Diurnal oscillations of soybean circadian clock and drought responsive genes.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/978296 |
Resumo: | Rhythms produced by the endogenous circadian clock play a critical role in allowing plants to respond and adapt to the environment. While there is a well-established regulatory link between the circadian clock and responses to abiotic stress in model plants, little is known of the circadian system in crop species like soybean. This study examines how drought impacts diurnal oscillation of both drought responsive and circadian clock genes in soybean. Drought stress induced marked changes in gene expression of several circadian clock-like components, such as LCL1-, GmELF4- and PRR-like genes, which had reduced expression in stressed plants. The same conditions produced a phase advance of expression for the GmTOC1- like, GmLUX-like and GmPRR7-like genes. Similarly, the rhythmic expression pattern of the soybean drought-responsive genes DREB-, bZIP-, GOLS-, RAB18- and Remorin-like changed significantly after plant exposure to drought. In silico analysis of promoter regions of these genes revealed the presence of cis-elements associated both with stress and circadian clock regulation. Furthermore, some soybean genes with upstream ABRE elements were responsive to abscisic acid treatment. Our results indicate that some connection between the drought response and the circadian clock may exist in soybean since (i) drought stress affects gene expression of circadian clock components and (ii) several stress responsive genes display diurnal oscillation in soybeans. |
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Diurnal oscillations of soybean circadian clock and drought responsive genes.SojaSoybeansRhythms produced by the endogenous circadian clock play a critical role in allowing plants to respond and adapt to the environment. While there is a well-established regulatory link between the circadian clock and responses to abiotic stress in model plants, little is known of the circadian system in crop species like soybean. This study examines how drought impacts diurnal oscillation of both drought responsive and circadian clock genes in soybean. Drought stress induced marked changes in gene expression of several circadian clock-like components, such as LCL1-, GmELF4- and PRR-like genes, which had reduced expression in stressed plants. The same conditions produced a phase advance of expression for the GmTOC1- like, GmLUX-like and GmPRR7-like genes. Similarly, the rhythmic expression pattern of the soybean drought-responsive genes DREB-, bZIP-, GOLS-, RAB18- and Remorin-like changed significantly after plant exposure to drought. In silico analysis of promoter regions of these genes revealed the presence of cis-elements associated both with stress and circadian clock regulation. Furthermore, some soybean genes with upstream ABRE elements were responsive to abscisic acid treatment. Our results indicate that some connection between the drought response and the circadian clock may exist in soybean since (i) drought stress affects gene expression of circadian clock components and (ii) several stress responsive genes display diurnal oscillation in soybeans.JULIANA MARCOLINO-GOMES, UEL; FABIANA APARECIDA RODRIGUES, CNPSo; RENATA FUGANTI-PAGLIARINI, CNPSO; CLAIRE BENDIX, ARS/USDA; THIAGO JONAS NAKAYAMA, UFV; BRANDON CELAYA, ARS/USDA; HUGO BRUNO CORREA MOLINARI, LABEX USA; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; FRANK G. HARMON, ARS/USDA; ALEXANDRE LIMA NEPOMUCENO, CNPSo - Labex USA.MARCOLINO-GOMES, J.RODRIGUES, F. A.FUGANTI-PAGLIARINIBENDIX, C.NAKAYAMA, T. J.CELAYA, B.MOLINARI, H. B. C.OLIVEIRA, M. C. N. deHARMON, F. G.NEPOMUCENO, A. L.2014-02-03T11:11:11Z2014-02-03T11:11:11Z2014-02-0320142015-11-11T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13 p.Plos One, v. 9, n. 1, e86402, Jan. 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/97829610.1371/journal.pone.0086402enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:59:32Zoai:www.alice.cnptia.embrapa.br:doc/978296Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T00:59:32falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:59:32Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Diurnal oscillations of soybean circadian clock and drought responsive genes. |
