia.embrapa.br:doc/1139140Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-01-18T15:00:36falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-01-18T15:00:36Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds. |
title |
Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds. |
spellingShingle |
Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds. OLIVEIRA, L. A. Coffea Arábica Germinação Coffea arabica var. arabica Germination Histochemistry |
title_short |
Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds. |
title_full |
Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds. |
title_fullStr |
Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds. |
title_full_unstemmed |
Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds. |
title_sort |
Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds. |
author |
OLIVEIRA, L. A. |
author_facet |
OLIVEIRA, L. A. SOUZA, G. A. de SILVA, B. T. ROCHA, A. A. G. PICOLI, E. A. de T. PEREIRA, D. de S. DONZELES, S. M. L. RIBEIRO, M. de F. FERREIRA, W. P. M. |
author_role |
author |
author2 |
SOUZA, G. A. de SILVA, B. T. ROCHA, A. A. G. PICOLI, E. A. de T. PEREIRA, D. de S. DONZELES, S. M. L. RIBEIRO, M. de F. FERREIRA, W. P. M. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
LEONARDO ARAUJO OLIVEIRA, UFV; GENAINA APARECIDA DE SOUZA, UFV; BRUNO TAVARES SILVA, UFV; ANTÔNIO AUGUSTO GOMES ROCHA, UFV; EDGARD AUGUSTO DE TOLEDO PICOLI, UFV; DIEGO DE SOUZA PEREIRA, UFLA; SÉRGIO MAURÍCIO LOPES DONZELES, EPAMIG; MARCELO DE FREITAS RIBEIRO, EPAMIG; WILLIAMS PINTO MARQUES FERREIRA, CNPCa. |
dc.contributor.author.fl_str_mv |
OLIVEIRA, L. A. SOUZA, G. A. de SILVA, B. T. ROCHA, A. A. G. PICOLI, E. A. de T. PEREIRA, D. de S. DONZELES, S. M. L. RIBEIRO, M. de F. FERREIRA, W. P. M. |
dc.subject.por.fl_str_mv |
Coffea Arábica Germinação Coffea arabica var. arabica Germination Histochemistry |
topic |
Coffea Arábica Germinação Coffea arabica var. arabica Germination Histochemistry |
description |
The endosperm of coffee beans is an important structure which is composed of different reserve compounds. In the present work, we followed anatomical features during the reserve mobilization of Coffea Arabica L. 'Catuaí' seed samples, harvested at 20 different sites, after 0, 3, 6, 12, 24, 33 and 45 days of imbibition. Seed samples were properly fixed and stored, respectively, for histochemical and enzyme activity approach. Fixed samples were cross-sectioned for detection of starch, lipids, essential oils, oleoresins, proteins, phenolic compounds, neutral polysaccharides, alkaloids, B-1,3 and B-1,4 glucans, and lignin. Overall, based on histochemical tests and enzyme activity, seed reserve mobilization was similar among the evaluated altitudes and mountainsides. During soaking, there is an intense consumption of reserve compounds, starting at the region close to the embryo. Reserve mobilization intensifies after root protrusion, from 12 days of soaking. The intensification of the reserve consumption is concomitant with an increased activity of MDH is observed at the first 12 days, whereas SOD showed higher expression after 33 days of soaking. At the 33 rd day, little reserve is observed in the endosperm. At 45th day, cotyledon leaves are expanded, and the root system, constituted by the taproot and lateral roots, is well established. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2022-01-18T15:00:26Z 2022-01-18T15:00:26Z 2022-01-18 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Coffee Science, v. 15, e151704, p. 77 -100, 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1139140 https://doi.org/10.25186/.v15i.1704 |
identifier_str_mv |
Coffee Science, v. 15, e151704, p. 77 -100, 2020. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1139140 https://doi.org/10.25186/.v15i.1704 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503516422668288 |