Limited vegetative compatibility as a cause of somatic recombination in: Trichoderma pseudokoningii.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/916731 |
Resumo: | With the aim of a better characterization of the somatic recombination process in Trichoderma pseudokoningii, a progeny from crossings between T. pseudokoningii strains contrasting for auxotroph markers was characterized by RAPD markers and PFGE (electrophoretic karyotype). Cytological studies of the conidia, conidiogenesis and heterokaryotic colonies were also performed. The genotypes of the majority of the recombinant strains analyzed were similar to only one of the parental strains and the low frequency of polymorphic RAPD bands suggested that the nuclear fusions may not occur into the heterokaryon. In some heterokaryotic regions the existence of intensely staining hyphae might be related to cell death. We proposed that a mechanism of somatic recombination other than parasexuality might occur, being related to limited vegetative compatibility after postfusion events, as described for other Trichoderma species. |
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spelling |
Limited vegetative compatibility as a cause of somatic recombination in: Trichoderma pseudokoningii.Somatic recombinationWith the aim of a better characterization of the somatic recombination process in Trichoderma pseudokoningii, a progeny from crossings between T. pseudokoningii strains contrasting for auxotroph markers was characterized by RAPD markers and PFGE (electrophoretic karyotype). Cytological studies of the conidia, conidiogenesis and heterokaryotic colonies were also performed. The genotypes of the majority of the recombinant strains analyzed were similar to only one of the parental strains and the low frequency of polymorphic RAPD bands suggested that the nuclear fusions may not occur into the heterokaryon. In some heterokaryotic regions the existence of intensely staining hyphae might be related to cell death. We proposed that a mechanism of somatic recombination other than parasexuality might occur, being related to limited vegetative compatibility after postfusion events, as described for other Trichoderma species.FERNANDO GOMES BARCELLOS, Universidade Paranaense - Umuarama, PR.; MARIANGELA HUNGRIA DA CUNHA, CNPSO; ALINE APARECIDA PIZZIRANI-KLEINER, USP - ESALQ.BARCELLOS, F. G.HUNGRIA, M.PIZZIRANI-KLEINER, A. A.2012-02-27T11:11:11Z2012-02-27T11:11:11Z2012-02-2720112017-07-26T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Microbiology, São Paulo, v. 42, n. 4, p. 1625-1637, Oct./Dec. 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/916731enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:34:34Zoai:www.alice.cnptia.embrapa.br:doc/916731Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:34:34falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:34:34Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Limited vegetative compatibility as a cause of somatic recombination in: Trichoderma pseudokoningii. |
title |
Limited vegetative compatibility as a cause of somatic recombination in: Trichoderma pseudokoningii. |
spellingShingle |
Limited vegetative compatibility as a cause of somatic recombination in: Trichoderma pseudokoningii. BARCELLOS, F. G. Somatic recombination |
title_short |
Limited vegetative compatibility as a cause of somatic recombination in: Trichoderma pseudokoningii. |
title_full |
Limited vegetative compatibility as a cause of somatic recombination in: Trichoderma pseudokoningii. |
title_fullStr |
Limited vegetative compatibility as a cause of somatic recombination in: Trichoderma pseudokoningii. |
title_full_unstemmed |
Limited vegetative compatibility as a cause of somatic recombination in: Trichoderma pseudokoningii. |
title_sort |
Limited vegetative compatibility as a cause of somatic recombination in: Trichoderma pseudokoningii. |
author |
BARCELLOS, F. G. |
author_facet |
BARCELLOS, F. G. HUNGRIA, M. PIZZIRANI-KLEINER, A. A. |
author_role |
author |
author2 |
HUNGRIA, M. PIZZIRANI-KLEINER, A. A. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
FERNANDO GOMES BARCELLOS, Universidade Paranaense - Umuarama, PR.; MARIANGELA HUNGRIA DA CUNHA, CNPSO; ALINE APARECIDA PIZZIRANI-KLEINER, USP - ESALQ. |
dc.contributor.author.fl_str_mv |
BARCELLOS, F. G. HUNGRIA, M. PIZZIRANI-KLEINER, A. A. |
dc.subject.por.fl_str_mv |
Somatic recombination |
topic |
Somatic recombination |
description |
With the aim of a better characterization of the somatic recombination process in Trichoderma pseudokoningii, a progeny from crossings between T. pseudokoningii strains contrasting for auxotroph markers was characterized by RAPD markers and PFGE (electrophoretic karyotype). Cytological studies of the conidia, conidiogenesis and heterokaryotic colonies were also performed. The genotypes of the majority of the recombinant strains analyzed were similar to only one of the parental strains and the low frequency of polymorphic RAPD bands suggested that the nuclear fusions may not occur into the heterokaryon. In some heterokaryotic regions the existence of intensely staining hyphae might be related to cell death. We proposed that a mechanism of somatic recombination other than parasexuality might occur, being related to limited vegetative compatibility after postfusion events, as described for other Trichoderma species. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2012-02-27T11:11:11Z 2012-02-27T11:11:11Z 2012-02-27 2017-07-26T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Journal of Microbiology, São Paulo, v. 42, n. 4, p. 1625-1637, Oct./Dec. 2011. http://www.alice.cnptia.embrapa.br/alice/handle/doc/916731 |
identifier_str_mv |
Brazilian Journal of Microbiology, São Paulo, v. 42, n. 4, p. 1625-1637, Oct./Dec. 2011. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/916731 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503439326117888 |