Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine

Detalhes bibliográficos
Autor(a) principal: Lobo, Francisco Pereira
Data de Publicação: 2016
Tipo de documento: Artigo
Título da fonte: Repositório da Produção Científica e Intelectual da Unicamp
Texto Completo: https://hdl.handle.net/20.500.12733/9415
Resumo: Agradecimentos: We thank Embrapa Beef Cattle, Post-Graduate Program in Animal Science of Federal University of Mato Grosso do Sul, National Council for Scientific and Technological Development (CNPq) and Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil, for financial support and structure. We also thank the students of the agricultural school who participated in this study
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spelling Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccineVacinas sintéticasVaccines, SyntheticRhipicephalus microplusRhipicephalus sanguineusSynthetic peptideArtigo de pesquisaAgradecimentos: We thank Embrapa Beef Cattle, Post-Graduate Program in Animal Science of Federal University of Mato Grosso do Sul, National Council for Scientific and Technological Development (CNPq) and Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil, for financial support and structure. We also thank the students of the agricultural school who participated in this studyAbstract: Tick infestation may cause several problems including affecting domestic animal health and reducing the production of meat and milk, among others. Resistance to several classes of acaricides have been reported, forcing researchers to search for alternative measures, such as vaccines against ticks, to ensure tick control while having no or at least low negative impacts on the environment and public health. However, the current commercially available vaccines in different strains of Rhipicephalus microplus are reported to be of low efficacy. Fortunately, reverse vaccinology approaches have shown positive results in the new generation of vaccines. On this basis, a synthetic peptide from the ATAQ protein, which is present in the gut and Malpighi tubes of R. microplus, was synthesized. The ATAQ proteins were isolated, characterized and sequenced from several species of the genus Rhipicephalus. The alignment showed 93.3% identity among DNA sequences of ATAQs from these species. Because of this, immunization trials with this peptide were conducted on mice, rabbits and cattle to evaluate the humoral immune response and the efficacy against Rhipicephalus sanguineus in addition to R. microplus. Based on recent results, we conclude that reverse vaccinology is a promising approach because it is more accurate and faster than conventional methods in the detection of potential antigens to use in anti-tick vaccines. It is not only applicable against R. microplus but also against tick species that play important roles in spreading other diseases. ATAQ proteins should be considered as the antigen in new trials to develop a multi-antigenic vaccine. Although these peptides behave as hapten and are not able to be recognized by the immune system on its own, using carriers and adjuvants helps its presentation and induces strong immune responses. Furthermore, an efficiency of 35% reduction in overall life cycle parameters was reported for R. microplus (98% for ELISA responder animals) and 47% for R. sanguineus. Although not yet enough to prevent the environment to infestation of ticks, this still constitutes a promising strategy that could be applied to integrated measures on tick control and in new research that develops anti-tick vaccinesCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFechadoUNIVERSIDADE ESTADUAL DE CAMPINASLobo, Francisco Pereira2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12733/9415LOBO, Francisco Pereira. Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine. Veterinary parasitology. Amsterdam : Elsevier, 2016. Vol. 221, (May., 2016), p. 30-38. Disponível em: https://hdl.handle.net/20.500.12733/9415. Acesso em: 24 mai. 2023.Inglêshttps://repositorio.unicamp.br/acervo/detalhe/1168692reponame:Repositório da Produção Científica e Intelectual da Unicampinstname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPinfo:eu-repo/semantics/openAccess2023-05-15T11:01:09Zoai:https://www.repositorio.unicamp.br/:1168692Repositório InstitucionalPUBhttp://repositorio.unicamp.br/oai/requestreposip@unicamp.bropendoar:2023-05-15T11:01:09Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)false
dc.title.none.fl_str_mv Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine
title Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine
spellingShingle Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine
Lobo, Francisco Pereira
Vacinas sintéticas
Vaccines, Synthetic
Rhipicephalus microplus
Rhipicephalus sanguineus
Synthetic peptide
Artigo de pesquisa
title_short Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine
title_full Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine
title_fullStr Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine
title_full_unstemmed Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine
title_sort Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine
author Lobo, Francisco Pereira
author_facet Lobo, Francisco Pereira
author_role author
dc.contributor.none.fl_str_mv UNIVERSIDADE ESTADUAL DE CAMPINAS
dc.contributor.author.fl_str_mv Lobo, Francisco Pereira
dc.subject.por.fl_str_mv Vacinas sintéticas
Vaccines, Synthetic
Rhipicephalus microplus
Rhipicephalus sanguineus
Synthetic peptide
Artigo de pesquisa
topic Vacinas sintéticas
Vaccines, Synthetic
Rhipicephalus microplus
Rhipicephalus sanguineus
Synthetic peptide
Artigo de pesquisa
description Agradecimentos: We thank Embrapa Beef Cattle, Post-Graduate Program in Animal Science of Federal University of Mato Grosso do Sul, National Council for Scientific and Technological Development (CNPq) and Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil, for financial support and structure. We also thank the students of the agricultural school who participated in this study
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/20.500.12733/9415
LOBO, Francisco Pereira. Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine. Veterinary parasitology. Amsterdam : Elsevier, 2016. Vol. 221, (May., 2016), p. 30-38. Disponível em: https://hdl.handle.net/20.500.12733/9415. Acesso em: 24 mai. 2023.
url https://hdl.handle.net/20.500.12733/9415
identifier_str_mv LOBO, Francisco Pereira. Design of the ATAQ peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine. Veterinary parasitology. Amsterdam : Elsevier, 2016. Vol. 221, (May., 2016), p. 30-38. Disponível em: https://hdl.handle.net/20.500.12733/9415. Acesso em: 24 mai. 2023.
dc.language.iso.fl_str_mv Inglês
language_invalid_str_mv Inglês
dc.relation.none.fl_str_mv https://repositorio.unicamp.br/acervo/detalhe/1168692
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório da Produção Científica e Intelectual da Unicamp
instname:Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
instname_str Universidade Estadual de Campinas (UNICAMP)
instacron_str UNICAMP
institution UNICAMP
reponame_str Repositório da Produção Científica e Intelectual da Unicamp
collection Repositório da Produção Científica e Intelectual da Unicamp
repository.name.fl_str_mv Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)
repository.mail.fl_str_mv reposip@unicamp.br
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