Effect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination.
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/484074 |
Resumo: | In this work, a woody species [A. peregrina (L.) Speg.] was studied in order to observe the effect of ABA and GA3 at the biochemical level during the process of seed germination. Embryos incubated in sucrose solution containing ABA and/or GA3 were analyzed through SDS-PAGE to observe the mobilization pattern of storage proteins during the beginning of germination. Cotyledons isolated from seeds incubated in aqueous solutions containing ABA and/or GA3, were also analyzed through SDS-PAGE and by PAGE/Activity Gels (polyacrylamide gels copolymerized with substrate for enzymes) to observe the mobilization pattern of storage proteins and protease activity after the beginning of the germination. Results of these experiments show that ABA blocks protein mobilization by inhibiting protease activity in cotyledons. This inhibition is not sufficient to prevent germination showing that the effect of ABA on germination is not dependent on protease activity. The blockage of storage protein mobilization was also observed in embryos, but no protease activity inhibition was clearly detected. ABA was able to induce the synthesis of proteins in cotyledons but not in embryos. A polypeptide with an approximate molecular weight of 17 kD, was degraded within 6 hours in control embryos, but this degradation was blocked by ABA and GA3. Using the same concentrations of ABA and GA3 on embryos and cotyledons, the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons. Although the effects of ABA and GA3 were not so different from those shown in the literature, the behavior of 17 kD-polypeptide contradicts these reports suggesting that specific studies should be performed. |
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Effect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination.ABAGA3MobilizacaoProtease activityTropical woody plantMobilizationProteinGerminaçãoProteínagerminationIn this work, a woody species [A. peregrina (L.) Speg.] was studied in order to observe the effect of ABA and GA3 at the biochemical level during the process of seed germination. Embryos incubated in sucrose solution containing ABA and/or GA3 were analyzed through SDS-PAGE to observe the mobilization pattern of storage proteins during the beginning of germination. Cotyledons isolated from seeds incubated in aqueous solutions containing ABA and/or GA3, were also analyzed through SDS-PAGE and by PAGE/Activity Gels (polyacrylamide gels copolymerized with substrate for enzymes) to observe the mobilization pattern of storage proteins and protease activity after the beginning of the germination. Results of these experiments show that ABA blocks protein mobilization by inhibiting protease activity in cotyledons. This inhibition is not sufficient to prevent germination showing that the effect of ABA on germination is not dependent on protease activity. The blockage of storage protein mobilization was also observed in embryos, but no protease activity inhibition was clearly detected. ABA was able to induce the synthesis of proteins in cotyledons but not in embryos. A polypeptide with an approximate molecular weight of 17 kD, was degraded within 6 hours in control embryos, but this degradation was blocked by ABA and GA3. Using the same concentrations of ABA and GA3 on embryos and cotyledons, the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons. Although the effects of ABA and GA3 were not so different from those shown in the literature, the behavior of 17 kD-polypeptide contradicts these reports suggesting that specific studies should be performed.EMBRAPA-CNPMS.BARDUCHE, D.PAIVA, R.LOPES, M. A.PAIVA, E.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-04-10T11:11:11Z2000-06-2619992018-06-09T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Archives of Biology and Technology, Curitiba, v. 42, n. 2, p. 135-144, 1999.http://www.alice.cnptia.embrapa.br/alice/handle/doc/484074enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:32:50Zoai:www.alice.cnptia.embrapa.br:doc/484074Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:32:50falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:32:50Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Effect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination. |
title |
Effect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination. |
spellingShingle |
Effect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination. BARDUCHE, D. ABA GA3 Mobilizacao Protease activity Tropical woody plant Mobilization Protein Germinação Proteína germination |
title_short |
Effect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination. |
title_full |
Effect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination. |
title_fullStr |
Effect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination. |
title_full_unstemmed |
Effect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination. |
title_sort |
Effect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination. |
author |
BARDUCHE, D. |
author_facet |
BARDUCHE, D. PAIVA, R. LOPES, M. A. PAIVA, E. |
author_role |
author |
author2 |
PAIVA, R. LOPES, M. A. PAIVA, E. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
EMBRAPA-CNPMS. |
dc.contributor.author.fl_str_mv |
BARDUCHE, D. PAIVA, R. LOPES, M. A. PAIVA, E. |
dc.subject.por.fl_str_mv |
ABA GA3 Mobilizacao Protease activity Tropical woody plant Mobilization Protein Germinação Proteína germination |
topic |
ABA GA3 Mobilizacao Protease activity Tropical woody plant Mobilization Protein Germinação Proteína germination |
description |
In this work, a woody species [A. peregrina (L.) Speg.] was studied in order to observe the effect of ABA and GA3 at the biochemical level during the process of seed germination. Embryos incubated in sucrose solution containing ABA and/or GA3 were analyzed through SDS-PAGE to observe the mobilization pattern of storage proteins during the beginning of germination. Cotyledons isolated from seeds incubated in aqueous solutions containing ABA and/or GA3, were also analyzed through SDS-PAGE and by PAGE/Activity Gels (polyacrylamide gels copolymerized with substrate for enzymes) to observe the mobilization pattern of storage proteins and protease activity after the beginning of the germination. Results of these experiments show that ABA blocks protein mobilization by inhibiting protease activity in cotyledons. This inhibition is not sufficient to prevent germination showing that the effect of ABA on germination is not dependent on protease activity. The blockage of storage protein mobilization was also observed in embryos, but no protease activity inhibition was clearly detected. ABA was able to induce the synthesis of proteins in cotyledons but not in embryos. A polypeptide with an approximate molecular weight of 17 kD, was degraded within 6 hours in control embryos, but this degradation was blocked by ABA and GA3. Using the same concentrations of ABA and GA3 on embryos and cotyledons, the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons. Although the effects of ABA and GA3 were not so different from those shown in the literature, the behavior of 17 kD-polypeptide contradicts these reports suggesting that specific studies should be performed. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999 2000-06-26 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2018-06-09T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Archives of Biology and Technology, Curitiba, v. 42, n. 2, p. 135-144, 1999. http://www.alice.cnptia.embrapa.br/alice/handle/doc/484074 |
identifier_str_mv |
Brazilian Archives of Biology and Technology, Curitiba, v. 42, n. 2, p. 135-144, 1999. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/484074 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503354862272512 |