Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001427 http://dx.doi.org/10.4238/2014.November.27.7 |
Resumo: | Drought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice. |
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Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought.ArrozOryza sativaDoença de plantaBrusoneGenética molecularSecaGene expressionRiceMagnaporthe oryzaeBlast diseaseDrought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice.ROSANGELA BEVITORI, CNPAF; MARILIA BARROS OLIVEIRA, doutoranda UFG; MARIA FATIMA GROSSI DE SA, CENARGEN; ANNA CRISTINA LANNA, CNPAF; RICARDO DIÓGENES DA SILVEIRA; SILVANA PETROFEZA, UFG.BEVITORI, R.OLIVEIRA, M. B.GROSSI-DE-SÁ, M. F.LANNA, A. C.SILVEIRA, R. D. daPETROFEZA, S.2014-12-03T11:11:11Z2014-12-03T11:11:11Z2014-12-0320142014-12-03T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 13, n. 4, p. 9795-9805, 2014.1676-5680http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001427http://dx.doi.org/10.4238/2014.November.27.7enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:45:00Zoai:www.alice.cnptia.embrapa.br:doc/1001427Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T00:45falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:45Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. |
title |
Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. |
spellingShingle |
Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. BEVITORI, R. Arroz Oryza sativa Doença de planta Brusone Genética molecular Seca Gene expression Rice Magnaporthe oryzae Blast disease |
title_short |
Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. |
title_full |
Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. |
title_fullStr |
Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. |
title_full_unstemmed |
Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. |
title_sort |
Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. |
author |
BEVITORI, R. |
author_facet |
BEVITORI, R. OLIVEIRA, M. B. GROSSI-DE-SÁ, M. F. LANNA, A. C. SILVEIRA, R. D. da PETROFEZA, S. |
author_role |
author |
author2 |
OLIVEIRA, M. B. GROSSI-DE-SÁ, M. F. LANNA, A. C. SILVEIRA, R. D. da PETROFEZA, S. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
ROSANGELA BEVITORI, CNPAF; MARILIA BARROS OLIVEIRA, doutoranda UFG; MARIA FATIMA GROSSI DE SA, CENARGEN; ANNA CRISTINA LANNA, CNPAF; RICARDO DIÓGENES DA SILVEIRA; SILVANA PETROFEZA, UFG. |
dc.contributor.author.fl_str_mv |
BEVITORI, R. OLIVEIRA, M. B. GROSSI-DE-SÁ, M. F. LANNA, A. C. SILVEIRA, R. D. da PETROFEZA, S. |
dc.subject.por.fl_str_mv |
Arroz Oryza sativa Doença de planta Brusone Genética molecular Seca Gene expression Rice Magnaporthe oryzae Blast disease |
topic |
Arroz Oryza sativa Doença de planta Brusone Genética molecular Seca Gene expression Rice Magnaporthe oryzae Blast disease |
description |
Drought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-03T11:11:11Z 2014-12-03T11:11:11Z 2014-12-03 2014 2014-12-03T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, v. 13, n. 4, p. 9795-9805, 2014. 1676-5680 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001427 http://dx.doi.org/10.4238/2014.November.27.7 |
identifier_str_mv |
Genetics and Molecular Research, v. 13, n. 4, p. 9795-9805, 2014. 1676-5680 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001427 http://dx.doi.org/10.4238/2014.November.27.7 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503397923094528 |