Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities.

Detalhes bibliográficos
Autor(a) principal: GRUNENNVALDT, R. L.
Data de Publicação: 2018
Outros Autores: DEGENHARDT-GOLDBACH, J., TOMASI, J. de C., SANTOS, G. D. dos, VICENTE, V. A., DESCHAMPS, C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102141
Resumo: Endophytic microorganisms live inside healthy plants and their isolation and identification may favor biotechnological applications. We isolated endophytic bacteria from Ilex paraguariensis callus cultivated in vitro for five months. Sequencing of 16S rRNA region to identify two isolates as Bacillus megaterium was used. The presence of bacterial cells in the intercellular spaces of callus cultures was detected by ultra structural analyses. The isolates were also evaluated for indole acetic acid (IAA) production and their potential in plant growth promotion of Phaseolus vulgaris L. The synthesis of IAA in the presence and absence of L-tryptophan was detected by colorimetric analyze for both isolates. In the presence of extracts from strains IPC06 and IPC07 the seedlings of P. vulgaris grew faster when compared to the control plants without the extract on in vitro tests.
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spelling Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities.Erva mate16S rRNASequencingIlex ParaguariensisEspécie NativaCultura de TecidoBiotecnologiaTissue cultureEndophytic microorganisms live inside healthy plants and their isolation and identification may favor biotechnological applications. We isolated endophytic bacteria from Ilex paraguariensis callus cultivated in vitro for five months. Sequencing of 16S rRNA region to identify two isolates as Bacillus megaterium was used. The presence of bacterial cells in the intercellular spaces of callus cultures was detected by ultra structural analyses. The isolates were also evaluated for indole acetic acid (IAA) production and their potential in plant growth promotion of Phaseolus vulgaris L. The synthesis of IAA in the presence and absence of L-tryptophan was detected by colorimetric analyze for both isolates. In the presence of extracts from strains IPC06 and IPC07 the seedlings of P. vulgaris grew faster when compared to the control plants without the extract on in vitro tests.Renata Lúcia Grunennvaldt, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; Jéssica de Cássia Tomasi, UFPR; Germana Davila dos Santos, UFPR; Vânia Aparecida Vicente, UFPR; Cícero Deschamps, UFPR.GRUNENNVALDT, R. L.DEGENHARDT-GOLDBACH, J.TOMASI, J. de C.SANTOS, G. D. dosVICENTE, V. A.DESCHAMPS, C.2018-12-22T23:31:27Z2018-12-22T23:31:27Z2018-12-2020182018-12-22T23:31:27Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBiotecnología Vegetal, v. 18, n. 1, p. 3-13, ene./mar. 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102141enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-12-22T23:31:32Zoai:www.alice.cnptia.embrapa.br:doc/1102141Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-12-22T23:31:32falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-12-22T23:31:32Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities.
title Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities.
spellingShingle Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities.
GRUNENNVALDT, R. L.
Erva mate
16S rRNA
Sequencing
Ilex Paraguariensis
Espécie Nativa
Cultura de Tecido
Biotecnologia
Tissue culture
title_short Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities.
title_full Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities.
title_fullStr Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities.
title_full_unstemmed Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities.
title_sort Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities.
author GRUNENNVALDT, R. L.
author_facet GRUNENNVALDT, R. L.
DEGENHARDT-GOLDBACH, J.
TOMASI, J. de C.
SANTOS, G. D. dos
VICENTE, V. A.
DESCHAMPS, C.
author_role author
author2 DEGENHARDT-GOLDBACH, J.
TOMASI, J. de C.
SANTOS, G. D. dos
VICENTE, V. A.
DESCHAMPS, C.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Renata Lúcia Grunennvaldt, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; Jéssica de Cássia Tomasi, UFPR; Germana Davila dos Santos, UFPR; Vânia Aparecida Vicente, UFPR; Cícero Deschamps, UFPR.
dc.contributor.author.fl_str_mv GRUNENNVALDT, R. L.
DEGENHARDT-GOLDBACH, J.
TOMASI, J. de C.
SANTOS, G. D. dos
VICENTE, V. A.
DESCHAMPS, C.
dc.subject.por.fl_str_mv Erva mate
16S rRNA
Sequencing
Ilex Paraguariensis
Espécie Nativa
Cultura de Tecido
Biotecnologia
Tissue culture
topic Erva mate
16S rRNA
Sequencing
Ilex Paraguariensis
Espécie Nativa
Cultura de Tecido
Biotecnologia
Tissue culture
description Endophytic microorganisms live inside healthy plants and their isolation and identification may favor biotechnological applications. We isolated endophytic bacteria from Ilex paraguariensis callus cultivated in vitro for five months. Sequencing of 16S rRNA region to identify two isolates as Bacillus megaterium was used. The presence of bacterial cells in the intercellular spaces of callus cultures was detected by ultra structural analyses. The isolates were also evaluated for indole acetic acid (IAA) production and their potential in plant growth promotion of Phaseolus vulgaris L. The synthesis of IAA in the presence and absence of L-tryptophan was detected by colorimetric analyze for both isolates. In the presence of extracts from strains IPC06 and IPC07 the seedlings of P. vulgaris grew faster when compared to the control plants without the extract on in vitro tests.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-22T23:31:27Z
2018-12-22T23:31:27Z
2018-12-20
2018
2018-12-22T23:31:27Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Biotecnología Vegetal, v. 18, n. 1, p. 3-13, ene./mar. 2018.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102141
identifier_str_mv Biotecnología Vegetal, v. 18, n. 1, p. 3-13, ene./mar. 2018.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102141
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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