Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160451 https://doi.org/10.1590/S1516-8913201402555 |
Resumo: | This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli. |
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Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruitPassifloraceaeTissue culturePlant morphologyPassion fruitsThis work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.MILENE ALVES DE FIGUEIREDO CARVALHO, CNPCa; RENATO PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS; VANESSA CRISTINA STEIN, UNIVERSIDADE FEDERAL DE GOIÁS; RAÍRYS CRAVO HERRERA, UNIVERSIDADE FEDERAL DO PARÁ; JORGE MARCELO PADOVANI PORTO, INSTITUTO FEDERAL DO ESPÍRITO SANTO; DAIANE PEIXOTO VARGAS, EMBRAPA CLIMA TEMPERADO; EDUARDO ALVES, UNIVERSIDADE FEDERAL DE LAVRAS.CARVALHO, M. A. de F.PAIVA, R.STEIN, V. C.HERRERA, R. C.PORTO, J. M. P.VARGAS, D. P.ALVES, E.2024-01-04T19:32:12Z2024-01-04T19:32:12Z2024-01-042014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Archives of Biology and Technology, v. 57, n. 6, p. 851-859, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160451https://doi.org/10.1590/S1516-8913201402555enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-01-04T19:32:12Zoai:www.alice.cnptia.embrapa.br:doc/1160451Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542024-01-04T19:32:12falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-01-04T19:32:12Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit |
title |
Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit |
spellingShingle |
Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit CARVALHO, M. A. de F. Passifloraceae Tissue culture Plant morphology Passion fruits |
title_short |
Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit |
title_full |
Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit |
title_fullStr |
Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit |
title_full_unstemmed |
Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit |
title_sort |
Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit |
author |
CARVALHO, M. A. de F. |
author_facet |
CARVALHO, M. A. de F. PAIVA, R. STEIN, V. C. HERRERA, R. C. PORTO, J. M. P. VARGAS, D. P. ALVES, E. |
author_role |
author |
author2 |
PAIVA, R. STEIN, V. C. HERRERA, R. C. PORTO, J. M. P. VARGAS, D. P. ALVES, E. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
MILENE ALVES DE FIGUEIREDO CARVALHO, CNPCa; RENATO PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS; VANESSA CRISTINA STEIN, UNIVERSIDADE FEDERAL DE GOIÁS; RAÍRYS CRAVO HERRERA, UNIVERSIDADE FEDERAL DO PARÁ; JORGE MARCELO PADOVANI PORTO, INSTITUTO FEDERAL DO ESPÍRITO SANTO; DAIANE PEIXOTO VARGAS, EMBRAPA CLIMA TEMPERADO; EDUARDO ALVES, UNIVERSIDADE FEDERAL DE LAVRAS. |
dc.contributor.author.fl_str_mv |
CARVALHO, M. A. de F. PAIVA, R. STEIN, V. C. HERRERA, R. C. PORTO, J. M. P. VARGAS, D. P. ALVES, E. |
dc.subject.por.fl_str_mv |
Passifloraceae Tissue culture Plant morphology Passion fruits |
topic |
Passifloraceae Tissue culture Plant morphology Passion fruits |
description |
This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2024-01-04T19:32:12Z 2024-01-04T19:32:12Z 2024-01-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Archives of Biology and Technology, v. 57, n. 6, p. 851-859, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160451 https://doi.org/10.1590/S1516-8913201402555 |
identifier_str_mv |
Brazilian Archives of Biology and Technology, v. 57, n. 6, p. 851-859, 2014. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160451 https://doi.org/10.1590/S1516-8913201402555 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503555327983616 |