Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae).
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Publication Date: | 2023 |
Other Authors: | , , , , , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Download full: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1158428 https://doi.org/10.1590/1519-6984.236649 |
Summary: | Croton argyrophylloides Muell. Arg., from the Euphorbiaceae family, popularly known as marmeleiro prateado or sacatinga, is a plant from the Caatinga biome commonly found in Brazil?s northeastern region. The present study aimed to evaluate the antioxidant activity of the species. The phytochemical study was performed through qualitative analysis of chemical constituents and quantitative determination of the total phenol content through the Folin-Ciocalteu test. The qualitative and quantitative antioxidant tests were performed using the DPPH method (2.2 diphenyl-1-picryl hydrazil) and ferric reducing antioxidant power (FRAP). The minimum inhibitory concentration (MIC) was determined by microdilution in 96-well plates. The ethanolic extract of the leaves of C. argyrophylloides manifested antioxidant action in the quantitative DPPH test with a significant bioactivity of 84.70 AAO% in 500 µg/mL, with an EC50 of 236.79. The content of total phenolic compounds was 946.06 mg of gallic acid equivalents/g of sample, and total flavonoids was 58.11 mg of quercetin equivalents/g of sample, the result obtained for FRAP was 15294.44 µM Trolox/g of sample and ABTS was 718 μM Trolox of sample. The prospecting of the chemical constituents of the leaves of C. argyrophylloides revealed the presence of the main compounds that manifests the antioxidant activity and it was proven by the DPPH method that there is antioxidant activity in the analyzed sample, in addition to demonstrating a significant content of phenolic compounds and total flavonoid content in the species, which corroborates the antioxidant activity of the plant sample. The leaf extracts presented growth inhibition halos of 10 and 12 mm upon Staphylococcus aureus ATCC 25923. |
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Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae).Croton argyrophylloides Muell ArgPhytochemistryMinimal inhibitory concentrationAntioxidantsCroton argyrophylloides Muell. Arg., from the Euphorbiaceae family, popularly known as marmeleiro prateado or sacatinga, is a plant from the Caatinga biome commonly found in Brazil?s northeastern region. The present study aimed to evaluate the antioxidant activity of the species. The phytochemical study was performed through qualitative analysis of chemical constituents and quantitative determination of the total phenol content through the Folin-Ciocalteu test. The qualitative and quantitative antioxidant tests were performed using the DPPH method (2.2 diphenyl-1-picryl hydrazil) and ferric reducing antioxidant power (FRAP). The minimum inhibitory concentration (MIC) was determined by microdilution in 96-well plates. The ethanolic extract of the leaves of C. argyrophylloides manifested antioxidant action in the quantitative DPPH test with a significant bioactivity of 84.70 AAO% in 500 µg/mL, with an EC50 of 236.79. The content of total phenolic compounds was 946.06 mg of gallic acid equivalents/g of sample, and total flavonoids was 58.11 mg of quercetin equivalents/g of sample, the result obtained for FRAP was 15294.44 µM Trolox/g of sample and ABTS was 718 μM Trolox of sample. The prospecting of the chemical constituents of the leaves of C. argyrophylloides revealed the presence of the main compounds that manifests the antioxidant activity and it was proven by the DPPH method that there is antioxidant activity in the analyzed sample, in addition to demonstrating a significant content of phenolic compounds and total flavonoid content in the species, which corroborates the antioxidant activity of the plant sample. The leaf extracts presented growth inhibition halos of 10 and 12 mm upon Staphylococcus aureus ATCC 25923.T. S. NERI, Centro Universitário Cesmac; K. W. L. SILVA, Centro Universitário Cesmac; L. P. S. MAIOR, Centro Universitário Cesmac; S. K. OLIVEIRA-SILVA, Centro Universitário Cesmac; P. V. M. AZEVEDO, Centro Universitário Cesmac; D. C. S. GOMES, Centro Universitário Cesmac; M. A. SOUZA, Centro Universitário Cesmac; J. M. S. J. PAVÃO, Centro Universitário Cesmac; JOAO GOMES DA COSTA, CNAT; A. L. CUNHA, Universidade Federal de Alagoas; G. C. FERREIRA-JÚNIOR, Instituto Federal de Educação, Ciência e Tecnologia do Acre; T. J. MATOS-ROCHA, Centro Universitário Cesmac, Universidade Estadual de Ciências da Saúde de Alagoas; A. F. SANTOS, Centro Universitário Cesmac, Universidade Estadual de Alagoas; S. A. FONSECA, Centro Universitário Cesmac.NERI, T. S.SILVA, K. W. L.MAIOR, L. P. S.SILVA, S. K. O.AZEVEDO, P. V. M.GOMES, D. C. S.SOUZA, M. A.PAVÃO, J. M. S. J.COSTA, J. G. daCUNHA, A. L.FERREIRA JÚNIOR, G. C.ROCHA, T. J. M.SANTOS, A. F.FONSECA, S. A.2023-11-16T13:33:41Z2023-11-16T13:33:41Z2023-11-162023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Biology, v. 83, e236649, 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1158428https://doi.org/10.1590/1519-6984.236649enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-11-16T13:33:42Zoai:www.alice.cnptia.embrapa.br:doc/1158428Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-11-16T13:33:42falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-11-16T13:33:42Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae). |
