Drought tolerance induced by the overexpression of the nuclear rbcL gene in rice.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156807 https://doi.org/10.1590/S1678-3921.pab2023.v58.03181 |
Resumo: | The objective of this work was to determine whether the overexpression of the nuclear Rubisco large subunit (rbcL) improves the drought tolerance of the genetically modified (GM) BRSMG Curinga upland rice (Oryza sativa) cultivar. GM and non-genetically modified (NGM) plants of the same cultivar were compared under the two following water treatments: well watered (WW) and water deficit (WD). The performance of the agronomic traits of GM plants, including grain yield, was superior to that of NGM plants in both treatments. By quantitative polymerase chain reaction, GM plants show a significantly higher expression of the rbcL gene in both WW and WD, as well as a larger amount of abscisic acid. With the RNAseq analysis, almost three times more upregulated genes are identified in GM plants in stage 2 after water restriction, indicating a greater protection against water deficit. The higher expression of genes related to the protection of the cellular metabolism and a series of physiological alterations may be involved in the increase in the drought tolerance of GM rice plants overexpressing the rbcL gene. |
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Drought tolerance induced by the overexpression of the nuclear rbcL gene in rice.Déficit hídricoCultivar BRSMG CuringaOryza SativaÁcido AbscisicoEngenharia GenéticaMelhoramento Genético VegetalIrrigaçãoResistência a SecaAbscisic acidGenetic engineeringDrought toleranceRiceThe objective of this work was to determine whether the overexpression of the nuclear Rubisco large subunit (rbcL) improves the drought tolerance of the genetically modified (GM) BRSMG Curinga upland rice (Oryza sativa) cultivar. GM and non-genetically modified (NGM) plants of the same cultivar were compared under the two following water treatments: well watered (WW) and water deficit (WD). The performance of the agronomic traits of GM plants, including grain yield, was superior to that of NGM plants in both treatments. By quantitative polymerase chain reaction, GM plants show a significantly higher expression of the rbcL gene in both WW and WD, as well as a larger amount of abscisic acid. With the RNAseq analysis, almost three times more upregulated genes are identified in GM plants in stage 2 after water restriction, indicating a greater protection against water deficit. The higher expression of genes related to the protection of the cellular metabolism and a series of physiological alterations may be involved in the increase in the drought tolerance of GM rice plants overexpressing the rbcL gene.JOÃO AUGUSTO VIEIRA DE OLIVEIRA, ADVANCED BACTERIAL SCIENCES Ltda.; ROSANA PEREIRA VIANELLO, CNPAF; ANNA CRISTINA LANNA, CNPAF; BEATA DEDICOVA, SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES; DHIÔVANNA CORRÊIA ROCHA, INSTITUTO AGRONÔMICO DE CAMPINAS; CLAUDIO BRONDANI, CNPAF.OLIVEIRA, J. A. V. deVIANELLO, R. P.LANNA, A. C.DEDICOVA, B.ROCHA, D. C.BRONDANI, C.2023-09-27T14:24:08Z2023-09-27T14:24:08Z2023-09-202023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePesquisa Agropecuária Brasileira, v. 58, e03181, 2023.1678-3921http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156807https://doi.org/10.1590/S1678-3921.pab2023.v58.03181enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-09-27T14:24:08Zoai:www.alice.cnptia.embrapa.br:doc/1156807Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-09-27T14:24:08falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-09-27T14:24:08Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Drought tolerance induced by the overexpression of the nuclear rbcL gene in rice. |
title |
Drought tolerance induced by the overexpression of the nuclear rbcL gene in rice. |
spellingShingle |
Drought tolerance induced by the overexpression of the nuclear rbcL gene in rice. OLIVEIRA, J. A. V. de Déficit hídrico Cultivar BRSMG Curinga Oryza Sativa Ácido Abscisico Engenharia Genética Melhoramento Genético Vegetal Irrigação Resistência a Seca Abscisic acid Genetic engineering Drought tolerance Rice |
title_short |
Drought tolerance induced by the overexpression of the nuclear rbcL gene in rice. |
title_full |
Drought tolerance induced by the overexpression of the nuclear rbcL gene in rice. |
title_fullStr |
Drought tolerance induced by the overexpression of the nuclear rbcL gene in rice. |
title_full_unstemmed |
Drought tolerance induced by the overexpression of the nuclear rbcL gene in rice. |
title_sort |
Drought tolerance induced by the overexpression of the nuclear rbcL gene in rice. |
author |
OLIVEIRA, J. A. V. de |
author_facet |
OLIVEIRA, J. A. V. de VIANELLO, R. P. LANNA, A. C. DEDICOVA, B. ROCHA, D. C. BRONDANI, C. |
author_role |
author |
author2 |
VIANELLO, R. P. LANNA, A. C. DEDICOVA, B. ROCHA, D. C. BRONDANI, C. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
JOÃO AUGUSTO VIEIRA DE OLIVEIRA, ADVANCED BACTERIAL SCIENCES Ltda.; ROSANA PEREIRA VIANELLO, CNPAF; ANNA CRISTINA LANNA, CNPAF; BEATA DEDICOVA, SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES; DHIÔVANNA CORRÊIA ROCHA, INSTITUTO AGRONÔMICO DE CAMPINAS; CLAUDIO BRONDANI, CNPAF. |
dc.contributor.author.fl_str_mv |
OLIVEIRA, J. A. V. de VIANELLO, R. P. LANNA, A. C. DEDICOVA, B. ROCHA, D. C. BRONDANI, C. |
dc.subject.por.fl_str_mv |
Déficit hídrico Cultivar BRSMG Curinga Oryza Sativa Ácido Abscisico Engenharia Genética Melhoramento Genético Vegetal Irrigação Resistência a Seca Abscisic acid Genetic engineering Drought tolerance Rice |
topic |
Déficit hídrico Cultivar BRSMG Curinga Oryza Sativa Ácido Abscisico Engenharia Genética Melhoramento Genético Vegetal Irrigação Resistência a Seca Abscisic acid Genetic engineering Drought tolerance Rice |
description |
The objective of this work was to determine whether the overexpression of the nuclear Rubisco large subunit (rbcL) improves the drought tolerance of the genetically modified (GM) BRSMG Curinga upland rice (Oryza sativa) cultivar. GM and non-genetically modified (NGM) plants of the same cultivar were compared under the two following water treatments: well watered (WW) and water deficit (WD). The performance of the agronomic traits of GM plants, including grain yield, was superior to that of NGM plants in both treatments. By quantitative polymerase chain reaction, GM plants show a significantly higher expression of the rbcL gene in both WW and WD, as well as a larger amount of abscisic acid. With the RNAseq analysis, almost three times more upregulated genes are identified in GM plants in stage 2 after water restriction, indicating a greater protection against water deficit. The higher expression of genes related to the protection of the cellular metabolism and a series of physiological alterations may be involved in the increase in the drought tolerance of GM rice plants overexpressing the rbcL gene. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-27T14:24:08Z 2023-09-27T14:24:08Z 2023-09-20 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Pesquisa Agropecuária Brasileira, v. 58, e03181, 2023. 1678-3921 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156807 https://doi.org/10.1590/S1678-3921.pab2023.v58.03181 |
identifier_str_mv |
Pesquisa Agropecuária Brasileira, v. 58, e03181, 2023. 1678-3921 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156807 https://doi.org/10.1590/S1678-3921.pab2023.v58.03181 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503549808279552 |