Optimization of the production process of enzymatic activity of Lentinula edodes (Berk.) Pegler in holocelulases.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/987490 |
Resumo: | Issues such as fossil fuels and oil supplies have stimulated the search for renewable alternatives such as biofuels. Agricultural crop residues represent an abundant renewable resource for the future of bioethanol. For it to be a viable alternative, the second-generation ethanol which ought to provide a net energy gain, environmental benefits, should be economically viable, and also be produced in large quantities without reducing food supplies. The current difficulty of lignocellulosic biofuel production is the hydrolysis of biomass into sugar. This is a work in which the white-rot Lentinula edodes fungus secretes substantial amounts of hydrolytic enzymes and is useful for degradation of lignocellulosic biomass which have not been described yet. The objective of this investigation was to evaluate the pH effect (5, 6 and 7), agitation (0, 100 rpm and 200 rpm) and also the cultivation time (6, 9 and 12 days). The culture medium was supplemented with agro-industrial residue and the EF 52 strain of the fungus Lentinula edodes was used as a processing agent. A factorial design 22 repeating the central point was performed. Submerged cultivation was conducted in a synthetic medium and was incubated at 25?C. The total protein content was determined as well as the activity of xylanase and cellulase (endoglucanase, exoglucanase and ?-glucosidase). By Pareto diagram, the agitation and pH variables were significant for enzymatic activities. The highest enzyme expression occurred at pH values between 5.0 and 6.0 and above 100 rpm agitation. The exoglucanase was the enzyme which showed the highest activity in terms of cellulases, despite the cultivation time. Regarding the production of other enzymes and proteins, the most significant cultivation time was 12 days. |
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Optimization of the production process of enzymatic activity of Lentinula edodes (Berk.) Pegler in holocelulases.BioetanolXilanaseHidrólise enzimáticaBasidiomicetoCellulaseXylanaseBasidiomycetesCelulaseenzymatic hydrolysisbioethanolIssues such as fossil fuels and oil supplies have stimulated the search for renewable alternatives such as biofuels. Agricultural crop residues represent an abundant renewable resource for the future of bioethanol. For it to be a viable alternative, the second-generation ethanol which ought to provide a net energy gain, environmental benefits, should be economically viable, and also be produced in large quantities without reducing food supplies. The current difficulty of lignocellulosic biofuel production is the hydrolysis of biomass into sugar. This is a work in which the white-rot Lentinula edodes fungus secretes substantial amounts of hydrolytic enzymes and is useful for degradation of lignocellulosic biomass which have not been described yet. The objective of this investigation was to evaluate the pH effect (5, 6 and 7), agitation (0, 100 rpm and 200 rpm) and also the cultivation time (6, 9 and 12 days). The culture medium was supplemented with agro-industrial residue and the EF 52 strain of the fungus Lentinula edodes was used as a processing agent. A factorial design 22 repeating the central point was performed. Submerged cultivation was conducted in a synthetic medium and was incubated at 25?C. The total protein content was determined as well as the activity of xylanase and cellulase (endoglucanase, exoglucanase and ?-glucosidase). By Pareto diagram, the agitation and pH variables were significant for enzymatic activities. The highest enzyme expression occurred at pH values between 5.0 and 6.0 and above 100 rpm agitation. The exoglucanase was the enzyme which showed the highest activity in terms of cellulases, despite the cultivation time. Regarding the production of other enzymes and proteins, the most significant cultivation time was 12 days.Juliane Andressa Chicatto, FURB; Vitória Arend Castamann, FURB; CRISTIANE VIEIRA HELM, CNPF; Lorena Benathar Ballod Tavares, FURB.CHICATTO, J. A.CASTAMANN, V. A.HELM, C. V.TAVARES, L. B. B.2014-06-02T11:11:11Z2014-06-02T11:11:11Z2014-06-0220142016-09-23T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNatural Resources, v. 5, n. 6, p. 241-255, Apr. 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/98749010.4236/nr.2014.56023enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:31:13Zoai:www.alice.cnptia.embrapa.br:doc/987490Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T00:31:13falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:31:13Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Optimization of the production process of enzymatic activity of Lentinula edodes (Berk.) Pegler in holocelulases. |
