First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure.
Main Author: | |
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Publication Date: | 2011 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Download full: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/901961 |
Summary: | Cyanobacterial waterblooms, such as the saxitoxin (STX) producer Cylindrospermopsis raciborskii, have been a worldwide concern in environmental health. However, the bioaccumulation of this neurotoxin in the trophic chain is not completely known. The aim of the present work was to evaluate STX bioaccumulation through chemical analyses and the toxic and trophic effects using biomarkers in the tropical freshwater fish Hoplias malabaricus. They were fed once every five days with Astyanax sp. before being subjected to intraperitoneal inoculation with STX extract (0.08 mg/100 g) obtained by lysis of toxic C. raciborskii strain (T3). After 20 days the brain was collected for acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione (GSH), lipoperoxidation (LPO), protein carbonylation (PCO), and comet assay analysis. The muscle was collected for STX chemical analysis. The activities of SOD and concentrations of PCO and LPO increased. The CAT, GST, and GPx activities decreased. Genotoxicity was observed in the experimental group. STX was not detected in muscle samples. Thus, an oxidative stress was observed in the brain, leading to the damage of lipids, proteins, and DNA. The mechanism of action of the neurotoxin in this subchronic exposure suggests an apoptotic cellular process. |
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First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure.Peixe de água doceTraíraToxinaCyanobacterial waterblooms, such as the saxitoxin (STX) producer Cylindrospermopsis raciborskii, have been a worldwide concern in environmental health. However, the bioaccumulation of this neurotoxin in the trophic chain is not completely known. The aim of the present work was to evaluate STX bioaccumulation through chemical analyses and the toxic and trophic effects using biomarkers in the tropical freshwater fish Hoplias malabaricus. They were fed once every five days with Astyanax sp. before being subjected to intraperitoneal inoculation with STX extract (0.08 mg/100 g) obtained by lysis of toxic C. raciborskii strain (T3). After 20 days the brain was collected for acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione (GSH), lipoperoxidation (LPO), protein carbonylation (PCO), and comet assay analysis. The muscle was collected for STX chemical analysis. The activities of SOD and concentrations of PCO and LPO increased. The CAT, GST, and GPx activities decreased. Genotoxicity was observed in the experimental group. STX was not detected in muscle samples. Thus, an oxidative stress was observed in the brain, leading to the damage of lipids, proteins, and DNA. The mechanism of action of the neurotoxin in this subchronic exposure suggests an apoptotic cellular process.ELIANE TIE OBA YOSHIOKA, CPAF-AP.SILVA, C. A. daOBA, E. T.RAMSDORF, W. A.MAGALHÃES, V. F.CESTARI, M. M.RIBEIRO, C. A. O.ASSIS, H. C. S. de2011-09-30T11:11:11Z2011-09-30T11:11:11Z2011-09-30T11:11:11Z2011-09-30T11:11:11Z2011-09-3020112017-05-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleToxicon, Oxford, v. 57, n. 1, p. 141-147, jan. 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/901961enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:08:49Zoai:www.alice.cnptia.embrapa.br:doc/901961Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:08:49falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:08:49Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure. |
title |
First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure. |
spellingShingle |
First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure. SILVA, C. A. da Peixe de água doce Traíra Toxina |
title_short |
First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure. |
title_full |
First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure. |
title_fullStr |
First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure. |
title_full_unstemmed |
First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure. |
title_sort |
First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure. |
author |
SILVA, C. A. da |
author_facet |
SILVA, C. A. da OBA, E. T. RAMSDORF, W. A. MAGALHÃES, V. F. CESTARI, M. M. RIBEIRO, C. A. O. ASSIS, H. C. S. de |
author_role |
author |
author2 |
OBA, E. T. RAMSDORF, W. A. MAGALHÃES, V. F. CESTARI, M. M. RIBEIRO, C. A. O. ASSIS, H. C. S. de |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
ELIANE TIE OBA YOSHIOKA, CPAF-AP. |
dc.contributor.author.fl_str_mv |
SILVA, C. A. da OBA, E. T. RAMSDORF, W. A. MAGALHÃES, V. F. CESTARI, M. M. RIBEIRO, C. A. O. ASSIS, H. C. S. de |
dc.subject.por.fl_str_mv |
Peixe de água doce Traíra Toxina |
topic |
Peixe de água doce Traíra Toxina |
description |
Cyanobacterial waterblooms, such as the saxitoxin (STX) producer Cylindrospermopsis raciborskii, have been a worldwide concern in environmental health. However, the bioaccumulation of this neurotoxin in the trophic chain is not completely known. The aim of the present work was to evaluate STX bioaccumulation through chemical analyses and the toxic and trophic effects using biomarkers in the tropical freshwater fish Hoplias malabaricus. They were fed once every five days with Astyanax sp. before being subjected to intraperitoneal inoculation with STX extract (0.08 mg/100 g) obtained by lysis of toxic C. raciborskii strain (T3). After 20 days the brain was collected for acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione (GSH), lipoperoxidation (LPO), protein carbonylation (PCO), and comet assay analysis. The muscle was collected for STX chemical analysis. The activities of SOD and concentrations of PCO and LPO increased. The CAT, GST, and GPx activities decreased. Genotoxicity was observed in the experimental group. STX was not detected in muscle samples. Thus, an oxidative stress was observed in the brain, leading to the damage of lipids, proteins, and DNA. The mechanism of action of the neurotoxin in this subchronic exposure suggests an apoptotic cellular process. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-30T11:11:11Z 2011-09-30T11:11:11Z 2011-09-30T11:11:11Z 2011-09-30T11:11:11Z 2011-09-30 2011 2017-05-29T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Toxicon, Oxford, v. 57, n. 1, p. 141-147, jan. 2011. http://www.alice.cnptia.embrapa.br/alice/handle/doc/901961 |
identifier_str_mv |
Toxicon, Oxford, v. 57, n. 1, p. 141-147, jan. 2011. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/901961 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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cg-riaa@embrapa.br |
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1794503349397094400 |