Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil.
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162096 https://doi.org/10.48130/tia-0023-0019 |
Resumo: | The hornworm (Erinnyis ello) is an important pest of cassava and rubber tree with high capacity of migration and can be controlled with biological control agents such as baculoviruses. Baculoviruses are rod-shaped enveloped viruses with circular double-stranded DNA genomes infecting insects of the order Lepidoptera. Prior to large-scale use of certain baculovirus, it is essential to assess its genetic and morphological information. This work aimed to characterize the baculovirus isolate occurring in Erinnyis ello populations at small farms in Cruzeiro do Sul, Acre State, Brazil and to develop parameters for field management using this isolate. Viral particles were purified from infected larvae through ultracentrifugation in sucrose gradient. After treatment and negative staining with uranyl acetate 2%, viral particles were visualized using transmission electron microscope. The virus with ovicylindrical occlusion body presenting only one virion per envelope and size less than 0.5 µm was identified as a member of the genus Betabaculovirus. Viral DNA was extracted through phenol/chloroform cycles, digested with different restriction enzymes and separated with agarose gel electrophoresis. The restriction enzyme patterns obtained with the enzymes Bam HI and Hind III revealed seven and three DNA fragments, respectively; cleavage with Pst I, generated eight molar fragments and three submolar fragments and Eco RI digestion resulted in 21 fragments, with some submolar bands. Comparison of the DNA restriction pattern from ErelGV isolated in Acre with that described for ErelGV isolated from SC State showed high similarity. This isolate named as ErelGV-Acre presented potential for control of E. ello populations. |
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Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil.Rubber treeCaterpillarHornwormPlagas de plantasYucaÁrbol de gomaControl biológicoComunidades ruralesCultivo a pequeña escalaCruzeiro do Sul (AC)AcreAmazônia OcidentalWestern AmazonAmazonia OccidentalPraga de PlantaMandiocaManihot EsculentaSeringueiraHevea BrasiliensisLagartaErinnyis ElloControle BiológicoBaculovirusPequeno ProdutorPropriedade RuralPlant pestsCassavaLepidopteraBiological controlBaculoviridaeDsDNA virusesRural communitiesSmall-scale farmingThe hornworm (Erinnyis ello) is an important pest of cassava and rubber tree with high capacity of migration and can be controlled with biological control agents such as baculoviruses. Baculoviruses are rod-shaped enveloped viruses with circular double-stranded DNA genomes infecting insects of the order Lepidoptera. Prior to large-scale use of certain baculovirus, it is essential to assess its genetic and morphological information. This work aimed to characterize the baculovirus isolate occurring in Erinnyis ello populations at small farms in Cruzeiro do Sul, Acre State, Brazil and to develop parameters for field management using this isolate. Viral particles were purified from infected larvae through ultracentrifugation in sucrose gradient. After treatment and negative staining with uranyl acetate 2%, viral particles were visualized using transmission electron microscope. The virus with ovicylindrical occlusion body presenting only one virion per envelope and size less than 0.5 µm was identified as a member of the genus Betabaculovirus. Viral DNA was extracted through phenol/chloroform cycles, digested with different restriction enzymes and separated with agarose gel electrophoresis. The restriction enzyme patterns obtained with the enzymes Bam HI and Hind III revealed seven and three DNA fragments, respectively; cleavage with Pst I, generated eight molar fragments and three submolar fragments and Eco RI digestion resulted in 21 fragments, with some submolar bands. Comparison of the DNA restriction pattern from ErelGV isolated in Acre with that described for ErelGV isolated from SC State showed high similarity. This isolate named as ErelGV-Acre presented potential for control of E. ello populations.WILLIAM SIHLER, Cenargen; MARCIO MARTINELLO SANCHES, CNPGC; MURILO FAZOLIN, CPAF-AC; JOELMA LIMA VIDAL ESTRELA; MARLINDA LOBO DE SOUZA, Cenargen.SIHLER, W.SANCHES, M. M.FAZOLIN, M.ESTRELA, J. L. V.SOUZA, M. L. de2024-02-19T17:32:17Z2024-02-19T17:32:17Z2024-02-192024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTechnology in Agronomy, v. 4, e001, 2024.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162096https://doi.org/10.48130/tia-0023-0019enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-02-19T17:32:17Zoai:www.alice.cnptia.embrapa.br:doc/1162096Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542024-02-19T17:32:17falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-02-19T17:32:17Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil. |
