Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction.

Detalhes bibliográficos
Autor(a) principal: MARTINS, F. A.
Data de Publicação: 2009
Outros Autores: CARNEIRO, P. C. S., GUIMARAES, C. T., MAGALHAES, J. V. D., CARNEIRO, J. E. S., CRUZ, C. D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/513749
Resumo: The lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, considering that heterozygote (Aa) has AAa endosperm, when the female genitor donates the A or a allele, respectivelly. We used semiquantitative polymerase chain reaction to determine allele dosages in DNA mixtures, by simulating the observed conditions for endospermic tissue. Semiquantitative polymerase chain reaction on agarose gels, along with regression analysis, allowed differentiation of the samples according to the amount of DNA. This type of information will help decrease the number of non-informative individuals in quantitative trait locus mapping of outbred populations, thereby increasing mapping accuracy.
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spelling Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction.MilhoZea maysGenética vegetalThe lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, considering that heterozygote (Aa) has AAa endosperm, when the female genitor donates the A or a allele, respectivelly. We used semiquantitative polymerase chain reaction to determine allele dosages in DNA mixtures, by simulating the observed conditions for endospermic tissue. Semiquantitative polymerase chain reaction on agarose gels, along with regression analysis, allowed differentiation of the samples according to the amount of DNA. This type of information will help decrease the number of non-informative individuals in quantitative trait locus mapping of outbred populations, thereby increasing mapping accuracy.Francielle Alline Martins, Universidade Federal de Viçosa; Pedro Crescêncio Souza Carneiro, Universidade Federal de Viçosa; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS; José Eustáquio de Souza Caneiro, Universidade Federal de Viçosa; Cosme Damião Cruz, Universidade Federal de Viçosa.MARTINS, F. A.CARNEIRO, P. C. S.GUIMARAES, C. T.MAGALHAES, J. V. D.CARNEIRO, J. E. S.CRUZ, C. D.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-04-10T11:11:11Z2009-10-1420092018-07-13T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, Ribeirão Preto, v. 8, n. 1, p. 319-327, 2009.http://www.alice.cnptia.embrapa.br/alice/handle/doc/513749enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:37:32Zoai:www.alice.cnptia.embrapa.br:doc/513749Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:37:32falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:37:32Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction.
title Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction.
spellingShingle Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction.
MARTINS, F. A.
Milho
Zea mays
Genética vegetal
title_short Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction.
title_full Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction.
title_fullStr Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction.
title_full_unstemmed Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction.
title_sort Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction.
author MARTINS, F. A.
author_facet MARTINS, F. A.
CARNEIRO, P. C. S.
GUIMARAES, C. T.
MAGALHAES, J. V. D.
CARNEIRO, J. E. S.
CRUZ, C. D.
author_role author
author2 CARNEIRO, P. C. S.
GUIMARAES, C. T.
MAGALHAES, J. V. D.
CARNEIRO, J. E. S.
CRUZ, C. D.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Francielle Alline Martins, Universidade Federal de Viçosa; Pedro Crescêncio Souza Carneiro, Universidade Federal de Viçosa; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS; José Eustáquio de Souza Caneiro, Universidade Federal de Viçosa; Cosme Damião Cruz, Universidade Federal de Viçosa.
dc.contributor.author.fl_str_mv MARTINS, F. A.
CARNEIRO, P. C. S.
GUIMARAES, C. T.
MAGALHAES, J. V. D.
CARNEIRO, J. E. S.
CRUZ, C. D.
dc.subject.por.fl_str_mv Milho
Zea mays
Genética vegetal
topic Milho
Zea mays
Genética vegetal
description The lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, considering that heterozygote (Aa) has AAa endosperm, when the female genitor donates the A or a allele, respectivelly. We used semiquantitative polymerase chain reaction to determine allele dosages in DNA mixtures, by simulating the observed conditions for endospermic tissue. Semiquantitative polymerase chain reaction on agarose gels, along with regression analysis, allowed differentiation of the samples according to the amount of DNA. This type of information will help decrease the number of non-informative individuals in quantitative trait locus mapping of outbred populations, thereby increasing mapping accuracy.
publishDate 2009
dc.date.none.fl_str_mv 2009-10-14
2009
2011-04-10T11:11:11Z
2011-04-10T11:11:11Z
2011-04-10T11:11:11Z
2011-04-10T11:11:11Z
2018-07-13T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Genetics and Molecular Research, Ribeirão Preto, v. 8, n. 1, p. 319-327, 2009.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/513749
identifier_str_mv Genetics and Molecular Research, Ribeirão Preto, v. 8, n. 1, p. 319-327, 2009.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/513749
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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