title |
Diurnal oscillations of soybean circadian clock and drought responsive genes. |
spellingShingle |
Diurnal oscillations of soybean circadian clock and drought responsive genes. MARCOLINO-GOMES, J. Soja Soybeans |
title_short |
Diurnal oscillations of soybean circadian clock and drought responsive genes. |
title_full |
Diurnal oscillations of soybean circadian clock and drought responsive genes. |
title_fullStr |
Diurnal oscillations of soybean circadian clock and drought responsive genes. |
title_full_unstemmed |
Diurnal oscillations of soybean circadian clock and drought responsive genes. |
title_sort |
Diurnal oscillations of soybean circadian clock and drought responsive genes. |
author |
MARCOLINO-GOMES, J. |
author_facet |
MARCOLINO-GOMES, J. RODRIGUES, F. A. FUGANTI-PAGLIARINI BENDIX, C. NAKAYAMA, T. J. CELAYA, B. MOLINARI, H. B. C. OLIVEIRA, M. C. N. de HARMON, F. G. NEPOMUCENO, A. L. |
author_role |
author |
author2 |
RODRIGUES, F. A. FUGANTI-PAGLIARINI BENDIX, C. NAKAYAMA, T. J. CELAYA, B. MOLINARI, H. B. C. OLIVEIRA, M. C. N. de HARMON, F. G. NEPOMUCENO, A. L. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
JULIANA MARCOLINO-GOMES, UEL; FABIANA APARECIDA RODRIGUES, CNPSo; RENATA FUGANTI-PAGLIARINI, CNPSO; CLAIRE BENDIX, ARS/USDA; THIAGO JONAS NAKAYAMA, UFV; BRANDON CELAYA, ARS/USDA; HUGO BRUNO CORREA MOLINARI, LABEX USA; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; FRANK G. HARMON, ARS/USDA; ALEXANDRE LIMA NEPOMUCENO, CNPSo - Labex USA. |
dc.contributor.author.fl_str_mv |
MARCOLINO-GOMES, J. RODRIGUES, F. A. FUGANTI-PAGLIARINI BENDIX, C. NAKAYAMA, T. J. CELAYA, B. MOLINARI, H. B. C. OLIVEIRA, M. C. N. de HARMON, F. G. NEPOMUCENO, A. L. |
dc.subject.por.fl_str_mv |
Soja Soybeans |
topic |
Soja Soybeans |
description |
Rhythms produced by the endogenous circadian clock play a critical role in allowing plants to respond and adapt to the environment. While there is a well-established regulatory link between the circadian clock and responses to abiotic stress in model plants, little is known of the circadian system in crop species like soybean. This study examines how drought impacts diurnal oscillation of both drought responsive and circadian clock genes in soybean. Drought stress induced marked changes in gene expression of several circadian clock-like components, such as LCL1-, GmELF4- and PRR-like genes, which had reduced expression in stressed plants. The same conditions produced a phase advance of expression for the GmTOC1- like, GmLUX-like and GmPRR7-like genes. Similarly, the rhythmic expression pattern of the soybean drought-responsive genes DREB-, bZIP-, GOLS-, RAB18- and Remorin-like changed significantly after plant exposure to drought. In silico analysis of promoter regions of these genes revealed the presence of cis-elements associated both with stress and circadian clock regulation. Furthermore, some soybean genes with upstream ABRE elements were responsive to abscisic acid treatment. Our results indicate that some connection between the drought response and the circadian clock may exist in soybean since (i) drought stress affects gene expression of circadian clock components and (ii) several stress responsive genes display diurnal oscillation in soybeans. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-03T11:11:11Z 2014-02-03T11:11:11Z 2014-02-03 2014 2015-11-11T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plos One, v. 9, n. 1, e86402, Jan. 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/978296 10.1371/journal.pone.0086402 |
identifier_str_mv |
Plos One, v. 9, n. 1, e86402, Jan. 2014. 10.1371/journal.pone.0086402 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/978296 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
13 p. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503386058457088 |