Natural antisense transcripts in plants: a review and identification in soybean infected with Phakopsora pachyrhizi SuperSAGE Library.
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/962186 |
Resumo: | Natural antisense ranscripts (NAT) are RNA molecules complementary to other endogenous RNAs. They are capable of regulating the expression of target genes at different levels (transcription, mRNA stability, translation, etc.). Such a property makes them ideal for interventions in organisms? metabolism. The present study reviewed plant NAT aspects, including features, availability and genesis, conservation and distribution, coding capacity, NAT pair expression, and functions. Besides, an in silico identification of NATs pairs was presented, using deepSuperSAGE libraries of soybean infected or not with Phakopsora pachyrhizi.Resultsshowed that around 1/3 of the 77,903 predicted trans-NATs (by PlantsNATsDB database) detected had unitags mapped in both sequences ofeachpair.Thesame1/3ofthe436foreseencis-NATs showed unitags anchored in both sequences of the related pairs. For those unitags mapped in NAT pairs, a modulation expression was assigned as upregulated, downregulated, or constitutive, based on the statistical analysis (𝑃 < 0.05). As a result, the infected treatment promoted the expression of 2,313 trans-NATs pairs comprising unitags exclusively fromthat library (1,326 pairs had unitags only found in the mock library). To understand the regulation of these NAT pairs could be a key aspect in the ASR plant response. |
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Natural antisense transcripts in plants: a review and identification in soybean infected with Phakopsora pachyrhizi SuperSAGE Library.SojaNatural antisense ranscripts (NAT) are RNA molecules complementary to other endogenous RNAs. They are capable of regulating the expression of target genes at different levels (transcription, mRNA stability, translation, etc.). Such a property makes them ideal for interventions in organisms? metabolism. The present study reviewed plant NAT aspects, including features, availability and genesis, conservation and distribution, coding capacity, NAT pair expression, and functions. Besides, an in silico identification of NATs pairs was presented, using deepSuperSAGE libraries of soybean infected or not with Phakopsora pachyrhizi.Resultsshowed that around 1/3 of the 77,903 predicted trans-NATs (by PlantsNATsDB database) detected had unitags mapped in both sequences ofeachpair.Thesame1/3ofthe436foreseencis-NATs showed unitags anchored in both sequences of the related pairs. For those unitags mapped in NAT pairs, a modulation expression was assigned as upregulated, downregulated, or constitutive, based on the statistical analysis (𝑃 < 0.05). As a result, the infected treatment promoted the expression of 2,313 trans-NATs pairs comprising unitags exclusively fromthat library (1,326 pairs had unitags only found in the mock library). To understand the regulation of these NAT pairs could be a key aspect in the ASR plant response.SUZANA DE ARAGÃO BRITTO-KIDO, UFPE; JOSÉ RIBAMAR COSTA FERREIRA NETO, UFPE; VALESCA PANDOLFI, UFPE; FRANCISMAR CORRÊA MARCELINO-GUIMARÃES, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI; RICARDO VILELA ABDELNOOR, CNPSO; ANA MARIA BENKO-ISEPPON, UFPE; EDERSON AKIO KIDO, UFPE.BRITTO-KIDO, S. de A.FERREIRA NETO, J. R. C.PANDOLFI, V.MARCELINO-GUIMARÃES, F. C.NEPOMUCENO, A. L.ABDELNOOR, R. V.BENKO-ISEPPON, A. M.KIDO, E. A.2013-07-16T11:11:11Z2013-07-16T11:11:11Z2013-07-1620132013-11-28T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe Scientific World Journal, v. 2013, 14 p., 2013.http://www.alice.cnptia.embrapa.br/alice/handle/doc/96218610.1155/2013/219798enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T01:56:45Zoai:www.alice.cnptia.embrapa.br:doc/962186Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T01:56:45falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T01:56:45Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Natural antisense transcripts in plants: a review and identification in soybean infected with Phakopsora pachyrhizi SuperSAGE Library. |
