Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs.

Detalhes bibliográficos
Autor(a) principal: PEREIRA, A. E.
Data de Publicação: 2016
Outros Autores: CARNEIRO, N. P., SIEGFRIED, B. D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089001
Resumo: Corn rootworms (CRW) are the most important corn pests in the U.S. Corn Belt. Damage is caused by larval feeding on the plant roots. RNAi has been studied in CRW and has proven to be effective for the management of this insect with effects on both larval and adult stages. The objective of this study was to determine comparative susceptibility of adult and larva of Southern Corn Rootworm (SCR) and Western Corn Rootworm (WCR) to two different lethal RNAi target genes (vacuolar (v)-ATPase-A and Snf7). Adults and larvae were exposed to dsRNAs overlaid on artificial diet five times every other day. WCR larval susceptibility to vATPase-A was approximately 4-fold more tolerant than SCR, but were similar for Snf7 (2.6 ng/cm2 for both species). For adults, LC50s were generally higher for vATPase-A dsRNA relative to larvae with a 20-fold higher LC50 for WCR (SCR=33.3 ng/cm2; WCR=657.3 ng/cm2) relative to Snf7 dsRNAs (SCR=13.2 ng/cm2; WCR=60.2 ng/cm2). Gene silencing was also evaluated in adults and larvae exposed to the LC50 dsRNAs, and gene knockdown ranged from 48% up to 83% in adults fed for eight days in dsRNA, and from 55% to 89% in larvae fed for 24, 48 and 72 h, compared to control treatments. This is the first study to report LC50 values for dsRNA in WCR and SCR adults. The results indicate that both larvae and adults of both species were affected by vATPase-A and Snf7 dsRNAs suggesting that RNAi for the management of CRW should be considered for both stages.
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spelling Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs.RaizLarvaPraga de plantaGeneGenéticaCorn rootworms (CRW) are the most important corn pests in the U.S. Corn Belt. Damage is caused by larval feeding on the plant roots. RNAi has been studied in CRW and has proven to be effective for the management of this insect with effects on both larval and adult stages. The objective of this study was to determine comparative susceptibility of adult and larva of Southern Corn Rootworm (SCR) and Western Corn Rootworm (WCR) to two different lethal RNAi target genes (vacuolar (v)-ATPase-A and Snf7). Adults and larvae were exposed to dsRNAs overlaid on artificial diet five times every other day. WCR larval susceptibility to vATPase-A was approximately 4-fold more tolerant than SCR, but were similar for Snf7 (2.6 ng/cm2 for both species). For adults, LC50s were generally higher for vATPase-A dsRNA relative to larvae with a 20-fold higher LC50 for WCR (SCR=33.3 ng/cm2; WCR=657.3 ng/cm2) relative to Snf7 dsRNAs (SCR=13.2 ng/cm2; WCR=60.2 ng/cm2). Gene silencing was also evaluated in adults and larvae exposed to the LC50 dsRNAs, and gene knockdown ranged from 48% up to 83% in adults fed for eight days in dsRNA, and from 55% to 89% in larvae fed for 24, 48 and 72 h, compared to control treatments. This is the first study to report LC50 values for dsRNA in WCR and SCR adults. The results indicate that both larvae and adults of both species were affected by vATPase-A and Snf7 dsRNAs suggesting that RNAi for the management of CRW should be considered for both stages.Adriano E. Pereira, University of Nebraska; NEWTON PORTILHO CARNEIRO, CNPMS; Blair D. Siegfried, University of Florida.PEREIRA, A. E.CARNEIRO, N. P.SIEGFRIED, B. D.2018-03-14T00:34:04Z2018-03-14T00:34:04Z2018-03-1320162018-05-09T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of RNAi Gene Silencing, v. 12, p. 528-535, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089001enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-03-14T00:34:11Zoai:www.alice.cnptia.embrapa.br:doc/1089001Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-03-14T00:34:11falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-03-14T00:34:11Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs.
title Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs.
spellingShingle Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs.
PEREIRA, A. E.
Raiz
Larva
Praga de planta
Gene
Genética
title_short Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs.
title_full Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs.
title_fullStr Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs.
title_full_unstemmed Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs.
title_sort Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs.
author PEREIRA, A. E.
author_facet PEREIRA, A. E.
CARNEIRO, N. P.
SIEGFRIED, B. D.
author_role author
author2 CARNEIRO, N. P.
SIEGFRIED, B. D.
author2_role author
author
dc.contributor.none.fl_str_mv Adriano E. Pereira, University of Nebraska; NEWTON PORTILHO CARNEIRO, CNPMS; Blair D. Siegfried, University of Florida.
dc.contributor.author.fl_str_mv PEREIRA, A. E.
CARNEIRO, N. P.
SIEGFRIED, B. D.
dc.subject.por.fl_str_mv Raiz
Larva
Praga de planta
Gene
Genética
topic Raiz
Larva
Praga de planta
Gene
Genética
description Corn rootworms (CRW) are the most important corn pests in the U.S. Corn Belt. Damage is caused by larval feeding on the plant roots. RNAi has been studied in CRW and has proven to be effective for the management of this insect with effects on both larval and adult stages. The objective of this study was to determine comparative susceptibility of adult and larva of Southern Corn Rootworm (SCR) and Western Corn Rootworm (WCR) to two different lethal RNAi target genes (vacuolar (v)-ATPase-A and Snf7). Adults and larvae were exposed to dsRNAs overlaid on artificial diet five times every other day. WCR larval susceptibility to vATPase-A was approximately 4-fold more tolerant than SCR, but were similar for Snf7 (2.6 ng/cm2 for both species). For adults, LC50s were generally higher for vATPase-A dsRNA relative to larvae with a 20-fold higher LC50 for WCR (SCR=33.3 ng/cm2; WCR=657.3 ng/cm2) relative to Snf7 dsRNAs (SCR=13.2 ng/cm2; WCR=60.2 ng/cm2). Gene silencing was also evaluated in adults and larvae exposed to the LC50 dsRNAs, and gene knockdown ranged from 48% up to 83% in adults fed for eight days in dsRNA, and from 55% to 89% in larvae fed for 24, 48 and 72 h, compared to control treatments. This is the first study to report LC50 values for dsRNA in WCR and SCR adults. The results indicate that both larvae and adults of both species were affected by vATPase-A and Snf7 dsRNAs suggesting that RNAi for the management of CRW should be considered for both stages.
publishDate 2016
dc.date.none.fl_str_mv 2016
2018-03-14T00:34:04Z
2018-03-14T00:34:04Z
2018-03-13
2018-05-09T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Journal of RNAi Gene Silencing, v. 12, p. 528-535, 2016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089001
identifier_str_mv Journal of RNAi Gene Silencing, v. 12, p. 528-535, 2016.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089001
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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