Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).

Detalhes bibliográficos
Autor(a) principal: NASCIMENTO, A. R.
Data de Publicação: 2016
Outros Autores: SILVA, G. F., GUALBERTO, G. F., ALMEIDA, F. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1058147
http://dx.doi.org/10.4238/gmr15049228
Resumo: Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change. Based on the importance of reference genes for the relative or absolute quantification of gene transcripts, we cloned and sequenced three candidate reference genes in tambaqui, and validated a set of primers for each gene for use in real-time quantitative PCR.
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spelling Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).Colossoma MacropomumTambaquiaquaculturemolecular biologyTambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change. Based on the importance of reference genes for the relative or absolute quantification of gene transcripts, we cloned and sequenced three candidate reference genes in tambaqui, and validated a set of primers for each gene for use in real-time quantitative PCR.A. R. Nascimento, Faculdade Estácio do Amazonas; GILVAN FERREIRA DA SILVA, CPAA; G. F. Gualberto; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA.NASCIMENTO, A. R.SILVA, G. F.GUALBERTO, G. F.ALMEIDA, F. L.2016-12-07T11:11:11Z2016-12-07T11:11:11Z2016-12-0720162020-03-31T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 15, n. 4, p. 15049228, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1058147http://dx.doi.org/10.4238/gmr15049228enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:03:32Zoai:www.alice.cnptia.embrapa.br:doc/1058147Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:03:32falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:03:32Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
title Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
spellingShingle Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
NASCIMENTO, A. R.
Colossoma Macropomum
Tambaqui
aquaculture
molecular biology
title_short Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
title_full Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
title_fullStr Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
title_full_unstemmed Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
title_sort Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
author NASCIMENTO, A. R.
author_facet NASCIMENTO, A. R.
SILVA, G. F.
GUALBERTO, G. F.
ALMEIDA, F. L.
author_role author
author2 SILVA, G. F.
GUALBERTO, G. F.
ALMEIDA, F. L.
author2_role author
author
author
dc.contributor.none.fl_str_mv A. R. Nascimento, Faculdade Estácio do Amazonas; GILVAN FERREIRA DA SILVA, CPAA; G. F. Gualberto; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA.
dc.contributor.author.fl_str_mv NASCIMENTO, A. R.
SILVA, G. F.
GUALBERTO, G. F.
ALMEIDA, F. L.
dc.subject.por.fl_str_mv Colossoma Macropomum
Tambaqui
aquaculture
molecular biology
topic Colossoma Macropomum
Tambaqui
aquaculture
molecular biology
description Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change. Based on the importance of reference genes for the relative or absolute quantification of gene transcripts, we cloned and sequenced three candidate reference genes in tambaqui, and validated a set of primers for each gene for use in real-time quantitative PCR.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-07T11:11:11Z
2016-12-07T11:11:11Z
2016-12-07
2016
2020-03-31T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Genetics and Molecular Research, v. 15, n. 4, p. 15049228, 2016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1058147
http://dx.doi.org/10.4238/gmr15049228
identifier_str_mv Genetics and Molecular Research, v. 15, n. 4, p. 15049228, 2016.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1058147
http://dx.doi.org/10.4238/gmr15049228
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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