title |
Natural antisense transcripts in plants: a review and identification in soybean infected with Phakopsora pachyrhizi SuperSAGE Library. |
spellingShingle |
Natural antisense transcripts in plants: a review and identification in soybean infected with Phakopsora pachyrhizi SuperSAGE Library. BRITTO-KIDO, S. de A. Soja |
title_short |
Natural antisense transcripts in plants: a review and identification in soybean infected with Phakopsora pachyrhizi SuperSAGE Library. |
title_full |
Natural antisense transcripts in plants: a review and identification in soybean infected with Phakopsora pachyrhizi SuperSAGE Library. |
title_fullStr |
Natural antisense transcripts in plants: a review and identification in soybean infected with Phakopsora pachyrhizi SuperSAGE Library. |
title_full_unstemmed |
Natural antisense transcripts in plants: a review and identification in soybean infected with Phakopsora pachyrhizi SuperSAGE Library. |
title_sort |
Natural antisense transcripts in plants: a review and identification in soybean infected with Phakopsora pachyrhizi SuperSAGE Library. |
author |
BRITTO-KIDO, S. de A. |
author_facet |
BRITTO-KIDO, S. de A. FERREIRA NETO, J. R. C. PANDOLFI, V. MARCELINO-GUIMARÃES, F. C. NEPOMUCENO, A. L. ABDELNOOR, R. V. BENKO-ISEPPON, A. M. KIDO, E. A. |
author_role |
author |
author2 |
FERREIRA NETO, J. R. C. PANDOLFI, V. MARCELINO-GUIMARÃES, F. C. NEPOMUCENO, A. L. ABDELNOOR, R. V. BENKO-ISEPPON, A. M. KIDO, E. A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
SUZANA DE ARAGÃO BRITTO-KIDO, UFPE; JOSÉ RIBAMAR COSTA FERREIRA NETO, UFPE; VALESCA PANDOLFI, UFPE; FRANCISMAR CORRÊA MARCELINO-GUIMARÃES, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI; RICARDO VILELA ABDELNOOR, CNPSO; ANA MARIA BENKO-ISEPPON, UFPE; EDERSON AKIO KIDO, UFPE. |
dc.contributor.author.fl_str_mv |
BRITTO-KIDO, S. de A. FERREIRA NETO, J. R. C. PANDOLFI, V. MARCELINO-GUIMARÃES, F. C. NEPOMUCENO, A. L. ABDELNOOR, R. V. BENKO-ISEPPON, A. M. KIDO, E. A. |
dc.subject.por.fl_str_mv |
Soja |
topic |
Soja |
description |
Natural antisense ranscripts (NAT) are RNA molecules complementary to other endogenous RNAs. They are capable of regulating the expression of target genes at different levels (transcription, mRNA stability, translation, etc.). Such a property makes them ideal for interventions in organisms? metabolism. The present study reviewed plant NAT aspects, including features, availability and genesis, conservation and distribution, coding capacity, NAT pair expression, and functions. Besides, an in silico identification of NATs pairs was presented, using deepSuperSAGE libraries of soybean infected or not with Phakopsora pachyrhizi.Resultsshowed that around 1/3 of the 77,903 predicted trans-NATs (by PlantsNATsDB database) detected had unitags mapped in both sequences ofeachpair.Thesame1/3ofthe436foreseencis-NATs showed unitags anchored in both sequences of the related pairs. For those unitags mapped in NAT pairs, a modulation expression was assigned as upregulated, downregulated, or constitutive, based on the statistical analysis (𝑃 < 0.05). As a result, the infected treatment promoted the expression of 2,313 trans-NATs pairs comprising unitags exclusively fromthat library (1,326 pairs had unitags only found in the mock library). To understand the regulation of these NAT pairs could be a key aspect in the ASR plant response. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-16T11:11:11Z 2013-07-16T11:11:11Z 2013-07-16 2013 2013-11-28T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
The Scientific World Journal, v. 2013, 14 p., 2013. http://www.alice.cnptia.embrapa.br/alice/handle/doc/962186 10.1155/2013/219798 |
identifier_str_mv |
The Scientific World Journal, v. 2013, 14 p., 2013. 10.1155/2013/219798 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/962186 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503383680286720 |