title |
Optimization of the production process of enzymatic activity of Lentinula edodes (Berk.) Pegler in holocelulases. |
spellingShingle |
Optimization of the production process of enzymatic activity of Lentinula edodes (Berk.) Pegler in holocelulases. CHICATTO, J. A. Bioetanol Xilanase Hidrólise enzimática Basidiomiceto Cellulase Xylanase Basidiomycetes Celulase enzymatic hydrolysis bioethanol |
title_short |
Optimization of the production process of enzymatic activity of Lentinula edodes (Berk.) Pegler in holocelulases. |
title_full |
Optimization of the production process of enzymatic activity of Lentinula edodes (Berk.) Pegler in holocelulases. |
title_fullStr |
Optimization of the production process of enzymatic activity of Lentinula edodes (Berk.) Pegler in holocelulases. |
title_full_unstemmed |
Optimization of the production process of enzymatic activity of Lentinula edodes (Berk.) Pegler in holocelulases. |
title_sort |
Optimization of the production process of enzymatic activity of Lentinula edodes (Berk.) Pegler in holocelulases. |
author |
CHICATTO, J. A. |
author_facet |
CHICATTO, J. A. CASTAMANN, V. A. HELM, C. V. TAVARES, L. B. B. |
author_role |
author |
author2 |
CASTAMANN, V. A. HELM, C. V. TAVARES, L. B. B. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Juliane Andressa Chicatto, FURB; Vitória Arend Castamann, FURB; CRISTIANE VIEIRA HELM, CNPF; Lorena Benathar Ballod Tavares, FURB. |
dc.contributor.author.fl_str_mv |
CHICATTO, J. A. CASTAMANN, V. A. HELM, C. V. TAVARES, L. B. B. |
dc.subject.por.fl_str_mv |
Bioetanol Xilanase Hidrólise enzimática Basidiomiceto Cellulase Xylanase Basidiomycetes Celulase enzymatic hydrolysis bioethanol |
topic |
Bioetanol Xilanase Hidrólise enzimática Basidiomiceto Cellulase Xylanase Basidiomycetes Celulase enzymatic hydrolysis bioethanol |
description |
Issues such as fossil fuels and oil supplies have stimulated the search for renewable alternatives such as biofuels. Agricultural crop residues represent an abundant renewable resource for the future of bioethanol. For it to be a viable alternative, the second-generation ethanol which ought to provide a net energy gain, environmental benefits, should be economically viable, and also be produced in large quantities without reducing food supplies. The current difficulty of lignocellulosic biofuel production is the hydrolysis of biomass into sugar. This is a work in which the white-rot Lentinula edodes fungus secretes substantial amounts of hydrolytic enzymes and is useful for degradation of lignocellulosic biomass which have not been described yet. The objective of this investigation was to evaluate the pH effect (5, 6 and 7), agitation (0, 100 rpm and 200 rpm) and also the cultivation time (6, 9 and 12 days). The culture medium was supplemented with agro-industrial residue and the EF 52 strain of the fungus Lentinula edodes was used as a processing agent. A factorial design 22 repeating the central point was performed. Submerged cultivation was conducted in a synthetic medium and was incubated at 25?C. The total protein content was determined as well as the activity of xylanase and cellulase (endoglucanase, exoglucanase and ?-glucosidase). By Pareto diagram, the agitation and pH variables were significant for enzymatic activities. The highest enzyme expression occurred at pH values between 5.0 and 6.0 and above 100 rpm agitation. The exoglucanase was the enzyme which showed the highest activity in terms of cellulases, despite the cultivation time. Regarding the production of other enzymes and proteins, the most significant cultivation time was 12 days. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-02T11:11:11Z 2014-06-02T11:11:11Z 2014-06-02 2014 2016-09-23T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Natural Resources, v. 5, n. 6, p. 241-255, Apr. 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/987490 10.4236/nr.2014.56023 |
identifier_str_mv |
Natural Resources, v. 5, n. 6, p. 241-255, Apr. 2014. 10.4236/nr.2014.56023 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/987490 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503390691065856 |