title |
Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil. |
spellingShingle |
Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil. SIHLER, W. Rubber tree Caterpillar Hornworm Plagas de plantas Yuca Árbol de goma Control biológico Comunidades rurales Cultivo a pequeña escala Cruzeiro do Sul (AC) Acre Amazônia Ocidental Western Amazon Amazonia Occidental Praga de Planta Mandioca Manihot Esculenta Seringueira Hevea Brasiliensis Lagarta Erinnyis Ello Controle Biológico Baculovirus Pequeno Produtor Propriedade Rural Plant pests Cassava Lepidoptera Biological control Baculoviridae DsDNA viruses Rural communities Small-scale farming |
title_short |
Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil. |
title_full |
Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil. |
title_fullStr |
Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil. |
title_full_unstemmed |
Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil. |
title_sort |
Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil. |
author |
SIHLER, W. |
author_facet |
SIHLER, W. SANCHES, M. M. FAZOLIN, M. ESTRELA, J. L. V. SOUZA, M. L. de |
author_role |
author |
author2 |
SANCHES, M. M. FAZOLIN, M. ESTRELA, J. L. V. SOUZA, M. L. de |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
WILLIAM SIHLER, Cenargen; MARCIO MARTINELLO SANCHES, CNPGC; MURILO FAZOLIN, CPAF-AC; JOELMA LIMA VIDAL ESTRELA; MARLINDA LOBO DE SOUZA, Cenargen. |
dc.contributor.author.fl_str_mv |
SIHLER, W. SANCHES, M. M. FAZOLIN, M. ESTRELA, J. L. V. SOUZA, M. L. de |
dc.subject.por.fl_str_mv |
Rubber tree Caterpillar Hornworm Plagas de plantas Yuca Árbol de goma Control biológico Comunidades rurales Cultivo a pequeña escala Cruzeiro do Sul (AC) Acre Amazônia Ocidental Western Amazon Amazonia Occidental Praga de Planta Mandioca Manihot Esculenta Seringueira Hevea Brasiliensis Lagarta Erinnyis Ello Controle Biológico Baculovirus Pequeno Produtor Propriedade Rural Plant pests Cassava Lepidoptera Biological control Baculoviridae DsDNA viruses Rural communities Small-scale farming |
topic |
Rubber tree Caterpillar Hornworm Plagas de plantas Yuca Árbol de goma Control biológico Comunidades rurales Cultivo a pequeña escala Cruzeiro do Sul (AC) Acre Amazônia Ocidental Western Amazon Amazonia Occidental Praga de Planta Mandioca Manihot Esculenta Seringueira Hevea Brasiliensis Lagarta Erinnyis Ello Controle Biológico Baculovirus Pequeno Produtor Propriedade Rural Plant pests Cassava Lepidoptera Biological control Baculoviridae DsDNA viruses Rural communities Small-scale farming |
description |
The hornworm (Erinnyis ello) is an important pest of cassava and rubber tree with high capacity of migration and can be controlled with biological control agents such as baculoviruses. Baculoviruses are rod-shaped enveloped viruses with circular double-stranded DNA genomes infecting insects of the order Lepidoptera. Prior to large-scale use of certain baculovirus, it is essential to assess its genetic and morphological information. This work aimed to characterize the baculovirus isolate occurring in Erinnyis ello populations at small farms in Cruzeiro do Sul, Acre State, Brazil and to develop parameters for field management using this isolate. Viral particles were purified from infected larvae through ultracentrifugation in sucrose gradient. After treatment and negative staining with uranyl acetate 2%, viral particles were visualized using transmission electron microscope. The virus with ovicylindrical occlusion body presenting only one virion per envelope and size less than 0.5 µm was identified as a member of the genus Betabaculovirus. Viral DNA was extracted through phenol/chloroform cycles, digested with different restriction enzymes and separated with agarose gel electrophoresis. The restriction enzyme patterns obtained with the enzymes Bam HI and Hind III revealed seven and three DNA fragments, respectively; cleavage with Pst I, generated eight molar fragments and three submolar fragments and Eco RI digestion resulted in 21 fragments, with some submolar bands. Comparison of the DNA restriction pattern from ErelGV isolated in Acre with that described for ErelGV isolated from SC State showed high similarity. This isolate named as ErelGV-Acre presented potential for control of E. ello populations. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-02-19T17:32:17Z 2024-02-19T17:32:17Z 2024-02-19 2024 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Technology in Agronomy, v. 4, e001, 2024. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162096 https://doi.org/10.48130/tia-0023-0019 |
identifier_str_mv |
Technology in Agronomy, v. 4, e001, 2024. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162096 https://doi.org/10.48130/tia-0023-0019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503558570180608 |