title |
Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae). |
spellingShingle |
Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae). NERI, T. S. Croton argyrophylloides Muell Arg Phytochemistry Minimal inhibitory concentration Antioxidants |
title_short |
Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae). |
title_full |
Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae). |
title_fullStr |
Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae). |
title_full_unstemmed |
Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae). |
title_sort |
Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae). |
author |
NERI, T. S. |
author_facet |
NERI, T. S. SILVA, K. W. L. MAIOR, L. P. S. SILVA, S. K. O. AZEVEDO, P. V. M. GOMES, D. C. S. SOUZA, M. A. PAVÃO, J. M. S. J. COSTA, J. G. da CUNHA, A. L. FERREIRA JÚNIOR, G. C. ROCHA, T. J. M. SANTOS, A. F. FONSECA, S. A. |
author_role |
author |
author2 |
SILVA, K. W. L. MAIOR, L. P. S. SILVA, S. K. O. AZEVEDO, P. V. M. GOMES, D. C. S. SOUZA, M. A. PAVÃO, J. M. S. J. COSTA, J. G. da CUNHA, A. L. FERREIRA JÚNIOR, G. C. ROCHA, T. J. M. SANTOS, A. F. FONSECA, S. A. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
T. S. NERI, Centro Universitário Cesmac; K. W. L. SILVA, Centro Universitário Cesmac; L. P. S. MAIOR, Centro Universitário Cesmac; S. K. OLIVEIRA-SILVA, Centro Universitário Cesmac; P. V. M. AZEVEDO, Centro Universitário Cesmac; D. C. S. GOMES, Centro Universitário Cesmac; M. A. SOUZA, Centro Universitário Cesmac; J. M. S. J. PAVÃO, Centro Universitário Cesmac; JOAO GOMES DA COSTA, CNAT; A. L. CUNHA, Universidade Federal de Alagoas; G. C. FERREIRA-JÚNIOR, Instituto Federal de Educação, Ciência e Tecnologia do Acre; T. J. MATOS-ROCHA, Centro Universitário Cesmac, Universidade Estadual de Ciências da Saúde de Alagoas; A. F. SANTOS, Centro Universitário Cesmac, Universidade Estadual de Alagoas; S. A. FONSECA, Centro Universitário Cesmac. |
dc.contributor.author.fl_str_mv |
NERI, T. S. SILVA, K. W. L. MAIOR, L. P. S. SILVA, S. K. O. AZEVEDO, P. V. M. GOMES, D. C. S. SOUZA, M. A. PAVÃO, J. M. S. J. COSTA, J. G. da CUNHA, A. L. FERREIRA JÚNIOR, G. C. ROCHA, T. J. M. SANTOS, A. F. FONSECA, S. A. |
dc.subject.por.fl_str_mv |
Croton argyrophylloides Muell Arg Phytochemistry Minimal inhibitory concentration Antioxidants |
topic |
Croton argyrophylloides Muell Arg Phytochemistry Minimal inhibitory concentration Antioxidants |
description |
Croton argyrophylloides Muell. Arg., from the Euphorbiaceae family, popularly known as marmeleiro prateado or sacatinga, is a plant from the Caatinga biome commonly found in Brazil?s northeastern region. The present study aimed to evaluate the antioxidant activity of the species. The phytochemical study was performed through qualitative analysis of chemical constituents and quantitative determination of the total phenol content through the Folin-Ciocalteu test. The qualitative and quantitative antioxidant tests were performed using the DPPH method (2.2 diphenyl-1-picryl hydrazil) and ferric reducing antioxidant power (FRAP). The minimum inhibitory concentration (MIC) was determined by microdilution in 96-well plates. The ethanolic extract of the leaves of C. argyrophylloides manifested antioxidant action in the quantitative DPPH test with a significant bioactivity of 84.70 AAO% in 500 µg/mL, with an EC50 of 236.79. The content of total phenolic compounds was 946.06 mg of gallic acid equivalents/g of sample, and total flavonoids was 58.11 mg of quercetin equivalents/g of sample, the result obtained for FRAP was 15294.44 µM Trolox/g of sample and ABTS was 718 μM Trolox of sample. The prospecting of the chemical constituents of the leaves of C. argyrophylloides revealed the presence of the main compounds that manifests the antioxidant activity and it was proven by the DPPH method that there is antioxidant activity in the analyzed sample, in addition to demonstrating a significant content of phenolic compounds and total flavonoid content in the species, which corroborates the antioxidant activity of the plant sample. The leaf extracts presented growth inhibition halos of 10 and 12 mm upon Staphylococcus aureus ATCC 25923. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-16T13:33:41Z 2023-11-16T13:33:41Z 2023-11-16 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Journal of Biology, v. 83, e236649, 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1158428 https://doi.org/10.1590/1519-6984.236649 |
identifier_str_mv |
Brazilian Journal of Biology, v. 83, e236649, 2023. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1158428 https://doi.org/10.1590/1519-6984.236649 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503551984074752 |