Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates rhizosphere microbiome assembly and disease suppressiveness.

Detalhes bibliográficos
Autor(a) principal: COSTA, L. S. A. S.
Data de Publicação: 2023
Outros Autores: FARIA, M. R. de, CHIARAMONTE, J. B., MENDES, L. W., SEPO, E., HOLLANDER, M. de, FERNANDES, J. M. C., CARRIÓN, V. J., BETTIOL, W., MAUCHLINE, T. H., RAAIJMAKERS, J. M., MENDES, R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160004
https://doi.org/10.1186/s40793-023-00529-2
Resumo: Abstract: Background -Disease suppressiveness of soils to fungal root pathogens is typically induced in the field by repeated infections of the host plant and concomitant changes in the taxonomic composition and functional traits of the rhizosphere microbiome. Here, we studied this remarkable phenomenon for Bipolaris sorokiniana in two wheat cultivars differing in resistance to this fungal root pathogen. Results: The results showed that repeated exposure of the susceptible wheat cultivar to the pathogen led to a significant reduction in disease severity after five successive growth cycles. Surprisingly, the resistant wheat cultivar, initially included as a control, showed the opposite pattern with an increase in disease severity after repeated pathogen exposure. Amplicon analyses revealed that the bacterial families Chitinophagaceae, Anaerolineaceae and Nitrosomonadaceae were associated with disease suppressiveness in the susceptible wheat cultivar; disease suppressiveness in the resistant wheat cultivar was also associated with Chitinophagaceae and a higher abundance of Comamonadaceae. Metagenome analysis led to the selection of 604 Biosynthetic Gene Clusters (BGCs), out of a total of 2,571 identified by AntiSMASH analysis, that were overrepresented when the soil entered the disease suppressive state. These BGCs are involved in the biosynthesis of terpenes, non-ribosomal peptides, polyketides, aryl polyenes and post-translationally modified peptides. Conclusion: Combining taxonomic and functional profiling we identified key changes in the rhizosphere microbiome during disease suppression. This illustrates how the host plant relies on the rhizosphere microbiome as the first line of defense to fight soil-borne pathogens. Microbial taxa and functions identified here can be used in novel strategies to control soil-borne fungal pathogens.
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spelling Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates rhizosphere microbiome assembly and disease suppressiveness.Soilborne pathogensPlant disease suppressionSoil microbiomeDoença FúngicaRizosferaMicrobiologia do SoloControle MicrobianoWheatFungal diseases of plantsBipolaris sorokinianaSuppressive soilsMicrobiomeBacterial communitiesFungal communitiesAbstract: Background -Disease suppressiveness of soils to fungal root pathogens is typically induced in the field by repeated infections of the host plant and concomitant changes in the taxonomic composition and functional traits of the rhizosphere microbiome. Here, we studied this remarkable phenomenon for Bipolaris sorokiniana in two wheat cultivars differing in resistance to this fungal root pathogen. Results: The results showed that repeated exposure of the susceptible wheat cultivar to the pathogen led to a significant reduction in disease severity after five successive growth cycles. Surprisingly, the resistant wheat cultivar, initially included as a control, showed the opposite pattern with an increase in disease severity after repeated pathogen exposure. Amplicon analyses revealed that the bacterial families Chitinophagaceae, Anaerolineaceae and Nitrosomonadaceae were associated with disease suppressiveness in the susceptible wheat cultivar; disease suppressiveness in the resistant wheat cultivar was also associated with Chitinophagaceae and a higher abundance of Comamonadaceae. Metagenome analysis led to the selection of 604 Biosynthetic Gene Clusters (BGCs), out of a total of 2,571 identified by AntiSMASH analysis, that were overrepresented when the soil entered the disease suppressive state. These BGCs are involved in the biosynthesis of terpenes, non-ribosomal peptides, polyketides, aryl polyenes and post-translationally modified peptides. Conclusion: Combining taxonomic and functional profiling we identified key changes in the rhizosphere microbiome during disease suppression. This illustrates how the host plant relies on the rhizosphere microbiome as the first line of defense to fight soil-borne pathogens. Microbial taxa and functions identified here can be used in novel strategies to control soil-borne fungal pathogens.LILIAN S. ABREU SOARES COSTA, NETHERLANDS INSTITUTE OF ECOLOGY; MÍRIAN RABELO DE FARIA; JOSIANE BARROS CHIARAMONTE; LUCAS W. MENDES, UNIVERSIDADE DE SÃO PAULO; EDIS SEPO, NETHERLANDS INSTITUTE OF ECOLOGY; MATTIAS DE HOLLANDER, NETHERLANDS INSTITUTE OF ECOLOGY; JOSE MAURICIO CUNHA FERNANDES, CNPT; VÍCTOR J. CARRIÓN, UNIVERSIDAD DE MÁLAGA; WAGNER BETTIOL, CNPMA; TIM H. MAUCHLINE, ROTHAMSTED RESEARCH; JOS M. RAAIJMAKERS, LEIDEN UNIVERSITY; RODRIGO MENDES, CNPMA.COSTA, L. S. A. S.FARIA, M. R. deCHIARAMONTE, J. B.MENDES, L. W.SEPO, E.HOLLANDER, M. deFERNANDES, J. M. C.CARRIÓN, V. J.BETTIOL, W.MAUCHLINE, T. H.RAAIJMAKERS, J. M.MENDES, R.2023-12-19T18:32:25Z2023-12-19T18:32:25Z2023-12-192023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEnvironmental Microbiome, v. 18, n. 1, p., 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160004https://doi.org/10.1186/s40793-023-00529-2enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-12-19T18:32:25Zoai:www.alice.cnptia.embrapa.br:doc/1160004Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-12-19T18:32:25falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-12-19T18:32:25Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates rhizosphere microbiome assembly and disease suppressiveness.
title Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates rhizosphere microbiome assembly and disease suppressiveness.
spellingShingle Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates rhizosphere microbiome assembly and disease suppressiveness.
