Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit

Detalhes bibliográficos
Autor(a) principal: CARVALHO, M. A. de F.
Data de Publicação: 2014
Outros Autores: PAIVA, R., STEIN, V. C., HERRERA, R. C., PORTO, J. M. P., VARGAS, D. P., ALVES, E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160451
https://doi.org/10.1590/S1516-8913201402555
Resumo: This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.
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spelling Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruitPassifloraceaeTissue culturePlant morphologyPassion fruitsThis work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.MILENE ALVES DE FIGUEIREDO CARVALHO, CNPCa; RENATO PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS; VANESSA CRISTINA STEIN, UNIVERSIDADE FEDERAL DE GOIÁS; RAÍRYS CRAVO HERRERA, UNIVERSIDADE FEDERAL DO PARÁ; JORGE MARCELO PADOVANI PORTO, INSTITUTO FEDERAL DO ESPÍRITO SANTO; DAIANE PEIXOTO VARGAS, EMBRAPA CLIMA TEMPERADO; EDUARDO ALVES, UNIVERSIDADE FEDERAL DE LAVRAS.CARVALHO, M. A. de F.PAIVA, R.STEIN, V. C.HERRERA, R. C.PORTO, J. M. P.VARGAS, D. P.ALVES, E.2024-01-04T19:32:12Z2024-01-04T19:32:12Z2024-01-042014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Archives of Biology and Technology, v. 57, n. 6, p. 851-859, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160451https://doi.org/10.1590/S1516-8913201402555enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-01-04T19:32:12Zoai:www.alice.cnptia.embrapa.br:doc/1160451Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542024-01-04T19:32:12falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-01-04T19:32:12Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit
title Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit
spellingShingle Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit
CARVALHO, M. A. de F.
Passifloraceae
Tissue culture
Plant morphology
Passion fruits
title_short Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit
title_full Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit
title_fullStr Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit
title_full_unstemmed Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit
title_sort Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit
author CARVALHO, M. A. de F.
author_facet CARVALHO, M. A. de F.
PAIVA, R.
STEIN, V. C.
HERRERA, R. C.
PORTO, J. M. P.
VARGAS, D. P.
ALVES, E.
author_role author
author2 PAIVA, R.
STEIN, V. C.
HERRERA, R. C.
PORTO, J. M. P.
VARGAS, D. P.
ALVES, E.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv MILENE ALVES DE FIGUEIREDO CARVALHO, CNPCa; RENATO PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS; VANESSA CRISTINA STEIN, UNIVERSIDADE FEDERAL DE GOIÁS; RAÍRYS CRAVO HERRERA, UNIVERSIDADE FEDERAL DO PARÁ; JORGE MARCELO PADOVANI PORTO, INSTITUTO FEDERAL DO ESPÍRITO SANTO; DAIANE PEIXOTO VARGAS, EMBRAPA CLIMA TEMPERADO; EDUARDO ALVES, UNIVERSIDADE FEDERAL DE LAVRAS.
dc.contributor.author.fl_str_mv CARVALHO, M. A. de F.
PAIVA, R.
STEIN, V. C.
HERRERA, R. C.
PORTO, J. M. P.
VARGAS, D. P.
ALVES, E.
dc.subject.por.fl_str_mv Passifloraceae
Tissue culture
Plant morphology
Passion fruits
topic Passifloraceae
Tissue culture
Plant morphology
Passion fruits
description This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.
publishDate 2014
dc.date.none.fl_str_mv 2014
2024-01-04T19:32:12Z
2024-01-04T19:32:12Z
2024-01-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Brazilian Archives of Biology and Technology, v. 57, n. 6, p. 851-859, 2014.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160451
https://doi.org/10.1590/S1516-8913201402555
identifier_str_mv Brazilian Archives of Biology and Technology, v. 57, n. 6, p. 851-859, 2014.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160451
https://doi.org/10.1590/S1516-8913201402555
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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