An appraisal of five methods for the measurement of the fungal population in soil treated with chlorothalonil.

• Autor(a) principal: VIEIRA, R. F.
• Data de Publicação: 2000
• Outros Autores: SILVA, C. M. M., MAIA, A. de H. N., FAY, E. F., COELHO, K. C.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153084
• topic: Fungicide; Living hyphal length; Chlorothalonil; Ergosterol; Microbial biomass; Fungi; Population

An experiment was carried out in a greenhouse with the aim of determining an acceptable method to quantify the fungal population in a soil treated with the fungicide chlorothalonil. Doses of the fungicide ranging from 12 to 96mg AIgÿ1 soil were applied and microbial biomass carbon (C), soil ergosterol content, living hyphal length, as well as counts of total and cellulolytic fungi colonies, were measured 1, 2, 3, 4 and 5 weeks after application. At the end of 16 weeks a new evaluation was done using the three ®rst methods. The microbial biomass C was the least sensitive parameter and, on balance, the living hyphal length was the most sensitive parameter for demonstrating effects of chlorothalonil on the fungal population. Some problems related to the ef®ciency of the ergosterol content and living hyphal length measurements in the evaluation of the effect of the fungicide on the fungal micro¯ora are discussed, as is the need to compare short-term (0±5 weeks after treatment) with longer-term (16 weeks) results.