COSTA, L. S. A. S.
Soilborne pathogens
Plant disease suppression
Soil microbiome
Doença Fúngica
Rizosfera
Microbiologia do Solo
Controle Microbiano
Wheat
Fungal diseases of plants
Bipolaris sorokiniana
Suppressive soils
Microbiome
Bacterial communities
Fungal communities
title_short Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates rhizosphere microbiome assembly and disease suppressiveness.
title_full Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates rhizosphere microbiome assembly and disease suppressiveness.
title_fullStr Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates rhizosphere microbiome assembly and disease suppressiveness.
title_full_unstemmed Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates rhizosphere microbiome assembly and disease suppressiveness.
title_sort Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates rhizosphere microbiome assembly and disease suppressiveness.
author COSTA, L. S. A. S.
author_facet COSTA, L. S. A. S.
FARIA, M. R. de
CHIARAMONTE, J. B.
MENDES, L. W.
SEPO, E.
HOLLANDER, M. de
FERNANDES, J. M. C.
CARRIÓN, V. J.
BETTIOL, W.
MAUCHLINE, T. H.
RAAIJMAKERS, J. M.
MENDES, R.
author_role author
author2 FARIA, M. R. de
CHIARAMONTE, J. B.
MENDES, L. W.
SEPO, E.
HOLLANDER, M. de
FERNANDES, J. M. C.
CARRIÓN, V. J.
BETTIOL, W.
MAUCHLINE, T. H.
RAAIJMAKERS, J. M.
MENDES, R.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv LILIAN S. ABREU SOARES COSTA, NETHERLANDS INSTITUTE OF ECOLOGY; MÍRIAN RABELO DE FARIA; JOSIANE BARROS CHIARAMONTE; LUCAS W. MENDES, UNIVERSIDADE DE SÃO PAULO; EDIS SEPO, NETHERLANDS INSTITUTE OF ECOLOGY; MATTIAS DE HOLLANDER, NETHERLANDS INSTITUTE OF ECOLOGY; JOSE MAURICIO CUNHA FERNANDES, CNPT; VÍCTOR J. CARRIÓN, UNIVERSIDAD DE MÁLAGA; WAGNER BETTIOL, CNPMA; TIM H. MAUCHLINE, ROTHAMSTED RESEARCH; JOS M. RAAIJMAKERS, LEIDEN UNIVERSITY; RODRIGO MENDES, CNPMA.
dc.contributor.author.fl_str_mv COSTA, L. S. A. S.
FARIA, M. R. de
CHIARAMONTE, J. B.
MENDES, L. W.
SEPO, E.
HOLLANDER, M. de
FERNANDES, J. M. C.
CARRIÓN, V. J.
BETTIOL, W.
MAUCHLINE, T. H.
RAAIJMAKERS, J. M.
MENDES, R.
dc.subject.por.fl_str_mv Soilborne pathogens
Plant disease suppression
Soil microbiome
Doença Fúngica
Rizosfera
Microbiologia do Solo
Controle Microbiano
Wheat
Fungal diseases of plants
Bipolaris sorokiniana
Suppressive soils
Microbiome
Bacterial communities
Fungal communities
topic Soilborne pathogens
Plant disease suppression
Soil microbiome
Doença Fúngica
Rizosfera
Microbiologia do Solo
Controle Microbiano
Wheat
Fungal diseases of plants
Bipolaris sorokiniana
Suppressive soils
Microbiome
Bacterial communities
Fungal communities
description Abstract: Background -Disease suppressiveness of soils to fungal root pathogens is typically induced in the field by repeated infections of the host plant and concomitant changes in the taxonomic composition and functional traits of the rhizosphere microbiome. Here, we studied this remarkable phenomenon for Bipolaris sorokiniana in two wheat cultivars differing in resistance to this fungal root pathogen. Results: The results showed that repeated exposure of the susceptible wheat cultivar to the pathogen led to a significant reduction in disease severity after five successive growth cycles. Surprisingly, the resistant wheat cultivar, initially included as a control, showed the opposite pattern with an increase in disease severity after repeated pathogen exposure. Amplicon analyses revealed that the bacterial families Chitinophagaceae, Anaerolineaceae and Nitrosomonadaceae were associated with disease suppressiveness in the susceptible wheat cultivar; disease suppressiveness in the resistant wheat cultivar was also associated with Chitinophagaceae and a higher abundance of Comamonadaceae. Metagenome analysis led to the selection of 604 Biosynthetic Gene Clusters (BGCs), out of a total of 2,571 identified by AntiSMASH analysis, that were overrepresented when the soil entered the disease suppressive state. These BGCs are involved in the biosynthesis of terpenes, non-ribosomal peptides, polyketides, aryl polyenes and post-translationally modified peptides. Conclusion: Combining taxonomic and functional profiling we identified key changes in the rhizosphere microbiome during disease suppression. This illustrates how the host plant relies on the rhizosphere microbiome as the first line of defense to fight soil-borne pathogens. Microbial taxa and functions identified here can be used in novel strategies to control soil-borne fungal pathogens.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-19T18:32:25Z
2023-12-19T18:32:25Z
2023-12-19
2023
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Environmental Microbiome, v. 18, n. 1, p., 2023.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160004
https://doi.org/10.1186/s40793-023-00529-2
identifier_str_mv Environmental Microbiome, v. 18, n. 1, p., 2023.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160004
https://doi.org/10.1186/s40793-023-00529-2
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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