Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels.

• Autor(a) principal: FUGANTI, R.
• Data de Publicação: 2010
• Outros Autores: MACHADO, M. de F. P. da S., LOPES, V. S., SILVA, J. F. V., ARIAS, C. A. A., MARIN, S. R. R., BINNECK, E., ABDELNOOR, R. V., MARCELINO, F. C., NEPOMUCENO, A. L.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/660121
• topic: Marcadores moleculares; Soybean; Nematóide de galha; Meloidogyne Javanica; Nematóide; Soja

During earlier experiments, an SSR molecularmarker (176 SoyHSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number ofAT(n) insertions inside this promoter region couldmodulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions.